Mesenchymal stem cell conditioned media attenuates in vitro and ex vivo myocardial reperfusion injury

Angoulvant, Denis; Ivanès, Fabrice; Ferrera, René; Matthews, Phoebe G; Nataf, Serge; Ovize, Michel

doi:10.1016/j.healun.2010.08.023

Cited by 103 publications

(85 citation statements)

References 43 publications

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“…[7][8][9][10]. The beneficial effect of conditioned medium derived from mesenchymal stem cells supports this paracrine view of stem cells action [11,12]. Previous studies indicated that many of the regenerative functions of adult stem cells are mediated by the release of extracellular vesicles (EVs) [13].…”

Section: Introductionmentioning

confidence: 75%

The Angiogenic Potential of Adipose Mesenchymal Stem Cell-derived Extracellular Vesicles is modulated by Basic Fibroblast Growth Factor

Lopatina¹,

Mazzeo²,

Bruno

et al. 2014

J Stem Cell Res Ther

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Section: Introductionmentioning

confidence: 75%

The Angiogenic Potential of Adipose Mesenchymal Stem Cell-derived Extracellular Vesicles is modulated by Basic Fibroblast Growth Factor

Lopatina¹,

Mazzeo²,

Bruno

et al. 2014

J Stem Cell Res Ther

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“…11,28 It has been well known that VEGF is proangiogenic and IGF-1 stimulates mitogenesis, promotes differentiation, and inhibits apoptosis in the heart. 29,30 HGF promotes cell proliferation, motility, morphogenesis, and angiogenesis and also provides tissue protection after injury. 31 Also, previous studies have demonstrated that factors such as VEGF, IGF-1, and HGF could contribute to tissue repair through their proangiogenic and antiapoptotic roles.…”

Section: Discussionmentioning

confidence: 99%

A Regenerative Cardiac Patch Formed by Spray Painting of Biomaterials onto the Heart

Tang

Vandergriff

Wang

et al. 2017

Tissue Engineering Part C: Methods

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Layering a regenerative polymer scaffold on the surface of the heart, termed as a cardiac patch, has been proven to be effective in preserving cardiac function after myocardial infarction (MI). However, the placement of such a patch on the heart usually needs open-chest surgery, which is traumatic, therefore prevents the translation of this strategy into the clinic. We sought to device a way to apply a cardiac patch by spray painting in situ polymerizable biomaterials onto the heart with a minimally invasive procedure. To prove the concept, we used platelet fibrin gel as the ''paint'' material in a mouse model of MI. The use of the spraying system allowed for placement of a uniform cardiac patch on the heart in a mini-invasive manner without the need for sutures or glue. The spray treatment promoted cardiac repair and attenuated cardiac dysfunction after MI.

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“…These assays study the effect of secreted factors on cells, but do not specifically focus on their cardiomyogenic potential. More comprehensive in vitro approaches including all secreted factors make use of cardiomyogenic indicator cells, like cardiac progenitor cells or cardiomyocytes, to detect the stimulatory effects of conditioned media [Nakanishi et al, 2008;Angoulvant et al, 2011]. The limited availability of such stem cells and progenitor cells and their spontaneous beating activity has hampered an application for extensive screening and investigation so far.…”

Section: Discussionmentioning

confidence: 99%

“…After the cultivation of rat cardiac stem cells or cardiomyocytes with conditioned MSC culture supernatants, a positive paracrine effect on cardiomyogenesis was observed [Nakanishi et al, 2008;Angoulvant et al, 2011]. In a more general approach, Ranganath et al [2012] reviewed the MSC secretome as a potential therapeutic tool for the treatment of ischemic heart disease.…”

Section: Introductionmentioning

confidence: 99%

A P19 and P19CL6 Cell-Based Complementary Approach to Determine Paracrine Effects in Cardiac Tissue Engineering

Dehne

Adam²,

Materne³

et al. 2014

Cells Tissues Organs

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The negligible self-repair potential of the myocardium has led to cell-based tissue engineering approaches to restore heart function. There is more and more consensus that, in addition to cell development, paracrine effects in particular play a pivotal role in the repair of heart tissue. Here, we present two complementary murine P19 and P19CL6 embryonic carcinoma cell-based in vitro test approaches to study the potential of repair cells and the factors secreted by these cells to induce cardiomyogenesis. P19 cells were 3-dimensionally cultured in hanging drops and P19CL6 cells in a monolayer. Both systems, capable of inducible differentiation towards the cardiomyogenic lineage shown by the appearance of beating cells, the expression of connexin 43 and cardiac troponins T and I, were used to test the cardiomyogenesis-inducing potential of human cardiac-derived adherent proliferating (CardAP) cells, which are candidates for heart repair. CardAP cells in coculture as well as CardAP cell-conditioned medium initiated beating in P19 cells, depending on the cell composition and concentration of the medium. CardAP cell-dependent beating was not observed in P19CL6 cultures, but connexin 43 and cardiac troponin formation as well as expression of GATA-binding protein 4 indicated the dose-dependent stimulatory cardiomyogenic effect of human CardAP cells. In summary, in different ways, P19 and P19CL6 cells have shown their capability to detect paracrine effects of human CardAP cells. In a complementary approach, they could be beneficial for determining the stimulatory cardiomyogenic potential of candidate cardiac-repair cells in vitro.

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Mesenchymal stem cell conditioned media attenuates in vitro and ex vivo myocardial reperfusion injury

Cited by 103 publications

References 43 publications

The Angiogenic Potential of Adipose Mesenchymal Stem Cell-derived Extracellular Vesicles is modulated by Basic Fibroblast Growth Factor

The Angiogenic Potential of Adipose Mesenchymal Stem Cell-derived Extracellular Vesicles is modulated by Basic Fibroblast Growth Factor

A Regenerative Cardiac Patch Formed by Spray Painting of Biomaterials onto the Heart

A P19 and P19CL6 Cell-Based Complementary Approach to Determine Paracrine Effects in Cardiac Tissue Engineering

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