Mesenchymal Stem Cell Culture on Composite Hydrogels of Hydroxyapatite Nanoparticles and Photo-Crosslinking Chitosan

Abstract

Chitosan (QT) is a biopolymer that has been used in widely used in bone tissue engineering applications, demonstrating great potential for that purpose. Therefore, the present study aims to develop an in situ crosslinking hydrogel system, composed of chitosan and hydroxyapatite (HAP). Briefly, chitosan was modified, using carbodiimide chemistry, with lactobionic and azidobenzoic acid to make it soluble at physiological pH and photo-crosslinkable, respectively. The modified chitosan was mixed with HAP, in different proportions, and later exposed to UV light, yielding hydrogels. Mesenchymal stem cells, from rat bone marrow, were seeded onto the hydrogels and cultured for 4, 10 and 16 days, under osteogenic and non-osteogenic conditions. Through cell proliferation and alkaline phosphatase activity assays, and confocal microscopy, it was observed that the majority of formulations supported cell adhesion and proliferation, and a significant early osteogenic differentiation in formulations 70:30 and 50:50, QT:HAP. According to these results, the proposed photo-crosslinking system has potential for tissue engineering applications, and further specific studies are proposed for cell differentiation.

Physicochemical properties of chitosan–magnetite nanocomposites obtained with different pH

“…1 CS and derivatives exhibit biocompatibility and they are suitable for several possible biomedical applications including functionalization or coating of magnetic nanoparticles. [2][3][4][5][6][7][8][9] At present, magnetite nanoparticles (MNP) are under intense investigation for clinical applications. Magnetite nanoparticles are susceptible to pH, both in the synthesis process by co-precipitation and in post-synthesis treatments.…”

Retinal Lesion Segmentation Using Transfer Learning with an Encoder-Decoder CNN

“…Ha's biomedical applications have been of great relevance due to their high degree of biocompatibility and bioactivity. Has been employed in the manufacture of different scaffold systems, including porous structures and hydrogels, with potential applications for the regeneration of bone tissue [13] , and several methods have been developed to obtain this material, the main ones being precipitation, sol-gel, mechanochemistry, and solid-state reaction.…”

“…For this reason, it has been . Ch has been shown to be suitable for use in IT as a bone tissue graft, in the manufacture of porous scaffolds, films for superficial wounds, drug release, and in formulations for the production of hydrogels [78] .…”