2022
DOI: 10.1101/2022.08.30.505860
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Mesenchymal stem cell culture within perfusion bioreactors incorporating 3D-printed scaffolds enables improved extracellular vesicle yield with preserved bioactivity

Abstract: Extracellular vesicles (EVs) are implicated as promising therapeutics and drug delivery vehicles in various diseases. However, successful clinical translation will depend on development of scalable biomanufacturing approaches, especially due to the documented low levels of intrinsic EV-associated cargo that may necessitate repeated doses to achieve clinical benefit in certain applications. Thus, here we assessed effects of a 3D-printed scaffold-perfusion bioreactor system on the production and bioactivity of E… Show more

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Cited by 3 publications
(2 citation statements)
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“…Novel investigations have used more straightforward methods (such as flat-plate bioreactors), permitting a more accurate approach for comprehend and restraining flow-derived shear stress. However, still absence the adjustability essential for dynamic construction and experimental investigation requirements [69]. Moreover, stirred suspension bioreactors (SSBs) have been efficiently utilized to scale up MSCs manufacture.…”
Section: Msc-exos Produced By Bioreactor Production Methodsmentioning
confidence: 99%
“…Novel investigations have used more straightforward methods (such as flat-plate bioreactors), permitting a more accurate approach for comprehend and restraining flow-derived shear stress. However, still absence the adjustability essential for dynamic construction and experimental investigation requirements [69]. Moreover, stirred suspension bioreactors (SSBs) have been efficiently utilized to scale up MSCs manufacture.…”
Section: Msc-exos Produced By Bioreactor Production Methodsmentioning
confidence: 99%
“…For instance, the impact of a 3D-printed scaffold-perfusion bioreactor system on the production and bioactivity of EVs secreted by MSCs has been explored. Findings suggest that culture in a perfusion bioreactor can yield an approximate 40-80-fold increase in MSC EVs production, varying with the measurement technique, compared to traditional cell culture methods [79]. Although the application of MSC EVs produced via this system in liver disease has not yet been fully evaluated, the approach holds substantial potential for future therapeutic interventions.…”
Section: Scaling-up Isolation and Bioengineering Of Clinical-grade Ms...mentioning
confidence: 99%