Mesenchymal stem cells alleviate hydrochloric acid-induced lung injury through suppression of inflammation, oxidative stress and apoptosis in comparison to moxifloxacin and sildenafil

Abstract

IntroductionAcute lung injury (ALI) is a severe life-threatening disease causing uncontrolled pulmonary inflammation and oxidative damage. There are still no effective therapies for this disease. The aim of this study was to evaluate the protective role of mesenchymal stem cells, moxifloxacin, sildenafil or a combination of moxifloxacin and sildenafil against hydrochloric Acid (HCl) - induced ALI.MethodsHCl or saline was injected intra-tracheally and after 2 h, moxifloxacin, sildenafil, moxifloxacin + sildenafil or mesenchymal stem cells were injected. After 7 days, rats were sacrificed for evaluation of the blood chemistry and inflammation via determination of the level of oxidative stress markers, apoptosis and the histopathological alterations by H&E.ResultsIn HCl-injected rats, there were a significant increase in total white blood cells (WBCs), lymphocytes, malondialdehyde (MDA) and caspase-3 gene expression. Also, there were a significant decrease in superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and Hemeoxygenase-1 (HO-1) gene expression in lung tissue. On the other hand, treatment of lung injured rats with mesenchymal stem cell, moxifloxacin, sildenafil or a combination of moxifloxacin and sildenafil showed a significant decrease in WBCs and lymphocytes and ameliorated the histopathological changes. MDA level in lung tissue was only significantly lowered in rats treated with moxifloxacin alone or in combination with sildenafil or MSCs. GSH was just increased in rats treated with moxifloxacin, sildenafil or with MSCs. Antioxidant parameters and gene expression of HO-1 and caspase-3 were significantly modulated in rats treated with MSCs.ConclusionMSCs ameliorated the toxic effects of HCl through their ability to decrease inflammation, oxidative stress, and apoptosis in acute lung injury.

HCl-induced acute lung injury: a study of the curative role of mesenchymal stem/stromal cells and cobalt protoporphyrin

“…This gives emphasis to the relevance of restoring antioxidant levels in the effort to safeguard the most vulnerable people from severe symptoms. In a previous study, we investigated the expression of heme oxygenase-1 (HO-1) which is induced by CoPP, and it is a key player in the cellular antioxidant system in HCl-induced lung injury leading to a decrease in inflammation and in ROS production [ 6 , 11 ]. We demonstrated that the induced lung injury caused significant reduction in the expression of HO-1 at the level of mRNA, and it was upregulated by MSCs.…”

“…Aspirate content may include particles of food, gastric acid (HCl; hydrochloric acid), blood, or bacteria. Among these contents, HCl has the greatest influence on the lung injury [ 6 ].…”

Nephroprotective Effect of Mesenchymal Stem Cell-Based Therapy of Kidney Disease Induced by Toxicants

“…The MSC treatment group had a higher level of GSH, SOD, and a lower level of MDA when compared with the control group. El-Metwaly et al [ 45 ] found that MSCs increase GSH levels and reduce MDA levels in lung tissue of rats subjected to acute lung injury. Li et al [ 46 ] reported that MSCs can restore the levels of GSH and MDA in rats with chronic alcoholism, and its effects on repairing sciatic nerve were obvious.…”

Nephroprotective effect of mesenchymal stem cells in therapy of kidney disease induced by toxicant

“…MSCs treatment group got a higher level of GSH, SOD, and a lower level of MDA when compared with control group. El-Metwaly et al [40] found that MSCs can increase the GSH level and reduce the MDA level in lung tissue of acute lung injury rats. Zhang et al [41] reported that MSC effectively reduced the level of MDA, and increased SOD in the lung tissue of acute lung injury rats.…”