Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro

Oswald, Joachim; Boxberger, Sabine; Jørgensen, Bo; Feldmann, Silvia; Ehninger, Gerhard; Bornhäuser, Martin; Werner, Carsten

doi:10.1634/stemcells.22-3-377

Cited by 1,165 publications

(943 citation statements)

References 35 publications

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“…32 MSC might even contribute to tumor angiogenesis, although this latter point is critically discussed. 33,34 While the molecular processes that drive migration of MSC to tumor tissues remain unclear, the tropism of MSC to tumor tissues is suggested to be mediated, at least in part, by specific growth factors and chemokines, 30,31 such as also shown in the present study on PDGF. Consequently, to inhibit the growth of tumors in situ by MSC, genetic modification of MSC is necessary to produce high levels of anticancer agents that blunt tumor growth kinetics.…”

Section: Discussionsupporting

confidence: 55%

Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer

et al. 2008

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Genetic modification of human bone marrow mesenchymal stem cells (MSC) is highly valuable for their exploitation in basic science and therapeutic applications, for example in cancer. We present here a new, fast and easy-to-use method to enrich a functional population of lentiviral (LV)-transduced MSC expressing enhanced green fluorescent protein (eGFP). We replaced the eGFP gene by a fusion gene of puromycin acetyltransferase and eGFP. Upon LV gene transfer and puromycin selection, we quickly obtained a pure transduced MSC population, in which growth, differentiation capacity and migration preferences were not compromised. Furthermore, we are the first to report the migration velocity of MSC among which 30% were moving and velocity of about 15 mm h À1 was not altered by LV transduction. Manipulated MSC underwent senescence one passage earlier than non-transduced cells, suggesting the use for therapeutic intervention in early passage numbers. Upon tail vein application in nude mice, the majority of LV-transduced MSC could be detected in human orthotopic pancreatic tumor xenografts and to a minor extent in mouse liver, kidney and lung. Together, LV transduction of genes to MSC followed by puromycin selection is a powerful tool for basic research and improves the therapeutic prospects of MSC as vehicles in gene therapy.

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Section: Discussionsupporting

confidence: 55%

Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer

et al. 2008

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“…Messenger RNA for PAI-1 has been localised by in situ hybridization to endothelial cells within colon tumours [24]. It is interesting to note that MSCs have been reported to differentiate into endothelial cells and myofibroblasts [25,26].…”

Section: Discussionmentioning

confidence: 99%

Impact of Mesenchymal Stem Cell secreted PAI-1 on colon cancer cell migration and proliferation

Hogan

Joyce

Murphy

et al. 2013

Biochemical and Biophysical Research Communications

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“…As described previously (Oswald et al 2004), the BMSCs of NG group and SMG 72 h group were cultivated in DMEM, supplemented with 10 % FBS and 100 ng/mL vascular endothelial growth factor (VEGF, Invitrogen), 50 ng/mL epidermal growth factor (Invitrogen) and 1 lg/mL hydrocortisone (Sigma-Aldrich) for 14 days, aiming to induce endothelial differentiation. An inverted phase-contrast microscope (Eclipse TE 300; Nikon Co., Tokyo, Japan) was subsequently is 40 lm.…”

Section: Endothelium Oriented Differentiation Of Bmscsmentioning

confidence: 99%

“…Relatively easy to separate and culture is one of the main advantages of bone marrow mesenchymal stem cell (BMSC). In some specific conditions, BMSCs can differentiate into several distinct cell types, including chondrocytes, osteoblasts, smooth muscle cells, adipocytes, cardiac myocytes, endothelial cells and neurons (Pittenger et al 1999;Oswald et al 2004;Kodama et al 2005Kodama et al , 2006Tamama et al 2006Tamama et al , 2008Owen and Friedenstein 1988). Due to BMSC's capabilities in regenerating tissue, self-renewal and multipotential differentiation, it is highly desirable to use them in therapeutic renewal methods following aging, disease or trauma.…”

Section: Introductionmentioning

confidence: 99%

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Wang

Zhang

et al. 2013

Cytotechnology

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Multi-differentiation capability is an essential characteristic of bone marrow mesenchymal stem cells (BMSCs). Method on obtaining higherquality stem cells with an improved differentiation potential has gained significant attention for the treatment of clinical diseases and developmental biology. In our study, we investigated the multipotential differentiation capacity of BMSCs under simulated microgravity (SMG) condition. F-actin staining found that cytoskeleton took on a time-dependent change under SMG condition, which caused spindle to round morphological change of the cultured cells. Quantitative PCR and Western Blotting showed the pluripotency marker OCT4 was up-regulated in the SMG condition especially after SMG of 72 h, which we observed would be the most appropriate SMG duration for enhancing pluripotency of BMSCs. After dividing BMSCs into normal gravity (NG) group and SMG group, we induced them respectively in endothelium oriented, adipogenic and neuronal induction media. Immunostaining and Western Blotting found that endothelium oriented differentiated BMSCs expressed higher VWF and CD31 in the SMG group than in the NG group. The neuron-like cells derived from BMSCs in the SMG group also expressed higher level of MAP2 and NF-H. Furthermore, the quantity of induced adipocytes increased in the SMG group compared to the NG group shown by Oil Red O staining, The expression of PPARc2 increased significantly under SMG condition. Therefore, we demonstrated that SMG could promote BMSCs to differentiate into many kinds of cells and predicted that enhanced multi-potential differentiation capacity response in BMSCs following Electronic supplementary material The online version of this article

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Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro

Abstract: ABSTRACT

Cited by 1,165 publications

References 35 publications

Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer

Improved lentiviral transduction of human mesenchymal stem cells for therapeutic intervention in pancreatic cancer

Impact of Mesenchymal Stem Cell secreted PAI-1 on colon cancer cell migration and proliferation

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

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