Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis

Pan, Hong; Lam, Ping Keun; Tong, See Wai; Leung, Kevin Kai Chung; Teoh, Anthony Y.; Ng, Enders Kwok Wai

doi:10.1155/2020/5142797

Cited by 11 publications

(11 citation statements)

References 43 publications

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“…Although it has been demonstrated that endogenous MSCs contribute to tissue repair, their quantity and potency are insufficient to completely regenerate damaged tissue 14 . On the other hand, it has been proposed that transplanted MSCs are able to migrate to sites of damage and then differentiate into target cells and replace damaged tissues 15 . Therefore, researchers have been working to find exogenous MSC populations with favorable potential for migration, proliferation, and chondrogenic differentiation for cartilage regeneration.…”

Section: Role Of Mscs In Cartilage Repairmentioning

confidence: 99%

Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application

et al. 2021

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Articular cartilage is susceptible to damage but hard to self-repair due to its avascular nature. Traditional treatment methods are not able to produce satisfactory effects. Mesenchymal stem cells (MSCs) have shown great promise in cartilage repair. However, the therapeutic effect of MSCs is often unstable partly due to their heterogeneity. Understanding the heterogeneity of MSCs and the potential of different types of MSCs for cartilage regeneration will facilitate the selection of superior MSCs for treating cartilage damage. This review provides an overview of the heterogeneity of MSCs at the donor, tissue source and cell immunophenotype levels, including their cytological properties, such as their ability for proliferation, chondrogenic differentiation and immunoregulation, as well as their current applications in cartilage regeneration. This information will improve the precision of MSC-based therapeutic strategies, thus maximizing the efficiency of articular cartilage repair.

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Section: Role Of Mscs In Cartilage Repairmentioning

confidence: 99%

Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application

et al. 2021

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“…Pan et al [ 47 ] combined tissue fusion technology with allogenic ASCs in their 2020 publication. Sixteen pigs were divided in two groups related to the sacrifice date (7 or 14 d) and each group was subdivided in an ASC-treated or a control subgroup ( n = 4 each).…”

Section: Resultsmentioning

confidence: 99%

“…Clinically, five studies[ 40 , 44 , 45 , 49 , 51 ] reported significant lower AL incidence, five[ 32 , 40 , 45 , 49 , 50 ] reported fewer adhesions, four[ 40 , 45 , 49 , 51 ] fewer abscesses and one less mortality[ 44 ]. Eight studies[ 37 , 39 , 45 , 47 - 51 ], analyzed SC labelling and confirmed SC survival in this potentially septic area.…”

Section: Resultsmentioning

confidence: 99%

Stem cell therapy applied for digestive anastomosis: Current state and future perspectives

Trébol¹,

Georgiev-Hristov²,

Migueláñez³

et al. 2022

WJSC

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BACKGROUND Digestive tract resections are usually followed by an anastomosis. Anastomotic leakage, normally due to failed healing, is the most feared complication in digestive surgery because it is associated with high morbidity and mortality. Despite technical and technological advances and focused research, its rates have remained almost unchanged the last decades. In the last two decades, stem cells (SCs) have been shown to enhance healing in animal and human studies; hence, SCs have emerged since 2008 as an alternative to improve anastomoses outcomes. AIM To summarise the published knowledge of SC utilisation as a preventative tool for hollow digestive viscera anastomotic or suture leaks. METHODS PubMed, Science Direct, Scopus and Cochrane searches were performed using the key words “anastomosis”, “colorectal/colonic anastomoses”, “anastomotic leak”, “stem cells”, “progenitor cells”, “cellular therapy” and “cell therapy” in order to identify relevant articles published in English and Spanish during the years of 2000 to 2021. Studies employing SCs, performing digestive anastomoses in hollow viscera or digestive perforation sutures and monitoring healing were finally included. Reference lists from the selected articles were reviewed to identify additional pertinent articles. Given the great variability in the study designs, anastomotic models, interventions (SCs, doses and vehicles) and outcome measures, performing a reliable meta-analysis was considered impossible, so we present the studies, their results and limitations. RESULTS Eighteen preclinical studies and three review papers were identified; no clinical studies have been published and there are no registered clinical trials. Experimental studies, mainly in rat and porcine models and occasionally in very adverse conditions such as ischaemia or colitis, have been demonstrated SCs as safe and have shown some encouraging morphological, functional and even clinical results. Mesenchymal SCs are mostly employed, and delivery routes are mainly local injections and cell sheets followed by biosutures (sutures coated by SCs) or purely topical. As potential weaknesses, animal models need to be improved to make them more comparable and equivalent to clinical practice, and the SC isolation processes need to be standardised. There is notable heterogeneity in the studies, making them difficult to compare. Further investigations are needed to establish the indications, the administration system, potential adjuvants, the final efficacy and to confirm safety and exclude definitively oncological concerns. CONCLUSION The future role of SC therapy to induce healing processes in digestive anastomoses/sutures still needs to be determined and seems to be currently far from clinical use.

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“…Specifically, PCNA participates in cell proliferation by mediating DNA polymerase while blocking the PCNA production in cells severely affects cell division, which takes a significant part in the synthesis of DNA and its repair, cell proliferation, and progression of the cell cycle (77,78). Studies show treatment of MSCs could elevate the expression of PCNA, promote wound healing, and enhance re-epithelialization (79)(80)(81). Animals treated with MSCs show improving wound healing with no detectable side effects related to increasing viability, proliferation, and migration of epithelial cells (82).…”

Section: Mscs and Proliferation/migrationmentioning

confidence: 99%

3D bioprinted mesenchymal stromal cells in skin wound repair

Luo

et al. 2022

Front. Surg.

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Skin tissue regeneration and repair is a complex process involving multiple cell types, and current therapies are limited to promoting skin wound healing. Mesenchymal stromal cells (MSCs) have been proven to enhance skin tissue repair through their multidifferentiation and paracrine effects. However, there are still difficulties, such as the limited proliferative potential and the biological processes that need to be strengthened for MSCs in wound healing. Recently, three-dimensional (3D) bioprinting has been applied as a promising technology for tissue regeneration. 3D-bioprinted MSCs could maintain a better cell ability for proliferation and expression of biological factors to promote skin wound healing. It has been reported that 3D-bioprinted MSCs could enhance skin tissue repair through anti-inflammatory, cell proliferation and migration, angiogenesis, and extracellular matrix remodeling. In this review, we will discuss the progress on the effect of MSCs and 3D bioprinting on the treatment of skin tissue regeneration, as well as the perspective and limitations of current research.

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Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis

Cited by 11 publications

References 43 publications

Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application

Heterogeneity of mesenchymal stem cells in cartilage regeneration: from characterization to application

Stem cell therapy applied for digestive anastomosis: Current state and future perspectives

3D bioprinted mesenchymal stromal cells in skin wound repair

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