Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren’s Syndrome-Like Disease

Abughanam, Ghada; Elkashty, Osama A.; Liu, Younan; Bakkar, Mohammed; Tran, Simon D.

doi:10.3390/ijms20194750

Cited by 47 publications

(50 citation statements)

References 116 publications

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“…AQP5 has been used as a salivary acinar cell marker and displayed elevated gene expression in SGs of NOD mice treated with either spleen or mesenchymal stem cells [ 175 , 176 ]. Elevated gene expression of AQP1, AQP4 and AQP5 have been measured in mice models of xerostomia treated with a cell extract from either bone marrow or mesenchymal stem cells, which reduced salivary focus score, and restored saliva and tear flow rates [ 177 , 178 , 179 ].…”

Section: Therapeutic Strategies Aiming At Aqps To Treat Xerostomiamentioning

confidence: 99%

Insight into Salivary Gland Aquaporins

D’Agostino

Elkashty

Chivasso

et al. 2020

Cells

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The main role of salivary glands (SG) is the production and secretion of saliva, in which aquaporins (AQPs) play a key role by ensuring water flow. The AQPs are transmembrane channel proteins permeable to water to allow water transport across cell membranes according to osmotic gradient. This review gives an insight into SG AQPs. Indeed, it gives a summary of the expression and localization of AQPs in adult human, rat and mouse SG, as well as of their physiological role in SG function. Furthermore, the review provides a comprehensive view of the involvement of AQPs in pathological conditions affecting SG, including Sjögren’s syndrome, diabetes, agedness, head and neck cancer radiotherapy and SG cancer. These conditions are characterized by salivary hypofunction resulting in xerostomia. A specific focus is given on current and future therapeutic strategies aiming at AQPs to treat xerostomia. A deeper understanding of the AQPs involvement in molecular mechanisms of saliva secretion and diseases offered new avenues for therapeutic approaches, including drugs, gene therapy and tissue engineering. As such, AQP5 represents a potential therapeutic target in different strategies for the treatment of xerostomia.

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Section: Therapeutic Strategies Aiming At Aqps To Treat Xerostomiamentioning

confidence: 99%

Insight into Salivary Gland Aquaporins

D’Agostino

Elkashty

Chivasso

et al. 2020

Cells

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“…These findings showed that transplanted MSCs may have exerted their regenerative effect either via differentiation of MSCs to SGs epithelial cells or promotion of stem cell of submandibular gland. The expression of Ki-67 as one of the several salivary and lacrimal glands markers involved in regeneration and proliferation has been recently demonstrated by Abughanam et al. (2019) .…”

Section: Discussionmentioning

confidence: 99%

Reconstruction of necrotic submandibular salivary gland using mesenchymal stem cells

Najafi

Hassan

Faraji

et al. 2020

Heliyon

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Background The efficacy of mesnchymal stem cells (MSCs) to treat the necrotic tissue of salivary glands (SGs) has yet investigated. Objective This study was conducted to investigate the potential capacity of MSCs to restore the function and regenerate the necrotic submandiular gland in the rat animal model. Methods Twenty-one Sprague–Dawley rats were provided from a breeding colony and randomly divided into three groups including the positive control or induced SG atrophy without treatment, the treatment group or induced SG atrophy with MSCs isolated transplantation and the negative control group consists of healthy rats. The atrophic and necrotic submandiular gland was induced using intraoral duct ligation of the main duct of submandiular gland for one month. The isolated stem cells were confirmed using flow cytometry for CD90 and CD 105. The isolated MSCs were cultured and injected to submandiular gland and the potential efficacy of MSCs to treat the atrophic submandibular glands was evaluated using histopathology on two weeks post-transplantation. To detect the acinar cell protein secretory granules, Alcian Blue and periodic acid shift (PAS) staining were done. For the demonstration of mitotic index or proliferation rate of the SG epithelia tissue, Ki-67 and Smbg proteins expression were evaluated using immunohistochemistry. Results The locally injected MSCs could regenerate the overall histological structure of the necrotic submandibular gland tissue within 2 weeks of post-transplantation. Alcian Blue and PAS staining indicated that the mean amount of serous and mucin secretions in the treatment group was significantly increased compared to the positive control groups. We have also found that the treatment group significantly express higher Ki-67 protein, as a diagnostic marker for cell mitosis and proliferation rate, and lower Smbg protein, as a diagnostic marker, for damage to the submandibular gland than that of control group. Conclusion This study demonstrates the therapeutic benefits of MSCs isolated from the SG in treating atrophic and necrotic SGs in a rat model. MSCs may be potential candidates for cell-based therapies targeting hypofunction of SG induced by a range of diseases or because of surgery and radiotherapy of head and neck cancers.

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“…Mesenchymal stem cells (MSCs) are multipotent stromal cells that possess self-renewing capacity and give rise to many specialized cell types such as chondrocytes, adipocytes, myocytes, and osteoblasts. MSCs are primarily located within the bone marrow (BM) stroma, but they can be isolated from almost any organ [ 86 , 87 , 88 ]. MVs produced by horse amniotic MSCs reduced the secretion of TNF-α and, to a certain extent, TGF-β and IL-6 from primary equine alveolar macrophages [ 89 ].…”

Section: Extracellular Vesicles and Asthma: Cellular Levelmentioning

confidence: 99%

Extracellular Vesicles and Asthma—More Than Just a Co-Existence

Alhamwe

Potaczek

Miethe

et al. 2021

IJMS

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Extracellular vesicles (EVs) are membranous structures, which are secreted by almost every cell type analyzed so far. In addition to their importance for cell-cell communication under physiological conditions, EVs are also released during pathogenesis and mechanistically contribute to this process. Here we summarize their functional relevance in asthma, one of the most common chronic non-communicable diseases. Asthma is a complex persistent inflammatory disorder of the airways characterized by reversible airflow obstruction and, from a long-term perspective, airway remodeling. Overall, mechanistic studies summarized here indicate the importance of different subtypes of EVs and their variable cargoes in the functioning of the pathways underlying asthma, and show some interesting potential for the development of future therapeutic interventions. Association studies in turn demonstrate a good diagnostic potential of EVs in asthma.

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Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren’s Syndrome-Like Disease

Cited by 47 publications

References 116 publications

Insight into Salivary Gland Aquaporins

Insight into Salivary Gland Aquaporins

Reconstruction of necrotic submandibular salivary gland using mesenchymal stem cells

Extracellular Vesicles and Asthma—More Than Just a Co-Existence

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