Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair

Krampera, Mauro; Pizzolo, Giovanni; Aprili, Giuseppe; Franchini, Massimo

doi:10.1016/j.bone.2006.04.020

Cited by 293 publications

(211 citation statements)

References 96 publications

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“…Por su capacidad de diferenciación a múltiples linajes, específicamente por su potencialidad osteogénica y sus propiedades inmunomoduladoras, antiinflamatorias y anti-apoptóticas, las células madre mesenquimales se han convertido en la principal herramienta de la terapia celular para el tratamiento regenerativo de enfermedades que afectan funcionalmente al tejido óseo (1,3,8,24,(55)(56)(57).…”

Section: Discussionunclassified

“…Las células madre mesenquimales son células adultas multipotentes, capaces de autorrenovarse y diferenciarse a linajes mesenquimales como el osteogénico, condrogénico, adipogénico y miogénico, aunque también se ha demostrado su capacidad de diferenciarse a células de origen no mesenquimal como las del tejido nervioso (5)(6)(7)(8).…”

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“…Por todo esto, las células madre mesenquimales son hoy en día el centro de múltiples estudios clínicos para ciertas entidades como infarto del miocardio, diabetes, alteraciones cerebrales y de médula espinal, enfermedades cardiovasculares, enfermedad de injerto contra huésped, enfermedad de Crohn (1,6) y otras que involucran el tejido óseo y cartilaginoso (8,12,(23)(24)(25).…”

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Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano

2013

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Biomédica 2014;34:67-78 Ad-MSC en hidrogeles de plasma sanguíneo humano Contribución de los autores:Itali M. Linero: diseño de experimentos, obtención, cultivo y caracterización de las Ad-MSC, elaboración del hidrogel de plasma sanguíneo humano, escritura y revisión del manuscrito. Adriana Doncel: obtención, cultivo y caracterización de las Ad-MSC, y revisión del manuscrito. Orlando Chaparro: diseño de experimentos, escritura y revisión del manuscrito. Introducción. La utilización de las células madre mesenquimales en la práctica clínica ha aumentado considerablemente en la última década, ya que juegan un papel favorable en los procesos de reparación y regeneración tisular, siendo la principal herramienta de la terapia celular para el tratamiento de enfermedades que afectan funcionalmente el tejido óseo y cartilaginoso. Objetivo. Evaluar la proliferación y capacidad de diferenciación osteogénica in vitro de células madre mesenquimales derivadas de tejido adiposo humano en un hidrogel de plasma sanguíneo. Materiales y métodos. Se obtuvieron células madre mesenquimales a partir de explantes de tejido adiposo humano y se caracterizaron por citometría de flujo; se buscó demostrar su multipotencialidad por su capacidad de diferenciación osteogénica y adipogénica. Se evaluó la proliferación celular y la capacidad de diferenciación osteogénica de las células cultivadas en hidrogeles de plasma sanguíneo.Resultados. Las células madre mesenquimales derivadas de tejido adiposo cultivadas en el hidrogel de plasma sanguíneo humano mostraron un patrón de proliferación muy similar al de las células cultivadas en monocapa y, además, mantuvieron su capacidad de diferenciación hacia el linaje osteogénico. Conclusiones. El hidrogel de plasma sanguíneo humano es un soporte adecuado para que las células madre mesenquimales derivadas de tejido adiposo humano proliferen y se diferencien hacia el linaje osteogénico y constituye un vehículo adecuado para su administración en regeneración del tejido óseo.Palabras clave: células madre, hidrogeles, plasma, regeneración ósea.doi: http://dx.doi.org/10.7705/biomedica.v34i1.1465 Proliferation and osteogenic differentiation of mesenchymal stem cells in hydrogels of human blood plasmaIntroduction: The use of mesenchymal stem cells in clinical practice has increased considerably in the last decade because they play a supporting role in the processes of tissue repair and regeneration, becoming the main tool of cell therapy for the treatment of diseases functionally affecting bone and cartilage tissue. Objective: To evaluate in vitro the proliferative and osteogenic differentiation ability of mesenchymal stem cells derived from human adipose tissue in a blood plasma hydrogel. Materials and methods: Mesenchymal stem cells were obtained from human adipose tissue explants and characterized by flow cytometry. Their multipotentiality was demonstrated by their ability to differentiate to adipogenic and osteogenic lineages. Cell proliferation and osteogenic differentiation ability of the cells cultured...

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Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano

2013

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“…Under appropriate culture conditions, MSC are capable of differentiating towards the osteogenic, chondrogenic, myogenic and adipogenic lineages (Barry et al, 2001;Franchini, 2003;Krampera et al, 2006;Pittenger et al, 1999). Bone marrow-derived MSC (BMSC) represent the most intensively investigated MSC type in cartilage tissue engineering.…”

Section: The Potential Of Msc To Improve the State Of The Artmentioning

confidence: 99%

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

Hildner

Albrecht

Gabriel

et al. 2011

J Tissue Eng Regen Med

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Trauma, malposition and age-related degeneration of articular cartilage often result in severe lesions that do not heal spontaneously. Many efforts over the last centuries have been undertaken to support cartilage healing, with approaches ranging from symptomatic treatment to structural cartilage regeneration. Microfracture and matrix-associated autologous chondrocyte transplantation (MACT) can be regarded as one of the most effective techniques available today to treat traumatic cartilage defects. Research is focused on the development of new biomaterials, which are intended to provide optimized physical and biochemical conditions for cell proliferation and cartilage synthesis. New attempts have also been undertaken to replace chondrocytes with cells that are more easily available and cause less donor site morbidity, e.g. adipose derived stem cells (ASC). The number of in vitro studies on adult stem cells has rapidly increased during the last decade, indicating that many variables have yet to be optimized to direct stem cells towards the desired lineage. The present review gives an overview of the difficulties of cartilage repair and current cartilage repair techniques. Moreover, it reviews new fields of cartilage tissue engineering, including stem cells, co-cultures and platelet-rich plasma (PRP).

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“…19,20 MSCs can differentiate into different cells with ectodermal and endodermal characteristics, suggesting the possibility of MSCs for cell replacement and tissue regeneration. [21][22][23][24][25] The previous MSC research has focused on the effects of soluble cues, such as growth factors and cytokines. 26 However, there are increasing interests on understanding how extracellular matrix (ECM) components influence MSC behaviour and properties.…”

Section: Introductionmentioning

confidence: 99%

Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study

Liu

Liang

et al. 2012

Biomicrofluidics

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Precisely controlling the spatial distribution of biomolecules on biomaterial surface is important for directing cellular activities in the controlled cell microenvironment. This paper describes a polydimethylsiloxane (PDMS) gradient-generating microfluidic device to immobilize the gradient of cellular adhesive Arg-Gly-Asp (RGD) peptide on poly (ethylene glycol) (PEG) hydrogel. Hydrogels are formed by exposing the mixture of PEG diacrylate (PEGDA), acryloyl-PEG-RGD, and photo-initiator with ultraviolet light. The microfluidic chip was simulated by a fluid dynamic model for the biomolecule diffusion process and gradient generation. PEG hydrogel covalently immobilized with RGD peptide gradient was fabricated in this microfluidic device by photo-polymerization. Bone marrow derived rat mesenchymal stem cells (MSCs) were then cultured on the surface of RGD gradient PEG hydrogel. Cell adhesion of rat MSCs on PEG hydrogel with various RGD gradients were then qualitatively and quantitatively analyzed by immunostaining method. MSCs cultured on PEG hydrogel surface with RGD gradient showed a grated fashion for cell adhesion and spreading that was proportional to RGD concentration. It was also found that 0.107-0.143 mM was the critical RGD concentration range for MSCs maximum adhesion on PEG hydrogel.

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Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair

Cited by 293 publications

References 96 publications

Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano

Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

Covalently immobilized biomolecule gradient on hydrogel surface using a gradient generating microfluidic device for a quantitative mesenchymal stem cell study

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