Mesenchymal Stromal Cell Proliferation, Gene Expression and Protein Production in Human Platelet-Rich Plasma-Supplemented Media

Amable, Paola Romina; Teixeira, Marcus Vinicius Telles; Carias, Rosana Bizon Vieira; Granjeiro, José Mauro; Borojević, Radovan

doi:10.1371/journal.pone.0104662

Cited by 78 publications

(121 citation statements)

References 27 publications

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“…Our data corroborate the previous observations on detection of the matrix metalloproteinases in conditioned medium of actively self-renewing hADSCs [39,40] and further suggests a caution against including MMP1, MMP3, MMP9 and MMP13 as unique components of SMS or SASP.…”

Section: Matrix Metalloproteinases Do Not Comprise Unique Compositionsupporting

confidence: 91%

“…During development angiogenesis and adipogenesis are strongly related, and it comes as no surprise that hADSCs secrete varieties of angiogenic factors [7]. Essential parts of [39,40], which degrade several ECM proteins, including fibronectin, collagen and elastin. MMP3 can also participate in activation of MMP1 and MMP9.…”

Section: Matrix Metalloproteinases Do Not Comprise Unique Compositionmentioning

confidence: 99%

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Acute Genotoxic Stress-Induced Senescence in Human Mesenchymal Cells Drives a Unique Composition of Senescence Messaging Secretome (SMS)

Gaur¹,

Wang²,

Àlex³

et al. 2017

J Stem Cell Res Ther

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Section: Matrix Metalloproteinases Do Not Comprise Unique Compositionsupporting

confidence: 91%

Section: Matrix Metalloproteinases Do Not Comprise Unique Compositionmentioning

confidence: 99%

Acute Genotoxic Stress-Induced Senescence in Human Mesenchymal Cells Drives a Unique Composition of Senescence Messaging Secretome (SMS)

Gaur¹,

Wang²,

Àlex³

et al. 2017

J Stem Cell Res Ther

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“…These cultured ADSCs also maintained their differentiation potential, and were able to differentiate into adipocytes and osteoblasts… With these phenotypes, obtained ADSCs satisfied the minimal standard depicted by Dominici et al (Dominici et al, 2006). Similar studies have been successful in replacing FBS with PRP in MSC culture conditions, including those from as bone marrow (Amable et al, 2014;Zou et al, 2014), and umbilical cord blood Van Pham and Kim Phan, 2014).…”

Section: Discussionmentioning

confidence: 74%

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Pham¹,

Vu²,

Le³

et al. 2014

Biomed Res Ther

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Abstract-Adipose-derived stem cells (ADSCs) are excellent for regenerative medicine. Like mesenchymal stem cells, ADSCs possess multi-potent differentiation capacity that enables them to differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into other cells. ADSC transplantation has gained attention in recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 15th passage could not form tumors in NOD/SCID mice. This research produced a suitable protocol for clinical applications of expanded ADSCs.

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“…ERK-signaling inhibits MSC differentiation by blocking PPARγ and CEBPα expression. In contrast to the above-quoted studies, Amable et al [43] described a reduced adipogenic differentiation capacity of ASCs cultured with PRP associated with PPARγ downregulation.…”

Section: Prp and Pl As An Autologous Cell Culture Supplementmentioning

confidence: 85%

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

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Mesenchymal Stromal Cell Proliferation, Gene Expression and Protein Production in Human Platelet-Rich Plasma-Supplemented Media

Cited by 78 publications

References 27 publications

Acute Genotoxic Stress-Induced Senescence in Human Mesenchymal Cells Drives a Unique Composition of Senescence Messaging Secretome (SMS)

Acute Genotoxic Stress-Induced Senescence in Human Mesenchymal Cells Drives a Unique Composition of Senescence Messaging Secretome (SMS)

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

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