Mesoporous Silica Nanoparticles: A Potential Platform for Generation of Induced Pluripotent Stem Cells?

Chiou, Shih Hwa; Jang, Shih Fan; Mou, Chung‐Yuan

doi:10.2217/nnm.14.9

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…8 Mou and co-workers designed and synthesized a series of cationic surfactanttemplated MSNs for intracellular drug delivery and bio-imaging. [9][10][11] Yu and co-workers presented the synthesis of silica ellipsoids with a hexagonal mesostructure via an organic-inorganic cooperative assembly process by using the amphiphilic copolymer P123 as a template. 12 Che and coworkers introduced co-structure directing agents to synthesize anionic surfactant-templated silica nanoparticles with highly ordered structures.…”

Section: Introductionmentioning

confidence: 99%

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe³⁺

et al. 2016

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This work demonstrates a novel method for the synthesis of large pore mesoporous silica nanoparticles (MSNs) with a pore diameter of 10.3 nm and a particle diameter of ∼50 nm based on the incorporation of mixed anionic surfactants sodium dodecyl benzene sulfonate (SDBS) and sodium dodecyl sulphate (SDS) as the template in the synthesis process. The dispersity, morphology, pore structure and size of mesoporous nanoparticles were adjusted by changing the molar ratio of two anionic surfactants, the concentration of the co-structure-directing agent (3-aminopropyltrimethoxysilane) and the reaction temperature. The results of synthesis experiments suggested that the formation of large pore MSNs involved a nucleation and growth process. MSNs were post-grafted with a Schiff base moiety for fluorescence sensing of Fe(3+) in water. The applicability of functionalized MSNs was demonstrated by selective fluorescence detection of Fe(3+) in aqueous media.

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Section: Introductionmentioning

confidence: 99%

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe³⁺

et al. 2016

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“…Induced pluripotent stem cells (iPSCs) was first generated in 2006 when Yamanaka and Takahashi used a defined set of reprogramming factors: Oct4, Sox2, Klf4, and c-Myc (OSKM), which are commonly referred to as the “Yamanaka factors”, , to reprogram mouse embryonic fibroblast into a pluripotent state. Later, intense research provided the evidence that iPSCs could be derived from different species (human included) using various combinations from the transcription factors such as Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28 .…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

Deng

Cao

Chen

et al. 2015

ACS Appl. Mater. Interfaces

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Induced pluripotent stem cells (iPSCs), resulting from the forced expression of cocktails out of transcription factors, such as Oct4, Sox2, Klf4, and c-Myc (OSKM), has shown tremendous potential in regenerative medicine. Although rapid progress has been made recently in the generation of iPSCs, the safety and efficiency remain key issues for further application. In this work, microRNA 302-367 was employed to substitute the oncogenic Klf4 and c-Myc in the OSKM combination as a safer strategy for successful iPSCs generation. The negatively charged plasmid mixture (encoding Oct4, Sox2, miR302-367) and the positively charged cationized Pleurotus eryngii polysaccharide (CPEPS) self-assembled into nanosized particles, named as CPEPS-OS-miR nanoparticles, which were applied to human umbilical cord mesenchymal stem cells for iPSCs generation after characterization of the physicochemical properties. The CPEPS-OS-miR nanoparticles possessed spherical shape, ultrasmall particle size, and positive surface charge. Importantly, the combination of plasmids Oct4, Sox2, and miR302-367 could not only minimize genetic modification but also show a more than 50 times higher reprogramming efficiency (0.044%) than any other single or possible double combinations of these factors (Oct4, Sox2, miR302-367). Altogether, the current study offers a simple, safe, and effective self-assembly approach for generating clinically applicable iPSCs.

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“…Nanoparticles emerged as a novel tool for transgene delivery to iPSCs. Mesoporous silica nanoparticles (MSNs) were utilized and evaluated for the generation of iPSCs [104,105].…”

Section: Nanoparticles In Ipsc Generation and Precision Medicinementioning

confidence: 99%

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

2021

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Human induced pluripotent stem cells (HiPSCs) and HiPSCs-derived cells have the potential to revolutionize regenerative and precision medicine. Genetically reprograming somatic cells to generate HiPSCs and genetic modification of HiPSCs are considered the key procedures for the study and application of HiPSCs. However, there are significant technical challenges for transgene delivery into somatic cells and HiPSCs since these cells are known to be difficult to transfect. The existing methods, such as viral transduction and chemical transfection, may introduce significant alternations to hiPSC culture which affect the potency, purity, consistency, safety, and functional capacity of HiPSCs. Therefore, generation and genetic modification of HiPSCs through non-viral approaches are necessary and desirable. Nanotechnology has revolutionized fields from astrophysics to biology over the past two decades. Increasingly, nanoparticles have been used in biomedicine as powerful tools for transgene and drug delivery, imaging, diagnostics, and therapeutics. The most successful example is the recent development of SARS-CoV-2 vaccines at warp speed to combat the 2019 coronavirus disease (COVID-19), which brought nanoparticles to the center stage of biomedicine and demonstrated the efficient nanoparticle-mediated transgene delivery into human body. Nanoparticles have the potential to facilitate the transgene delivery into the HiPSCs and offer a simple and robust approach. Nanoparticle-mediated transgene delivery has significant advantages over other methods, such as high efficiency, low cytotoxicity, biodegradability, low cost, directional and distal controllability, efficient in vivo applications, and lack of immune responses. Our recent study using magnetic nanoparticles for transfection of HiPSCs provided an example of the successful applications, supporting the potential roles of nanoparticles in hiPSC biology. This review discusses the principle, applications, and significance of nanoparticles in the transgene delivery to HiPSCs and their successful application in the development of COVID-19 vaccines.

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Mesoporous Silica Nanoparticles: A Potential Platform for Generation of Induced Pluripotent Stem Cells?

Cited by 6 publications

References 25 publications

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe³⁺

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe³⁺

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

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Mesoporous Silica Nanoparticles: A Potential Platform for Generation of Induced Pluripotent Stem Cells?

Cited by 6 publications

References 25 publications

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe3+

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe3+

MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via CationizedPleurotus eryngiiPolysaccharide-based Nanotransfection

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

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Product

Resources

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Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe³⁺

Mixed anionic surfactant-templated mesoporous silica nanoparticles for fluorescence detection of Fe³⁺