Mesoporous Silica Nanoparticles for Combined Delivery of Polo-Like Kinase 1 (PLK1) and Epidermal Growth Factor Receptor (EGFR) Inhibitors Enhances Radio Sensibility in Non-Small Cell Lung Cancer

Li, Changbo; Chen, Zelan; Li, Xingming; Xiao, Huayi; Liu, Qiang; Zhao, Liang; Li, Xia; Xian, Feng

doi:10.1166/sam.2021.4097

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Different types of nanoparticles have been widely used in cancer detection and diagnosis, such as metal [ 7 ], non-metal [ 8 ], polymer [ 9 ], etc. Nanodiamond (ND) is a carbon-based nanomaterial for bio-imaging and bio-sensing [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Lin

Raj

et al. 2022

Pharmaceutics

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Nanoprobes provide advantages for real-time monitoring of tumor markers and tumorigenesis during cancer progression and development. Epidermal growth factor receptor (EGFR) is a key protein that plays crucial roles for tumorigenesis and cancer therapy of lung cancers. Here, we show a carbon-based nanoprobe, nanodiamond (ND), which can be applied for targeting EGFR and monitoring tumorigenesis of human lung cancer cells in vitro and in vivo. The optimal fluorescent intensities of ND particles were observed in the human lung cancer cells and nude mice under in vivo imaging system. The fluorescence signal of ND particles can be real-time detected in the xenografted human lung tumor formation of nude mice. Moreover, the ND-conjugated specific EGFR antibody cetuximab (Cet) can track the location and distribution of EGFR proteins of lung cancer cells in vitro and in vivo. ND-Cet treatment increased cellular uptake ability of nanocomposites in the EGFR-expressed cells but not in the EGFR-negative lung cancer cells. Interestingly, single ND-Cet complex can be directly observed on the protein G bead by immunoprecipitation and confocal microscopy. Besides, the EGFR proteins were transported to lysosomes for degradation. Together, this study demonstrates that ND-conjugated Cet can apply for targeting EGFR and monitoring tumorigenesis during lung cancer progression and therapy.

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Section: Introductionmentioning

confidence: 99%

Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Lin

Raj

et al. 2022

Pharmaceutics

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Effects of ROS-Sensitive Nanoparticles Loaded with Interstitial Cell Derived Factors on Vascular Smooth Cell Adhesion, Proliferation, and Endothelialization

Zhang

Ding

2023

sci adv mater

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Local high concentrations of stromal cell-derived factor 1 (SDF-1) attract and trap stem cells to involve in the vascular repair when the skin is damaged. In this research, mercaptan ketone polymer (PRGY) was selected as a nano-carrier to prepare ROS-sensitive nanoparticles (NPs) loaded with SDF-1 by means of volatilization of a complex emulsion solvent. The NPs were characterized by a transmission electron microscope (TEM) and a particle size (PS) analyzer, the protein content of the NPs was evaluated by a BCA method, the cytotoxicity was evaluated by CCK8, and the NPs were subjected to the in vitro release (IVR) test. An acute mouse wound model was established, the pathological effect of NP solution on major organs of mice was observed by HE staining after intravenous injection, and the effect of targeted release of NP on animal model was evaluated. To analyze the effect of NPs on wound healing, the mouse models were rolled into different groups. They were sacrificed two weeks after the wound healing was completed, the blood vessels were fixed with perfusion solution, and the common artery where the wound was located was taken for follow-up study. The results suggested that the NPs exhibited a PS of 122.57±18.33 nm, a loading rate of 1.7%, and spherical and uniform surfaces. The IVR tests showed that the NPs could release rapidly under high ROS conditions. The wound model of mice was established, and the injection of NP solution revealed that it could target the wound area. The healing speed in the NP group was the fastest (P <0.05). Smooth muscle cells (SMCs) and endothelial cells (ECs) were isolated from common artery, and proliferation of them in the NP group was found to be obvious (P <0.05), and the adhesion of vascular SMCs could be accelerated at the same time.

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Effects of Fluorouracil-Carrying Nano Targeted Liposomes on Proliferation, Migration, and Invasion of Gastric Cancer Cells

Yang¹

2023

sci adv mater

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Fluorouracil (FU) is a common chemotherapy drug. To overcome the shortcomings of the original drug, such as strong gastrointestinal reaction and short half-life, FU was placed in the Mahadevi layered complex (MLC) added with dextran (DEX) for surface modification to obtain the magnetic targeting nanoparticles (MLC-Dex-Fu) with sustained release. Next, the MLC-Dex-Fu was combined with liposome to obtain the FU-carrying nano targeted liposome (LMDF). MGC-803 gastric cancer (GC) cells were selected to establish the nude mouse model of GC solid tumor. The nude mice were rolled into different groups (a control group (Ctrl group), a FU group, a LMDF group without magnetic field, and a LMDF group with magnetic field). The diet and weight of nude mice were observed after interventions under the conditions of NMF and MF. The tumor tissues were removed, cleaned, cut into homogenate, transferred to the pre-cooled Eppendorf (EP) tube, and then performed with homogenation, suction by pipettes, and filtration to obtain the tumor cell suspensions of the corresponding groups. Partial tumor cell suspensions were based to analyze the cell proliferation by flow cytometry (FCT), and the other suspensions were based to investigate the migration and invasion by the Transwell method. The results suggested the cell viability in the LMDF groups was greatly decreased (P < 0.05). In the nude mouse model test, LMDF exhibited a good magnetic targeted transport and sustained release chemotherapy, and could guide FU to move in vivo and enrich in the tumor site with a high concentration under the effects of external magnetic field, thus inhibiting the tumor proliferation. Such efficacy was more obvious in comparison with other groups (P < 0.05).

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Mesoporous Silica Nanoparticles for Combined Delivery of Polo-Like Kinase 1 (PLK1) and Epidermal Growth Factor Receptor (EGFR) Inhibitors Enhances Radio Sensibility in Non-Small Cell Lung Cancer

Cited by 3 publications

References 25 publications

Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Targeting EGFR and Monitoring Tumorigenesis of Human Lung Cancer Cells In Vitro and In Vivo Using Nanodiamond-Conjugated Specific EGFR Antibody

Effects of ROS-Sensitive Nanoparticles Loaded with Interstitial Cell Derived Factors on Vascular Smooth Cell Adhesion, Proliferation, and Endothelialization

Effects of Fluorouracil-Carrying Nano Targeted Liposomes on Proliferation, Migration, and Invasion of Gastric Cancer Cells

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