Mesoporous silica nanoparticles for <sup>19</sup>F magnetic resonance imaging, fluorescence imaging, and drug delivery

Nakamura, Tatsuya; Sugihara, Fuminori; Matsushita, Hisashi; Yoshioka, Yoshichika; Mizukami, Shin; Kikuchi, Kazuya

doi:10.1039/c4sc03549f

Cited by 118 publications

(150 citation statements)

References 37 publications

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“…A complete list of peptides used in this study can be found in Figure S1 B) Disordered peptide design showing 19 F sources alternating with positive charge causing a random-coil through electrostatic repulsion. C) Select fluorinated molecules used for 19 F MRI: i) Perfluoro-15-crown-5 ether [6] ii) Perfluoro(polyethylene glycol) methyl ether [13] and iii) PERFECTA. [14] …”

Section: Figurementioning

confidence: 99%

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal ¹⁹F MRI Agents

et al. 2017

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19F MRI is valuable for in vivo imaging due to the only trace amounts of fluorine in biological systems. Because of the low sensitivity of MRI however, designing new fluorochemicals remains a significant challenge for achieving sufficient 19F signal. Here, we describe a new class of high-signal, water-soluble fluorochemicals as 19F MRI imaging agents. A polyamide backbone is used for tuning the proteolytic stability to avoid retention within the body, which is a limitation of current state-of-the-art perfluorochemicals. We show that unstructured peptides containing alternating N-ε-trifluoroacetyllysine and lysine provide a degenerate 19F NMR signal. 19F MRI phantom images provide sufficient contrast at micromolar concentrations, showing promise for eventual clinical applications. Finally, the degenerate high signal characteristics were retained when conjugated to a large protein, indicating potential for in vivo targeting applications, including molecular imaging and cell tracking.

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Section: Figurementioning

confidence: 99%

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal ¹⁹F MRI Agents

et al. 2017

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“…Nakamura et al . 38 reported a PFC core-shell MSN called mFLAME filled with PFCE for drug delivery. The mFLAME particles were modified with different surface ligands which allowed for dual mode detection using 19 F-MRI and fluorescence microscopy.…”

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confidence: 99%

Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles

et al. 2017

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Oxygen homeostasis is important in the regulation of biological function. Disease progression can be monitored by measuring oxygen levels, thus producing information for the design of therapeutic treatments. Non-invasive measurements of tissue oxygenation require the development of tools with minimal adverse effects and facile detection of features of interest. Fluorine magnetic resonance imaging (19F-MRI) exploits the intrinsic properties of perfluorocarbon (PFC) liquids for anatomical imaging, cell tracking, and oxygen sensing. However, the highly hydrophobic and lipophobic properties of perfluorocarbons require the formation of emulsions for biological studies. Though, stabilizing these emulsions has been challenging. To enhance the stability and biological loading of perfluorocarbons, one option is to incorporate perfluorocarbon liquids into the internal space of biocompatible mesoporous silica nanoparticles. Here, we developed perfluorocarbon-loaded ultraporous mesostructured silica nanoparticles (PERFUMNs) as 19F-MRI detectable oxygen sensing probes. Ultraporous mesostructured nanoparticles (UMNs) have large internal cavities (average = 1.76 cm3 g−1), facilitating an average 17% loading efficiency of PFCs, meeting the threshold fluorine concentrations needed for imaging studies. Perfluoro-15-crown-5-ether PERFUMNs have the highest equivalent nuclei per PFC molecule, and a spin-lattice (T1) relaxation-based oxygen sensitivity of 0.0032 mmHg−1 s−1 at 16.4 T (657 MHz). The option of loading PFCs after synthesizing UMNs, rather than the more traditional in situ core-shell syntheses, allows for use of a broad range of PFC liquids from a single material. The biocompatible and tunable chemistry of UMNs combined with the intrinsic properties of PFCs makes PERFUMNs a MRI sensor with potential for anatomical imaging, cell tracking, and metabolic spectroscopy with improved stability.

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“…To reduce often observed clustering and aggregating of the AuNRs without CTAB, the surface of AuNRs is often modified with mesoporous silica or all kinds of polymers [6,7]. And the large specific surface area of mesoporous silica and polymers guarantees the large loading capacity [8][9][10]. Nevertheless, the performance of these modified nanocapsules is not as good as initially envisioned.…”

Section: Introductionmentioning

confidence: 96%

Fabrication of superstable gold nanorod–carbon nanocapsule as a molecule loading material

et al. 2015

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Mesoporous silica nanoparticles for ¹⁹F magnetic resonance imaging, fluorescence imaging, and drug delivery

Abstract: We described perfluorocarbon encapsulated in mesoporous silica nanoparticles which enabled dual modal imaging (NIR/19F MRI) and drug delivery.

Cited by 118 publications

References 37 publications

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal ¹⁹F MRI Agents

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal ¹⁹F MRI Agents

Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles

Fabrication of superstable gold nanorod–carbon nanocapsule as a molecule loading material

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Mesoporous silica nanoparticles for 19F magnetic resonance imaging, fluorescence imaging, and drug delivery

Abstract: We described perfluorocarbon encapsulated in mesoporous silica nanoparticles which enabled dual modal imaging (NIR/19F MRI) and drug delivery.

Cited by 118 publications

References 37 publications

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal 19F MRI Agents

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal 19F MRI Agents

Oxygen Sensing with Perfluorocarbon-Loaded Ultraporous Mesostructured Silica Nanoparticles

Fabrication of superstable gold nanorod–carbon nanocapsule as a molecule loading material

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Product

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Mesoporous silica nanoparticles for ¹⁹F magnetic resonance imaging, fluorescence imaging, and drug delivery

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal ¹⁹F MRI Agents

Synthesis of Intrinsically Disordered Fluorinated Peptides for Modular Design of High‐Signal ¹⁹F MRI Agents