Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria

Zhang, Liyuan; Liang, Zhen; Yang, Kaiguang; Xia, Simin; Wu, Qi; Zhang, Lihua; Zhang, Yukui

doi:10.1016/j.aca.2012.04.005

Cited by 33 publications

(11 citation statements)

References 41 publications

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“…In the present study, we investigated the mechanism of brain neurotoxicity of nano-TiO 2 . We find that high doses of nano-TiO 2 can produce lipid peroxidation and decrease antioxidant capacity, including SOD, CAT, APX, and GSHPx activities, resulting in oxidative stress, which may damage unsaturated fatty acids and brain tissue [ 24 , 26 , 37 , 40 ]. We observed rupture and cracking in nerve cells and the infiltration of inflammatory cells in the brain.…”

Section: Discussionmentioning

confidence: 99%

The Potential Liver, Brain, and Embryo Toxicity of Titanium Dioxide Nanoparticles on Mice

et al. 2017

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Nanoscale titanium dioxide (nano-TiO2) has been widely used in industry and medicine. However, the safety of nano-TiO2 exposure remains unclear. In this study, we evaluated the liver, brain, and embryo toxicity and the underlying mechanism of nano-TiO2 using mice models. The results showed that titanium was distributed to and accumulated in the heart, brain, spleen, lung, and kidney of mice after intraperitoneal (i.p.) nano-TiO2 exposure, in a dose-dependent manner. The organ/body weight ratios of the heart, spleen, and kidney were significantly increased, and those of the brain and lung were decreased. High doses of nano-TiO2 significantly damaged the functions of liver and kidney and glucose and lipid metabolism, as showed in the blood biochemistry tests. Nano-TiO2 caused damages in mitochondria and apoptosis of hepatocytes, generation of reactive oxygen species, and expression disorders of protective genes in the liver of mice. We found ruptured and cracked nerve cells and inflammatory cell infiltration in the brain. We also found that the activities of constitutive nitric oxide synthases (cNOS), inducible NOS (iNOS), and acetylcholinesterase, and the levels of nitrous oxide and glutamic acid were changed in the brain after nano-TiO2 exposure. Ex vivo mouse embryo models exhibited developmental and genetic toxicity after high doses of nano-TiO2. The size of nano-TiO2 particles may affect toxicity, larger particles producing higher toxicity. In summary, nano-TiO2 exhibited toxicity in multiple organs in mice after exposure through i.p. injection and gavage. Our study may provide data for the assessment of the risk of nano-TiO2 exposure on human health.

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Section: Discussionmentioning

confidence: 99%

The Potential Liver, Brain, and Embryo Toxicity of Titanium Dioxide Nanoparticles on Mice

et al. 2017

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“…Authors successfully detected 304 phosphorylation sites containing 259 on serine, 39 on threonine and six on tyrosine at fmol concentration. Similarly, mesoporous TiO 2 aerogel was used as enrichment and concentrating probes for the enrichment of phosphopeptides from digested casein [26]. This approach was successfully applied to isolate and enrich 266 unique phosphopeptides with 340 phosphorylation sitesfrom rat liver mitochondria, offering detection limit of 30 amol for digest of β-casein.…”

Section: Maldi-msmentioning

confidence: 99%

Analytical Applications of Nanoparticles in Maldi-MS for Bioanalysis

Kailasa

D'souza

2015

Bioanalysis

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Recently, the rapid development of nanotechnology has enabled the analytical community to integrate processes with MALDI-MS for the analysis of various biomolecules. This article presents the recent progress on nanomaterials as extracting probes in single-drop microextraction, in liquid-liquid microextraction and as affinity probes for the enrichment of trace level biomolecules prior to their identification by MALDI-MS.

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“…For example, TiO 2 and iron oxide were coated onto a monolithic capillary column to enrich phosphopeptides from casein or human serum . Additionally, mesoporous TiO 2 and ZrO 2 aerogels were recently designed for selective enrichment of phosphopeptides in rat liver mitochondrion , where the binding capacity of mesoporous TiO 2 aerogel was shown to be six times higher than that of conventional TiO 2 microparticles for a single phosphopeptide standard. A total of 216 phosphoprotein groups were identified by the mesoporous TiO 2 aerogel from rat liver mitochondrion compared to less than 150 phosphoprotein groups identified by TiO 2 nanoparticles or microparticles .…”

Section: Advances In Enrichment Techniquesmentioning

confidence: 99%

Recent advances in enrichment and separation strategies for mass spectrometry‐based phosphoproteomics

Yang

Zhong

2014

Electrophoresis

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Due to the significance of protein phosphorylation in various biological processes and signaling events, new analytical techniques for enhanced phosphoproteomics have been rapidly introduced in recent years. The combinatorial use of the phospho-specific enrichment techniques and prefractionation methods prior to MS analysis enables comprehensive profiling of the phosphoproteome and facilitates deciphering the critical roles that phosphorylation plays in signaling pathways in various biological systems. This review places special emphasis on the recent five-year (2009–2013) advances for enrichment and separation techniques that have been utilized for phosphopeptides prior to MS analysis.

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Mesoporous TiO2 aerogel for selective enrichment of phosphopeptides in rat liver mitochondria

Cited by 33 publications

References 41 publications

The Potential Liver, Brain, and Embryo Toxicity of Titanium Dioxide Nanoparticles on Mice

The Potential Liver, Brain, and Embryo Toxicity of Titanium Dioxide Nanoparticles on Mice

Analytical Applications of Nanoparticles in Maldi-MS for Bioanalysis

Recent advances in enrichment and separation strategies for mass spectrometry‐based phosphoproteomics

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