Messenger RNA expression and immunolocalization of psoriasin in the goat mammary gland and its milk concentration after an intramammary infusion of lipopolysaccharide

Zhang, Gong-Wei; Lai, Song; Yoshimura, Y.; Isobe, Naoki

doi:10.1016/j.tvjl.2014.06.013

Cited by 41 publications

(65 citation statements)

References 26 publications

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“…Progesterone and S100A7 concentrations were measured as described previously (Isobe, Nakao, Yamashiro, & Shimada, ; Zhang, Lai, Yoshimura, & Isobe, ).…”

Section: Methodsmentioning

confidence: 99%

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Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Kuwahara

Yoshimura

Isobe

2017

Reprod Domestic Animals

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The objective of this study was to compare the dynamics of innate immune components after intramammary infusion of Staphylococcus aureus (SA) under conditions of high oestrogen and high progesterone in goats. In one group ("E-group"), controlled internal drug release (CIDR) devices were inserted intravaginally from days -11 to -4. Prostaglandin F2α was administered immediately after removal of the CIDR device at day -3, and then oestradiol benzoate (E) was injected intramuscularly once a day from days -2 to 3. Heat-inactivated SA was then administered via intramammary infusion to the left udder at day 0, whilst only saline was infused to the right udder as a control. In a second group ("P-group"), CIDR devices were inserted intravaginally from days -3 to 7 and SA was infused at day 0 in the same way as in the E-group. The milk yield and the concentration of innate immune components (somatic cell count (SCC), lactoferrin (LF), S100A7 and goat ß-defensin 1 (GBD-1)) in the milk were measured. Milk yield decreased drastically in both SA and control udders in the E-group, whereas the P-group exhibited increased milk yield in both SA and control udders. SCC increased after SA infusion in both E- and P-groups, although it was higher in the E-group than in the P-group. There was no significant change in LF concentration in the E-group, but a decrease was observed in the P-group. Concentrations of S100A and GBD-1 were significantly increased after SA infusion in the E-group but not in the P-group. These results suggest that E enhances the innate immune response induced by SA in the goat mammary gland. This effect may be due to the reduction in milk yield and upregulation of innate immune components.

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“…Progesterone and S100A7 concentrations were measured as described previously (Isobe, Nakao, Yamashiro, & Shimada, ; Zhang, Lai, Yoshimura, & Isobe, ).…”

Section: Methodsmentioning

confidence: 99%

“…After whole blood collection from the immunized rabbit, the GBD‐1 antibody was purified using a HiTrap Protein G High Performance affinity column (GH HealthCare, Uppsala, Sweden) according to the manufacturer's instructions. Concentration of GBD‐1 in milk was measured by competitive EIA, as described previously (Zhang et al., ,b).…”

Section: Methodsmentioning

confidence: 99%

Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Kuwahara

Yoshimura

Isobe

2017

Reprod Domestic Animals

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“…Immunohistochemical study using S100A7 antibody showed that epithelium in alveolus, teat skin and teat epithelium were positively stained (Zhang et al . ). At 24 h after the intramammary infusion of LPS, S100A7 concentration in milk was increased compared with that before infusion (Fig.…”

Section: S100a7 (S100 Calcium Binding Protein A7)mentioning

confidence: 97%

“…When different parts of goat mammary gland were taken and mRNA expression of S100A7 was investigated, its expression was high at teat skin and teat epithelium compared with those at alveolus and cistern (Zhang et al 2014a). In bovines, S100A7 mRNA was constitutively expressed in the streak canal (Tetens et al 2010).…”

Section: B-defensinmentioning

confidence: 99%

Control mechanisms for producing antimicrobial factors in ruminant mammary gland

Isobe

2017

Animal Science Journal

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Mastitis, a symptom of inflammation in mammary tissue by infection with various kinds of bacteria, causes huge economic losses in the milk industry. One of the popular methods for treatment of mastitis is antibiotics, although this prohibits milk shipping and sometimes causes resistant microbes. Therefore, a new strategy to treat mastitis without antibiotics is eagerly required around the world. Antimicrobial factors belong to innate immunity and can start their function extremely early after bacterial stimulation. These factors have antimicrobial activity for a broad spectrum of bacteria. Elucidation of causal mechanisms and functions of antimicrobial factors in the mammary gland is thought to result in suitable methods for prevention and treatment of mastitis. Therefore, this review introduces traits of some antimicrobial factors and the mechanisms for expressing, producing and secreting them in the mammary gland. For antimicrobial factors, lingual antimicrobial peptide (LAP), S100A7, cathelicidin and lactoferrin are controlled in different sites and different time courses, suggesting that antimicrobial factors play different roles for local defense against bacterial infection in the mammary gland. These findings will contribute to the development of prevention and treatment methods for mastitis.

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“…Concentrations of S100A7 and S100A8 were determined by enzyme immunoassay (EIA) as described previously (Isobe, Hosoda, & Yoshimura, 2009;Zhang, Lai, Yoshimura, & Isobe, 2014). Antibodies against synthetic S100A7 and S100A8 peptides consisting of 14 and 11 amino acids (CFEKQDKNKDRKID and CLEAHEIHKE), respectively, were raised in rabbits.…”

Section: Measurement Of S100a7 and S100a8mentioning

confidence: 99%

Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy

Elgawish

Ogata

Hidaka

et al. 2018

Reprod Domestic Animals

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This study was carried out to examine the changes in plasma concentrations of the Ca-binding antimicrobial proteins S100A7 and S100A8 during pregnancy in dairy cows. Holstein Friesian cows (n = 19) were inseminated with Holstein Friesian semen. Blood was collected at days 30, 60, 90, 120, 150, 180, 210, 240 and 270 after insemination. Plasma was used for measuring the concentrations of S100A7 and S100A8. Both S100A7 and S100A8 concentrations showed similar patterns during gestation; they increased during the midgestation, between days 90 and 180, and then declined before calving. The findings indicated that plasma concentrations of S100A7 and S100A8 did not change significantly during pregnancy in cows. Further studies are required to determine the roles of S100A7 and S100A8 in physiological function during pregnancy in dairy cows.

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Messenger RNA expression and immunolocalization of psoriasin in the goat mammary gland and its milk concentration after an intramammary infusion of lipopolysaccharide

Cited by 41 publications

References 26 publications

Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Control mechanisms for producing antimicrobial factors in ruminant mammary gland

Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy

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