MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

Mutlu, Beste; Chen, Huei Mei; Hall, David H.

doi:10.1101/429902

Cited by 2 publications

(1 citation statement)

References 92 publications

(130 reference statements)

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“…Wild-type embryonic development is characterized by rapid chromatin compaction, with cells achieving closed chromatin structures between the 100 to 200 cell stage (Mutlu et al 2018). While wild-type embryos show a moderate delay in this process at high temperature; DREAM complex mutants at high temperature show severe delays in the chromatin compaction process.…”

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confidence: 99%

Wnt Signaling Drives Ectopic Gene Expression and Larval Arrest in the Absence of theCaenorhabditis elegansDREAM Repressor Complex

Cherian¹,

Adams²,

Petrella

2020

G3 Genes|Genomes|Genetics

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Establishment and maintenance of proper gene expression is a requirement for normal growth and development. The DREAM complex in Caenorhabditis elegans functions as a transcriptional repressor of germline genes in somatic cells. At 26°, DREAM complex mutants show increased misexpression of germline genes in somatic cells and High Temperature Arrest (HTA) of worms at the first larval stage. To identify transcription factors required for the ectopic expression of germline genes in DREAM complex mutants, we conducted an RNA interference screen against 123 transcription factors capable of binding DREAM target promoter loci for suppression of the HTA phenotype in lin-54 mutants. We found that knock-down of 15 embryonically expressed transcription factors suppress the HTA phenotype in lin-54 mutants. Five of the transcription factors found in the initial screen have associations with Wnt signaling pathways. In a subsequent RNAi suppression screen of Wnt signaling factors we found that knock-down of the non-canonical Wnt/PCP pathway factors vang-1, prkl-1 and fmi-1 in a lin-54 mutant background resulted in strong suppression of the HTA phenotype. Animals mutant for both lin-54 and vang-1 showed almost complete suppression of the HTA phenotype, pgl-1 misexpression, and fertility defects associated with lin-54 single mutants at 26°. We propose a model whereby a set of embryonically expressed transcription factors, and the Wnt/PCP pathway, act opportunistically to activate DREAM complex target genes in somatic cells of DREAM complex mutants at 26°.

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confidence: 99%

Wnt Signaling Drives Ectopic Gene Expression and Larval Arrest in the Absence of theCaenorhabditis elegansDREAM Repressor Complex

Cherian¹,

Adams²,

Petrella

2020

G3 Genes|Genomes|Genetics

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Wnt signaling activates gene expression in the absence of theC. elegansDREAM repressor complex in somatic cells

Cherian

2019

Preprint

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Establishment and maintenance of proper gene expression is a requirement for normal growth and development. The DREAM complex in Caenorhabditis elegans functions as a transcriptional repressor of germline genes in somatic cells. At 26°C, DREAM complex mutants show temperature associated increase in misexpression of germline genes in somatic cells and High Temperature Arrest (HTA) of worms at the first larval stage. To identify transcription factors required for the ectopic expression of germline genes in DREAM complex mutants, we conducted an RNA interference screen against 123 transcription factors capable of binding DREAM target promoter loci for suppression of the HTA phenotype in lin-54 mutants. We found 15 embryonically expressed transcription factors that suppress the HTA phenotype in lin-54 mutants. Five of the transcription factors found in the initial screen interact with the Wnt signaling pathways.In a subsequent RNAi suppression screen of Wnt signaling factors we found that knockdown of the non-canonical Wnt/PCP pathway factors vang-1, prkl-1 and fmi-1 in lin-54 mutant background resulted in strong suppression of the HTA phenotype. Animals mutant for both lin-54 and vang-1 showed almost complete suppression of the HTA phenotype, pgl-1 misexpression, and fertility defects associated with lin-54 single mutants at 26°C. We propose a model whereby a set of embryonically expressed transcription factors, and the Wnt/PCP pathway, act opportunistically to activate DREAM complex target genes in somatic cells of DREAM complex mutants at 26°C. 4

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MET-2, a SETDB1 family methyltransferase, coordinates embryo events through distinct histone H3 methylation states

Cited by 2 publications

References 92 publications

Wnt Signaling Drives Ectopic Gene Expression and Larval Arrest in the Absence of theCaenorhabditis elegansDREAM Repressor Complex

Wnt Signaling Drives Ectopic Gene Expression and Larval Arrest in the Absence of theCaenorhabditis elegansDREAM Repressor Complex

Wnt signaling activates gene expression in the absence of theC. elegansDREAM repressor complex in somatic cells

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