ObjectiveThis research aimed to study the knowledge, attitudes, and practices (KAP) toward physical activity (PA) of healthcare students at King Khalid University in Abha, Saudi Arabia.MethodsA prospective web-based cross-sectional study was carried out among healthcare students of King Khalid University from March to May 2024. The questionnaire contained questions on sociodemographic details, five questions about knowledge, eight questions about attitudes, and seven practice-related questions. A multivariable logistic regression analysis was used.ResultsOut of the 383 participants, 175 (45.7%) were men and 208 (54.3%) were women. Most of the students, 292 (76.2%), had a body mass index (BMI) of 18.5–24.9. Among the 383, 264 (68.9%) showed good knowledge, 288 (75.2%) had positive attitudes, and 242 (63.2%) had satisfactory PA practice. Most of them, 310 (80.9%), were aware that “regular exercise helps promote strong bones and muscles.” Three hundred and fifty-two (91.9%) students believed that individuals who frequently engaged in PA had longer lifespans. More than half of the students (n = 239, 62.4%) engaged in the recommended level of PA. Of the students, 179 (46.8%) agreed that they planned to begin increasing their physical activity during the next 6 months. One hundred and fifty-six (40.7%) stated that they did sports with a high level of intensity for 20 min, three to five times a week, and 175 (45.7%) stated that they did moderate-intensity sports for 30 min, more than five times a week. None of the independent variables had a significant effect on good knowledge levels (p > 0.05). The regression results show being male OR = 0.473 (CI 0.285–0.783 p = 0.004), and being a first-or third-year student OR = 0.329 (CI 0.130–0.832 p = 0.013), OR = 0.276 (CI 0.100–0.762 p = 0.019) has a significant effect on positive attitude levels, while being male OR = 1.945 (CI 1.207–3.135 p = 0.006) and having a BMI between 18.5 and 24.9 OR = 10.101 (CI 2.104–48.49 p = 0.004) has a significant effect on satisfactory practice levels.ConclusionThe majority of participants in our study showed good knowledge and positive attitudes toward PA. A lower number of participants, though still the majority, had satisfactory levels of PA. Health education and health promotion initiatives should maintain and enhance knowledge of the positive effects of PA on health.