Meta-topolin induced morphometric and structurally stable bulblets in Malabar River Lily (Amaryllidaceae)

Manokari, M.; Priyadharshini, S.; Cokulraj, M.; Dey, Abhijit; Shekhawat, Mahipal S.

doi:10.1007/s11240-021-02195-z

Cited by 8 publications

(3 citation statements)

References 44 publications

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“…In the present study, mT was found more effective than BAP and Kn for multiple shoot bud induction in Dutch Iris. Similar effect of mT on in vitro shoot/bulblet formation from the basal plate of the twin scale explants was reported in a bulbous crop named Crinum malabaricum (Manokari et al, 2022). Several studies in various plant species have proved superiority of mT for in vitro shoot organogenesis and proliferation (Wojtania, 2010;Aremu et al, 2012;Gentile et al, 2014;Ahmad et al 2019).…”

Section: Assessment Of Genetic Delitysupporting

confidence: 56%

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Verma

Kumar

Priti

et al. 2022

Plant Cell Tiss Organ Cult

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Dutch Iris is a commercially important bulbous ornamental crop. Its high demand in global oriculture market necessitates the production of high-quality planting material. In the present investigation, the signi cance of meta-topolin (mT), a novel aromatic cytokinin, was studied on in vitro morphogenesis of Iris × hollandica Tub. cv. Professor Blaauw (Dutch Iris). Cytokinin mT (1.0 mg L − 1 ) as a better substitute to BAP (6-Benzylaminopurine) and Kn (Kinetin), has resulted in maximum shoot induction response (86.11 per cent) and increased emergence of micro shoots (4.95 shoots/explant with average shoot length of 5.65 cm) from twin scale explants. Photoperiod also affected the shoot induction, wherein initial 1-week (w) dark incubation signi cantly increased shoot induction response (94.44 per cent). Both meta-topolin and initial 1w dark incubation resulted in improved quality of regenerated shoots by reducing the incidence of necrosis and preventing vitri cation. The collective consequence of cytokininauxin (1.0 mg L − 1 mT + 0.25 mg L − 1 NAA) showed considerably higher number of shoots (17.53) with mean shoot length (6.90 cm) and maximum number of bulbets (2.74). Positive effect of increased sucrose concentration (90 g L 1 ) and paclobutrazol (5 mg L 1 ) on in vitro bulblet formation and bulblet size was also observed. The superiority of mT over BAP was also found during in vitro rhizogenesis. Shoots derived from mT medium were healthy and long enough, thus showed better rooting response (63.3 %) on ½ MS medium + 0.5 mg L − 1 IAA after 4 weeks. The mT raised plantlets were acclimatized in a greenhouse and showed 89.6 % survival under ex vitro conditions The clonal delity of the in vitro regenerated plants was evaluated using Start Codon Targeted (SCoT) markers. Out of 36 primers, 13 primers showed clearly scorable monomorphic bands, thus displaying genetic uniformity among in vitro regenerated plantlets. This meta-topolin mediated protocol can be routinely used for the rapid large scale production of this valuable oriculture crop.

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Section: Assessment Of Genetic Delitysupporting

confidence: 56%

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Verma

Kumar

Priti

et al. 2022

Plant Cell Tiss Organ Cult

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“…However, in recent studies, m T has been applied very frequently as an alternative to conventional cytokinins for the multiplications of shoots: Salvia sclarea 40 , Allamanda cathartica 29 , Oxystelma esculentum 31 , Rheum sp. ‘Malinowy’ 41 , Crinum malabaricum 30 , Crinum brachynema 42 and Caralluma umbellate 44 . Moreover, research on Crinum brachynema 42 and Aloe polyphylla 45 , similar to the presented results, showed that the 5 µM of m T is more favourable for the multiplication of shoots than higher concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…‘Malinowy’ 41 , Crinum malabaricum 30 , Crinum brachynema 42 and Caralluma umbellate 44 . Moreover, research on Crinum brachynema 42 and Aloe polyphylla 45 , similar to the presented results, showed that the 5 µM of m T is more favourable for the multiplication of shoots than higher concentrations. In our experiment, the addition of kinetin to the liquid medium was the least effective for stevia axillary shoot induction.…”

Section: Discussionmentioning

confidence: 99%

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Ptak,

Szewczyk,

Simlat

et al. 2023

Sci Rep

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Stevia rebaudiana Bertoni possesses various medicinal and food industrial applications. This study is the first to explore the effect of the cytokinins meta-Topolin (mT; 6-(3-hydroxybenzylamino) purine), zeatin, kinetin, and BAP (6-benzylaminopurine) at concentrations of 0 (control), 5, 10, and 15 µM on shoot multiplication, as well as stevioside, rebaudioside A, phenolic acid, and flavonoid content in bioreactor cultures. The highest number of shoots (23.4 per explant) was obtained in the medium containing 5 μM of mT. However, 15 μM of mT was superior for fresh biomass production and dry biomass accumulation. Reversed-phase (RP)-HPLC analysis showed a beneficial effect of 5 μM mT on stevioside (11.43 mg/g dry weight [DW]) and rebaudioside A (10.74 mg/g DW) biosynthesis. In all conditions, the ratio of rebaudioside A/stevioside ranged from 0.75 to 1.12. The phenolic acids chlorogenic, neochlorogenic, isochlorogenic A, and rosmarinic were confirmed in the stevia extracts, as were the flavonoids isoquercetin, and quercitrin. The highest accumulations of chlorogenic and neochlorogenic acids and flavonoids were observed in shoot tissues derived from 5 µM mT, whereas 5 µM of BAP stimulated biosynthesis of chlorogenic, isochlorogenic A, and rosmarinic acids. This is the first report on the use of mT-cytokinin showing high potential in stevia cultures.

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Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina

Dey

Roy²,

Mohture³

et al. 2022

Appl Microbiol Biotechnol

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Meta-topolin induced morphometric and structurally stable bulblets in Malabar River Lily (Amaryllidaceae)

Cited by 8 publications

References 44 publications

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina

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