Metabacillus bambusae sp. nov., isolated from bamboo grove soil

Abstract

A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization. BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium. BG109T showed growth at 10–40 °C (optimum, 37 °C), at pH 4–10 (optimum, 8), and in the presence of 0–7 % NaCl concentration (optimum, 0–1 %). The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso–diaminopimelic acid as the diagnostic diamino acid. The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present. The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0. The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus . BG109T shared highest 16S rRNA gene sequence similarities with ‘Metabacillus elymi’ KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction. The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus . Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus , for which the name Metabacillus bambusae sp. nov. is proposed. The type strain is BG109T (=KCTC 43190T=JCM 34515T).

AppIndels.com server: a web-based tool for the identification of known taxon-specific conserved signature indels in genome sequences. Validation of its usefulness by predicting the taxonomic affiliation of >700 unclassified strains of Bacillus species

“…Due to their group (taxon)-specificity and predictive ability to be found in other members of the indicated group, the identified CSIs have provided important means for the demarcation of prokaryotic taxa of different ranks in molecular terms, thus aiding in the development of more robust classification schemes for prokaryotic organisms [38, 39, 47, 48, 50, 52, 53, 58]. The CSIs have now been identified for numerous prokaryotic taxa [38, 39, 47, 48, 50, 52, 53, 58, 63, 93, 94] and several investigators have used them for the classification of newly described species into specific taxa [54–56, 62, 67–74]. However, the lack of a convenient method for determining the presence or absence of known CSIs in genome sequences has limited the use of these molecular markers for taxonomic and diagnostic studies.…”

“…In the present work, genome sequences were initially analysed for 394 uncharacterized Bacillus species, whose assembly levels were indicated as chromosome (12), complete (72) and contig (312). Subsequently, genomes for 327 additional uncharacterized Bacillus species with scaffold-level assembly were also analysed.…”

“…In the new classification scheme for Bacillus species, all newly described genera were reliably distinguished from each other (and other prokaryotic taxa) based on multiple identified CSIs which were specific for each of these genera [52,58]. Because of the predictive ability of the CSIs to be present in other members of these genera, the described CSIs have also been used by other investigators to assign newly described Bacillaceae species, as well as other species, into different genera [54][55][56][67][68][69][70][71][72][73][74]. Although the CSIs provide a very useful means for the circumscription of taxa of different ranks (specifically the genus-level clades), and for the assignment of new species into these genera based on the shared presence of CSIs, there is no convenient method available for determining the presence or absence of known taxon-specific CSIs in genome sequences.…”

Metabacillus arenae sp. nov., isolated from seashore sand

“…Currently this genus includes 19 validly described species according to the List of Prokaryotic names with Standing in Nomenclature (https://lpsn.dsmz.de/genus/metabacillus) [4]. Bacteria belonging to this genus have been isolated from various environments such as soil [5,6], hypersaline lake [7,8] and marine regions [2,[9][10][11][12]. During the study of the microbial diversity of Zhaoshu Island, an aerobic, Gram-stain-positive, rod-shaped bacterium, designated as strain IB182487 T , was isolated.…”