Metabolic and cellular alterations induced by diesel oil in Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta)

Ramlov, Fernanda; Carvalho, Tiago José Griebeler; Schmidt, Éder C.; Martins, Carla Braga; Kreusch, Marianne; Rodrigues, Eliseu; Bauer, Cláudia Marlene; Bouzon, Zenilda L.; Horta, Paulo Antunes; Maraschin, Marcelo

doi:10.1007/s10811-013-0209-y

Cited by 5 publications

(10 citation statements)

References 31 publications

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“…This species is widely distributed in southern Brazil near petroleum terminals, and therefore exposed to possible petroleum spills and its derivatives. Previous study with diesel oil, another petroleum derivative, showed a toxic effect and metabolic changes in H. musciformis seaweed in in vitro essays (Ramlov et al 2014). Diesel oil composition is variable, according to source of crude oil and process of refining.…”

Section: Introductionmentioning

confidence: 99%

“…Measurement of parameters related to metabolic rates maintenance, such as photosynthesis and antioxidant defenses, are more accurate for diagnosing organism's damage, and can be used to predict damage extent for populations or communities. In recent years, characterization and measurement of chlorophylls, carotenoids (both total or profile content), phenolic compounds and even polysaccharides have been extremely relevant to understanding acclimation capacity and tolerance that allows benthic macroalgae to survive environmental changes resulting from chemical pollution, as observed in an oil spill context (Ramlov et al 2014;Pilatti et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Gasoline exposure (Pilatti et al 2016), as well as ultraviolet radiation (Polo et al 2014), heavy metals (Santos et al 2015;Schiavon et al 2017) and diesel oil (Ramlov et al 2014) may promote significant changes in photosynthetic parameters, in low molecular-weight compounds such as carotenoids an accessory pigments, and in cell wall constituents like phenolic compounds. In addition, analysis of morphological changes in response to abiotic stress provides another way to confirm results from biochemical studies as well as those provided by microscopy techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Studies evaluating seaweed xenobiotic effects observed development of several strategies in order to tolerate diesel oil and gasoline presence. According to Ramlov et al (2014), longer exposure time (24 h) and concentration of diesel oil (1 %) decreased pigments concentration (chlorophyll and carotenoids), phenolic compounds, and starch grains content in Hypnea musciformis as a defense strategy against diesel oil penetration. However, Ulva lactuca increased soluble sugars and starch contents suggesting that U. lactuca is able to metabolize gasoline hydrocarbons and use them as an energy source.…”

Section: Introductionmentioning

confidence: 99%

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Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

et al. 2019

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Presence of toxic compounds in marine coastal waters has increased exponentially since Industrial Revolution. In this way, we aimed to evaluate biochemical and physiological changes occurring within Hypnea musciformis after short-term exposure to gasoline. Hypnea musciformis was cultivated without gasoline and then exposed to various concentrations of it (0.001 %-1.0 %, v/v) for periods of 30 min, 1 h, 12 h and 24 h. A Pricncipal Compound Analysis of UV-vis spectral window (200-700 nm) was able to discriminate gasoline-exposed samples according to both exposure time and gasoline concentration. Changes in carotenoid profile composition were observed. Decreased carotenoid content was associated to gasoline exposure time, being lutein and trans-β-carotene the major compounds found. Higher gasoline concentrations negatively interfered with phenolic compounds accumulation. In addition, increased gasoline concentrations corresponded to decreased intracellular starch grains content as well as increased its deposition on cell wall external surface. Data obtained allow us to conclude that gasoline can damage Hypnea musciformis physiology and cell morphology. This is important, considering Hypnea musciformis carotenoids and phenolics are potential biomarkers of environmental stress investigated, as well as its increased cell wall thickness to avoid gasoline diffusion.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

et al. 2019

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“…The gasoline sample presented a typical hydrocarbon composition, with paraffinic hydrocarbons that include the alkane series, the naphthene hydrocarbons comprising the cycloalkanes and the aromatic ones covering all compounds that contain one or more ring structures similar to benzene, aside from ethanol (35.1%, v/v) whose addition to gasoline is regulated by Brazilian National Agency of Petroleum (Pilatti et al, 2016). The diesel sample composition presented hydrocarbons compounds ranging from C6 to C30, with predominance of C8 to C18chain long hydrocarbons (Ramlov et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Metabolomics of Ulva lactuca Linnaeus (Chlorophyta) exposed to oil fuels: Fourier transform infrared spectroscopy and multivariate analysis as tools for metabolic fingerprint

Pilatti

Ramlov

Schmidt

et al. 2017

Marine Pollution Bulletin

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Fossil fuels, e.g. gasoline and diesel oil, account for substantial share of the pollution that affects marine ecosystems. Environmental metabolomics is an emerging field that may help unravel the effect of these xenobiotics on seaweeds and provide methodologies for biomonitoring coastal ecosystems. In the present study, FTIR and multivariate analysis were used to discriminate metabolic profiles of Ulva lactuca after in vitro exposure to diesel oil and gasoline, in combinations of concentrations (0.001%, 0.01%, 0.1%, and 1.0% - v/v) and times of exposure (30min, 1h, 12h, and 24h). PCA and HCA performed on entire mid-infrared spectral window were able to discriminate diesel oil-exposed thalli from the gasoline-exposed ones. HCA performed on spectral window related to the protein absorbance (1700-1500cm) enabled the best discrimination between gasoline-exposed samples regarding the time of exposure, and between diesel oil-exposed samples according to the concentration. The results indicate that the combination of FTIR with multivariate analysis is a simple and efficient methodology for metabolic profiling with potential use for biomonitoring strategies.

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In vitro exposure of Ulva lactuca Linnaeus (Chlorophyta) to gasoline – Biochemical and morphological alterations

et al. 2016

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Refined fuels have considerable share of pollution of marine ecosystems. Gasoline is one of the most consumed fuel worldwide, but its effects on marine benthic primary producers are poorly investigated. In this study, Ulva lactuca was chosen as a biological model due to its cosmopolitan nature and tolerance to high levels and wide range of xenobiotics and our goal was to evaluate the effects of gasoline on ultrastructure and metabolism of that seaweed. The experimental design consisted of in vitro exposure of U. lactuca to four concentrations of gasoline (0.001%, 0.01%, 0.1%, and 1.0%, v/v) over 30 min, 1 h, 12 h, and 24 h, followed by cytochemical, SEM, and biochemical analysis. Increase in the number of cytoplasmic granules, loss of cell turgor, cytoplasmic shrinkage, and alterations in the mucilage were some of the ultrastructural alterations observed in thalli exposed to gasoline. Decrease in carotenoid and polyphenol contents, as well as increase of soluble sugars and starch contents were associated with the time of exposure to the xenobiotic. In combination, the results revealed important morphological and biochemical alterations in the phenotype of U. lactuca upon acute exposure to gasoline. This seaweed contain certain metabolites assigned as candidates to biomarkers of the environmental stress investigated and it is thought to be a promise species for usage in coastal ecosystems perturbation monitoring system. In addition, the findings suggest that U. lactuca is able to metabolize gasoline hydrocarbons and use them as energy source, acting as bioremediator of marine waters contaminated by petroleum derivatives.

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Metabolic and cellular alterations induced by diesel oil in Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta)

Cited by 5 publications

References 31 publications

Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

Hypnea musciformis (Wulfen) J. V. Lamour. (Gigartinales, Rhodophyta) responses to gasoline short-term exposure: biochemical and cellular alterations

Metabolomics of Ulva lactuca Linnaeus (Chlorophyta) exposed to oil fuels: Fourier transform infrared spectroscopy and multivariate analysis as tools for metabolic fingerprint

In vitro exposure of Ulva lactuca Linnaeus (Chlorophyta) to gasoline – Biochemical and morphological alterations

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