Metabolic annotation, interactions and characterization of natural products of mango (Mangifera indica L.): 1H NMR based chemical metabolomics profiling

Angamuthu, Sudha; Ramaswamy, Chidambaram R.; Selvankumar, Thangaswamy; Sadhasivam, Deepa Rani; Nallaswamy, V. Deepak; Raghunandhakumar, Subramanian; Ganesan, Raja; Raju, Amutha

doi:10.1016/j.procbio.2021.05.024

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…Metabolomics has been extensively developed in herbal research identifying the authenticity of medicinal plants [ 9 ], determining the harvest time of raw medicinal materials [ 10 ], exploring the effects of soil characteristics on changes in the plant metabolome [ 11 ], screening processing methods [ 12 ] and other factors. Metabolomic approaches provide valuable resources for the identification of metabolite differences between mutant and wild-type plants, the identification of metabolite changes in original herbs, and the quality control of herbal medicines [ 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Does the Metabolome of Wild-like Dendrobium officinale of Different Origins Have Regional Differences?

Lan

Liu

et al. 2022

Molecules

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Dendrobium officinale, as a traditional Chinese medicine, has considerable commercial value and pharmacological activity. Environmental factors of different origins have a great influence on Dendrobium officinale metabolites, which affect its pharmacological activity. This study sought to identify the differential metabolites of wild-imitating cultivated D. officinale stems of different origins. Using the widely-targeted metabolomics approach, 442 metabolites were detected and characterized, including flavonoids, lipids, amino acids and derivatives, and alkaloids. We found that although the chemical constitution of D. officinale cultured in the three habitats was parallel, the contents were significantly different. Meanwhile, the KEGG pathway enrichment analysis revealed that the distinctive metabolites among the three groups were mainly involved in flavone and flavonol biosynthesis. To further explore the different contents of flavonoids, HPLC was performed on four main flavonoid contents, which can be used as one of the references to distinguish D. officinale from different growing origins. In conclusion, a comprehensive profile of the metabolic differences of D. officinale grown in different origins was provided, which contributed a scientific basis for further research on the quality evaluation of D. officinale.

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Section: Introductionmentioning

confidence: 99%

Does the Metabolome of Wild-like Dendrobium officinale of Different Origins Have Regional Differences?

Lan

Liu

et al. 2022

Molecules

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“…Finally, the spectra were split into 0.04 ppm buckets from the δ = 0.2-10 region and exported as a commaseparated value (CSV) file [44]. The chemometric analysis of the data was performed using MetaboAnalyst V.4.0 (https://www.metaboanalyst.ca, accessed on 24 March 2023), where HCA, PCA [45,46], and OPLS-DA were carried out, allowing the correlation of the signals of the 1 H-NMR profiles with the biological activity data [24]. For the identification of the active metabolites, the 1 H-NMR signals selected via OPLS-DA were used, and 13 C analysis, COSY, HSQC, HMBC and TOCSY were performed on the sample with the highest nematocidal activity [11].…”

Section: H-nmr Metabolic Profiling and Multivariate Analysismentioning

confidence: 99%

1H-NMR Metabolomic Study of the Mushroom Pleurotus djamor for the Identification of Nematocidal Compounds

Pineda-Alegría,

Peña-Rodríguez,

Cardoso-Taketa

et al. 2024

Pharmaceuticals

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Due to the increasing populations of anthelmintic-resistant gastrointestinal nematodes and as a consequence of the adverse effects of synthetic drugs, this study focuses on the search for secondary metabolites with nematocidal activity from the edible mushroom Pleurotus djamor using The proton nuclear magnetic resonance (1H-NMR) metabolomics. The highest activity was shown by the ethyl acetate fractions of mycelium (EC50 290.8 µg/mL) and basidiomes (EC50 282.7 µg/mL). Principal component analysis (PCA) and hierarchical data analysis (HCA) of the 1H-NMR metabolic profiles data showed that the ethanolic extracts, the ethyl acetate, butanol, and water fractions from mycelium have different metabolic profiles than those from basidiomes, while low polarity (hexane) fractions from both stages of fungal development show similar profiles. Orthogonal partial least squares discriminant analysis (OPLS-DA) allowed the identification of signals in the 1H-NMR metabolic profile associated with nematocidal activity. The signals yielded via OPLS-DA and bidimensional NMR analysis allowed the identification of uracil as a component in the ethyl acetate fraction from basidiomes, with an EC50 of 237.7 µg/mL. The results obtained showed that chemometric analyses of the 1H-NMR metabolic profiles represent a viable strategy for the identification of bioactive compounds from samples with complex chemical profiles.

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“…However, multidrug resistance leads to an increased period of hospitalization and increased mortality [ 7 ]. A matter of great concern is the emergence of drug-resistant Shigella strains, which has led to treatment impasse, and treatment of diarrheal illness has become challenging [ 1 , 8 ]. Therefore, there is an urgent need to develop novel therapeutic agents that can arrest the growth of drug-resistant foodborne pathogens, such as Shigella .…”

Section: Introductionmentioning

confidence: 99%

Biogenic Preparation and Characterization of Silver Nanoparticles from Seed Kernel of Mangifera indica and Their Antibacterial Potential against Shigella spp.

Angamuthu¹,

Selvankumar²,

Raju

et al. 2023

Molecules

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Shigellosis is a serious foodborne diarrheal disease caused by the Shigella species. It is a critical global health issue. In developing countries, shigellosis causes most of the mortality in children below 5 years of age. Globally, around 165 million cases of diarrhea caused by Shigella are reported, which accounts for almost 1 million deaths, in which the majority are recorded in Third World nations. In this study, silver nanoparticles were synthesized using Mangifera indica kernel (MK-AgNPs) seed extracts. The biosynthesized M. indica silver nanoparticles (MK-AgNPs) were characterized using an array of spectroscopic and microscopic tools, such as UV–Vis, scanning electron microscopy, particle size analyzer, Fourier transform infrared spectroscopy, and X-ray diffractometer. The nanoparticles were spherical in shape and the average size was found to be 42.7 nm. The MK-AgNPs exhibited remarkable antibacterial activity against antibiotic-resistant clinical Shigella sp. The minimum inhibitory concentration (MIC) value of the MK-AgNPs was found to be 20 μg/mL against the multi-drug-resistant strain Shigella flexneri. The results clearly demonstrate that MK-AgNPs prepared using M. indica kernel seed extract exhibited significant bactericidal action against pathogenic Shigella species. The biosynthesized nanoparticles from mango kernel could possibly prove therapeutically useful and effective in combating the threat of shigellosis after careful investigation of its toxicity and in vivo efficacy.

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Metabolic annotation, interactions and characterization of natural products of mango (Mangifera indica L.): 1H NMR based chemical metabolomics profiling

Cited by 8 publications

References 48 publications

Does the Metabolome of Wild-like Dendrobium officinale of Different Origins Have Regional Differences?

Does the Metabolome of Wild-like Dendrobium officinale of Different Origins Have Regional Differences?

1H-NMR Metabolomic Study of the Mushroom Pleurotus djamor for the Identification of Nematocidal Compounds

Biogenic Preparation and Characterization of Silver Nanoparticles from Seed Kernel of Mangifera indica and Their Antibacterial Potential against Shigella spp.

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