Metabolic Changes Induced in Human Spermatozoa by Subagglutinating Concentrations of Specific Antibodies

Abstract

The effects on sperm metabolism of subagglutinating concentrations of infertile female sera, containing antisperm antibody, were studied. Sera were selected by their differential agglutinating or immobilizing properties and by high titers of activity. Only the immobilizing antibodies were able to decrease sperm viability. Basal and succinate depending oxygen uptake, thus as progressive motility was decreased by sera producing mixed agglutination and more drastically by that producing immobilization independent of the presence of complement. The sera with head-to-head agglutination properties induced an increase in the above mentioned physiologic parameters. Tip of tail-to-tip of tail agglutinating sera having no effect on oxygen uptake were able to induce a significant increase in progressive motility. Conversion of IT-Glucose to 14C0, was decreased by sera that induced tip of tail-to-tip of tail agglutination and complementindependent immobilization. The sera which induced head-to-head agglutination and immobilization on the presence of complement, were without effect. None of the tested sera showed any activity over the conversion of 'T-Glucose to %Lactate.

Immunochemical Assays and Nucleic-Acid Detection Techniques for Clinical Diagnosis of Prostate Cancer

“…Acid phosphatases are a group of tissue isoenzymes that can hydrolyse organic monophosphate esters and are found in red blood cells, kidney, lung, liver, platelets and osteoclasts. In healthy patients, the concentration of serum prostatic acid phosphatase (PAP) typically lies is in the range 8 - 16 μg/l 15 but can be over a hundredfold more abundant in the prostate than in other tissues 14 . Evaluation of PAP activity particularly in human serum became clinically relevant after it was discovered that its activity is not only high as a consequence of PCa 16 , 17 , but also increases with disease progression 18 .…”

“… 14 . In contrast to RIA, another immunoassay for PAP known as counter-immunoelectrophoresis (CIEP) 15 , 26 was favoured for its simplicity, higher sensitivity (as little as 1.0 ng of PAP could be detected in serum) and the short period (2 hrs) required to complete a test.…”