Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway

Cen, Xuecong; Liu, Yanjuan; Zhu, Fanghuan; Liu, Dehua; Chen, Zhe

doi:10.1016/j.ymben.2022.10.012

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…Organisms do not possess natural biosynthetic pathways to produce 1,5-PDO. Previous studies have reported three lysine-derived artificial pathways for 1,5-PDO bioproduction, which were successfully established in E. coli [ 5 – 7 ]. A recent study systematically compared eight potential pathways for converting lysine into 1,5-PDO and reported that a cadaverine-based pathway has an advantage in producing 1,5-PDO due to its high theoretical yield [ 7 ].…”

Section: Resultsmentioning

confidence: 99%

“…1,5-PDO as an important chemical intermediate plays an indispensable role in industries such as polyester and coatings, which has a broad range of downstream applications and promising market prospects. However, only three feasible pathways for producing 1,5-PDO in E. coli have been reported [ 5 – 7 ], so it is necessary to develop and expand new pathways for biosynthesis of 1,5-PDO. In this study, we proposed a novel pathway for synthesizing 1,5-PDO, which was composed of lysine decarboxylase, aminotransferase, and alcohol dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

“…Based on previous research, Cen et al reported an efficient cadaverine-based pathway to produce 1,5-PDO through seven steps. lysine decarboxylase (CadA), cadaverine aminotransferase (PatA), glutaraldehyde dehydrogenase (PatD), aminotransferase, carboxylate reductase and alcohol dehydrogenase [ 7 ]. The further development of artificial 1,5-PDO biosynthetic pathway is benefit for expanding new methods for its bioproduction.…”

Section: Introductionmentioning

confidence: 99%

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An integrated cofactor and co-substrate recycling pathway for the biosynthesis of 1,5-pentanediol

Hua,

Liang,

Zhou

et al. 2024

Microb Cell Fact

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Background 1,5-pentanediol (1,5-PDO) is a linear diol with an odd number of methylene groups, which is an important raw material for polyurethane production. In recent years, the chemical methods have been predominantly employed for synthesizing 1,5-PDO. However, with the increasing emphasis on environmentally friendly production, it has been a growing interest in the biosynthesis of 1,5-PDO. Due to the limited availability of only three reported feasible biosynthesis pathways, we developed a new biosynthetic pathway to form a cell factory in Escherichia coli to produce 1,5-PDO. Results In this study, we reported an artificial pathway for the synthesis of 1,5-PDO from lysine with an integrated cofactor and co-substrate recycling and also evaluated its feasibility in E.coli. To get through the pathway, we first screened aminotransferases originated from different organisms to identify the enzyme that could successfully transfer two amines from cadaverine, and thus GabT from E. coli was characterized. It was then cascaded with lysine decarboxylase and alcohol dehydrogenase from E. coli to achieve the whole-cell production of 1,5-PDO from lysine. To improve the whole-cell activity for 1,5-PDO production, we employed a protein scaffold of EutM for GabT assembly and glutamate dehydrogenase was also validated for the recycling of NADPH and α-ketoglutaric acid (α-KG). After optimizing the cultivation and bioconversion conditions, the titer of 1,5-PDO reached 4.03 mM. Conclusion We established a novel pathway for 1,5-PDO production through two consecutive transamination reaction from cadaverine, and also integrated cofactor and co-substrate recycling system, which provided an alternative option for the biosynthesis of 1,5-PDO.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An integrated cofactor and co-substrate recycling pathway for the biosynthesis of 1,5-pentanediol

Hua,

Liang,

Zhou

et al. 2024

Microb Cell Fact

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“…The strain consisting of these changes resulted in an overall titer of 9.25 g/L of 1,5-PDO, which is significantly higher than the previous study but is still considered low on the industry scale. Furthermore, a high accumulation of byproducts still remained as well, therefore, this engineered pathway needs to be further optimized and tested [20].…”

Section: 5-pentanediol (15-pdo)mentioning

confidence: 99%

“…The red pathway corresponds to the reference[60]. The green pathway coincides with the reference[20]. Light olive pathway correlates to reference[61].…”

mentioning

confidence: 92%

Metabolic Engineering of Microorganisms Towards the Biomanufacturing of Non-Natural C5 and C6 Chemicals

Tseng,

Nguyen,

Lin

2023

Synthetic Biology and Engineering

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Five-carbon (C5) and six-carbon (C6) chemicals are essential components in the manufacturing of a variety of pharmaceuticals, fuels, polymers, and other materials. However, the predominant reliance on chemical synthesis methods and unsustainable feedstock sources has placed significant strain on Earth's finite fossil resources and the environment. To address this challenge and promote sustainability, significant efforts have been undertaken to re-program microorganisms through metabolic engineering and synthetic biology approaches allowing for biobased manufacturing of these compounds. This review provides a comprehensive overview of the advancements in microbial production of commercially significant non-natural C5 chemicals, including 1-pentanol, 1,5-pentanediol, cadaverine, δ-valerolactam, glutaric acid, glutaconic acid, and 5-hydroxyvaleric acid, as well as C6 chemicals, including cis,cis-muconic acid, adipic acid, 1,6-hexamethylenediamine, 6-aminocaproic acid, β-methyl-δ-valerolactone, 1-hexanol, ε-caprolactone, 6-hydroxyhexanoic acid, and 1,6-hexanediol.

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Design an Energy‐Conserving Pathway for Efficient Biosynthesis of 1,5‐Pentanediol and 5‐Amino‐1‐Pentanol

Ma,

Xie,

Zhang

et al. 2024

Biotech & Bioengineering

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ABSTRACT1,5‐Pentanediol (1,5‐PDO) is an important five‐carbon alcohol, widely used in polymer and pharmaceutical industries. Considering the substantial energy (ATP and NADPH) requirements of previous pathways, an energy‐conserving artificial pathway with a higher theoretical yield (0.75 mol/mol glucose) was designed and constructed in this study. In this pathway, lysine is converted into 1,5‐PDO by decarboxylation, two transamination, and two reduction reactions. For the purpose of full pathway construction, 5‐aminopetanal reductase and 5‐amino‐1‐pentanol (5‐APO) transaminase were identified and characterized. By implementing strategies such as modular optimization of gene expression, enhancing lysine biosynthesis and increasing NADPH supply, the engineered strains were able to produce 1502.8 mg/L 5‐APO and 726.2 mg/L 1,5‐PDO in shake flasks and 11.7 g/L 1,5‐PDO in a 3 L bioreactor. This work provides a new and promising pathway for the efficient production of 5‐APO and 1,5‐PDO.

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Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway

Cited by 11 publications

References 44 publications

An integrated cofactor and co-substrate recycling pathway for the biosynthesis of 1,5-pentanediol

An integrated cofactor and co-substrate recycling pathway for the biosynthesis of 1,5-pentanediol

Metabolic Engineering of Microorganisms Towards the Biomanufacturing of Non-Natural C5 and C6 Chemicals

Design an Energy‐Conserving Pathway for Efficient Biosynthesis of 1,5‐Pentanediol and 5‐Amino‐1‐Pentanol

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