Metabolic‐Glycoengineering‐Enabled Molecularly Specific Acoustic Tweezing Cytometry for Targeted Mechanical Stimulation of Cell Surface Sialoglycans
Weiping Li,
Jiatong Guo,
Eric C. Hobson
et al.
Abstract:This study developed a new type of dibenzocyclooctyne (DBCO)‐functionalized microbubbles (MBs) and validated their attachment to azide‐labelled sialoglycans on human pluripotent stem cells (hPSCs) generated via metabolic glycoengineering (MGE). This enabled application of mechanical forces to sialoglycans on hPSCs via molecularly specific acoustic tweezing cytometry (mATC), i.e., displacing sialoglycan‐anchored MBs using ultrasound (US). It was shown that subjected to forces of US pulses, sialoglycan‐anchored … Show more
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