Metabolic profiling for studying chemotype variations in Withania somnifera (L.) Dunal fruits using GC–MS and NMR spectroscopy

Bhatia, Ashok Kumar; Bharti, Santosh Kumar; Tewari, Shri Krishna; Sidhu, O. P.; Roy, Raja

doi:10.1016/j.phytochem.2013.03.013

Cited by 74 publications

(32 citation statements)

References 43 publications

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“…β-Elemene is one of the essential oils constituent that is poorly represented in the most accessions and its occurrence is limited in two accessions (accession number 27 and 28) in which its content is higher than 10%. In agreement with these results, Grayer et al [21] reported that the essential oil compositions appeared to be very different in the various accessions of O. basilicum, and some compounds were specific to a single variety; similar results were also obtained by Bhatia et al [29] . Variation in the metabolites concentration among different chemotypes of Withania somnifera fruits suggested that specific chemotypes can be used to obtain substantial amounts of bioactive ingredients for use as potential pharmacological and nutraceuticals agents.…”

Section: Resultssupporting

confidence: 71%

“…Variation in the metabolites concentration among different chemotypes of Withania somnifera fruits suggested that specific chemotypes can be used to obtain substantial amounts of bioactive ingredients for use as potential pharmacological and nutraceuticals agents. [29] So, the concentration of active components is one of the major factors that affected the medicinal plants' quality, as well as the minor compounds are important in respect of biological activity and they are critical for the activity of the oil and may have synergistic effects. [10,30] To characterize and verify the variations of the essential oils and identify the different possible chemotypes in the accessions of O. ciliatum, their compositions were analyzed by cluster analysis.…”

Section: Resultsmentioning

confidence: 99%

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Variability in essential oil content and composition of Ocimum ciliatum accessions from Iran: evidence for three chemotypes

Moghaddam

Farhadi

Ranjbar

2017

International Journal of Food Properties

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The chemical composition of essential oils isolated from aerial parts of 28 accessions of Ocimum ciliatum from Iran was investigated by gas chromatography and gas chromatography-mass spectrometry. The oil contents were found to be about 0.71-1.25% (w/w). In total, 48 compounds, accounting for 93.7-99.8% of the oils composition, were identified. The results from the composition suggested that the oils could be classified as aromatic/ oxygenated monoterpene-type. Among the aromatic compounds, the main components were methyl chavicol (11.6-89.6%) and citral (0.5-50.7%), which were the main oxygenated monoterpenes in all essential oils. The chemotypes values of the essential oils composition were discussed according to the results of cluster analysis. The 28 accessions were divided into 3 groups, each comprising a chemotype. The different characterized chemotypes were chemotype I (methyl chavicol), chemotype II (citral), and chemotype III (methyl chavicol/citral).

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Section: Resultssupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Variability in essential oil content and composition of Ocimum ciliatum accessions from Iran: evidence for three chemotypes

Moghaddam

Farhadi

Ranjbar

2017

International Journal of Food Properties

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“…GC-MS analysis was carried out for aqueous soluble content of infected and healthy poppy leaves and the green capsules (Bhatia et al, 2013) using Thermo Trace GC Ultra coupled with Thermo fisher DSQ II mass spectrometers. Chromatographic separations of metabolites were carried out on 30 m × 0.25 mm Thermo TR50 column (polysiloxane column coated with 50% methyl and 50% phenyl groups).…”

Section: Methodsmentioning

confidence: 99%

Ageratum enation virus Infection Induces Programmed Cell Death and Alters Metabolite Biosynthesis in Papaver somniferum

et al. 2017

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A previously unknown disease which causes severe vein thickening and inward leaf curl was observed in a number of opium poppy (Papaver somniferum L.) plants. The sequence analysis of full-length viral genome and associated betasatellite reveals the occurrence of Ageratum enation virus (AEV) and Ageratum leaf curl betasatellite (ALCB), respectively. Co-infiltration of cloned agroinfectious DNAs of AEV and ALCB induces the leaf curl and vein thickening symptoms as were observed naturally. Infectivity assay confirmed this complex as the cause of disease and also satisfied the Koch’s postulates. Comprehensive microscopic analysis of infiltrated plants reveals severe structural anomalies in leaf and stem tissues represented by unorganized cell architecture and vascular bundles. Moreover, the characteristic blebs and membranous vesicles formed due to the virus-induced disintegration of the plasma membrane and intracellular organelles were also present. An accelerated nuclear DNA fragmentation was observed by Comet assay and confirmed by TUNEL and Hoechst dye staining assays suggesting virus-induced programmed cell death. Virus-infection altered the biosynthesis of several important metabolites. The biosynthesis potential of morphine, thebaine, codeine, and papaverine alkaloids reduced significantly in infected plants except for noscapine whose biosynthesis was comparatively enhanced. The expression analysis of corresponding alkaloid pathway genes by real time-PCR corroborated well with the results of HPLC analysis for alkaloid perturbations. The changes in the metabolite and alkaloid contents affect the commercial value of the poppy plants.

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“…It was further observed that ratio of these two withanolides was a major discriminating feature of W. somnifera leaf samples from different origins (Namdeo et al, 2011). Likewise, NMR technique has also been employed for qualitative and quantitative analysis of metabolites in W. somnifera fruits in different chemotypes (Bhatia, Bharti, Tewari, Sidhu, & Roy, 2013) and during different stages of development . It was observed that the early stages of fruit development had relatively higher concentrations of withanolides, alanine, aspartate, choline, phosphocholine, sucrose, and caffeic acid, whereas higher accumulation of citrate and withanamides was observed during maturation phase.…”

Section: Biotechnological Interventions Inmentioning

confidence: 99%

Biotechnological interventions inWithania somnifera(L.) Dunal

Singh

Guleri

Singh

et al. 2015

Biotechnology and Genetic Engineering Reviews

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Withania somnifera is one of the most valued plants and is extensively used in Indian, Unani, and African systems of traditional medicine. It possess a wide array of therapeutic properties including anti-arthritic, anti-aging, anti-cancer, anti-inflammatory, immunoregulatory, chemoprotective, cardioprotective, and recovery from neurodegenerative disorders. With the growing realization of benefits and associated challenges in the improvement of W. somnifera, studies on exploration of genetic and chemotypic variations, identification and characterization of important genes, and understanding the secondary metabolites production and their modulation has gained significant momentum. In recent years, several in vitro and in vivo preclinical studies have facilitated the validation of therapeutic potential of the phytochemicals derived from W. somnifera and have provided necessary impetus for gaining deeper insight into the mechanistic aspects involved in the mode of action of these important pharmaceutically active constituents. The present review highlights some of the current developments and future prospects of biotechnological intervention in this important medicinal plant.

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Metabolic profiling for studying chemotype variations in Withania somnifera (L.) Dunal fruits using GC–MS and NMR spectroscopy

Cited by 74 publications

References 43 publications

Variability in essential oil content and composition of Ocimum ciliatum accessions from Iran: evidence for three chemotypes

Variability in essential oil content and composition of Ocimum ciliatum accessions from Iran: evidence for three chemotypes

Ageratum enation virus Infection Induces Programmed Cell Death and Alters Metabolite Biosynthesis in Papaver somniferum

Biotechnological interventions inWithania somnifera(L.) Dunal

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