Metabolism of sodium oestrone [35S]sulphate in the rat

Dolly, J. Oliver; Cg, Curtis; Ks, Dodgson; Fa, Rose

doi:10.1042/bj1230261

Cited by 14 publications

(11 citation statements)

References 27 publications

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“…radioactivity approx. 6mCi/mmol) as described by Dolly et al (1971). The potassium salts of p-acetylphenyl sulphate and p-nitrophenyl sulphate were synthesized by the method of Burkhardt & Lapworth (1926) as modified by Milsom et al (1972).…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

“…However, it is noteworthy that Hanahan & Everett (1950) have shown that injected sodium oestrone [35S]sulphate undergoes appreciable desulphation in the rat with the rapid excretion of inorganic [35S]sulphate in the urine. Moreover, the liver was found to be the principal organ of the rat where the desulphation of injected oestrone [35S]_ sulphate occurs (Dolly et al, 1971). Several investi-gators have reported that rat and human tissues have the capacity to desulphate oestrone sulphate (see Pulkkinen, 1961;Notation & Ungar, 1969;Pack & Brooks, 1970;Schwers & Rodesch, 1963;Sandberg & Jenkins, 1966), the responsible enzyme being localized in the microsomal fraction of the cell (Pulkkinen & Paunio, 1963;Zuckerman &Hagerman, 1969;French & Warren, 1966).…”

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confidence: 99%

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Studies of the oestrogen sulphatase and arylsulphatase C activities of rat liver

Dolly¹,

Dodgson²,

Rose³

1972

Biochemical Journal

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Detailed studies on the hydrolysis of p-acetylphenyl sulphate and oestrone sulphate by rat liver preparations strongly indicate that arylsulphatase C and oestrogen sulphatase are the same enzyme. Liver is the richest source of both enzymes, which have identical intracellular distributions, being localized mainly in the microsomal fraction. Low oestrogen sulphatase and arylsulphatase C activities were present in foetal liver and these increased at a similar rate after birth. The activities of the enzymes in an ethionine-induced hepatoma were similarly low. Results of heat inactivation, mixed-substrate and competitive-inhibition experiments employing liver microsomal fractions were also consistent with one enzyme being involved. Oestradiol-17beta 3-sulphate was also hydrolysed by microsomal preparations and activity towards both this substrate and oestrone sulphate was inhibited by oestrone and oestradiol-17beta. The physiological significance of this inhibition is discussed.

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Section: Materials and Methods Materialsmentioning

confidence: 99%

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confidence: 99%

Studies of the oestrogen sulphatase and arylsulphatase C activities of rat liver

Dolly¹,

Dodgson²,

Rose³

1972

Biochemical Journal

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“…After incubation of oestradiol with rat liver preparations hy¬ droxylation has also been shown to occur at C-2, C-6 and C-l6 (Hagopian 8c Levy 1958;Breuer 8c Knuppen 1960;Favu et al 1969;Brooks 8c Horn 1971;Worlmann et al 1972). Oestrone sulphate administered intraperitoneally or intravenously is rapidly hydrolysed and the sulphate radical is rapidly excreted in the urine (Dolly et al 1971). In animals with the bile duct catherized, only small amounts of radioactivity were detected in bile and this was mainly in the form of oestrone and oestradiol sulphates.…”

Section: G Metabolism Of Oestrogens In Ratsmentioning

confidence: 99%

Metabolism of Synthetic Steroids by Animals and Man

Fotherby

1974

Acta Endocrinologica

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The metabolism of naturally occurring oestrogens in humans is outlined and compared with that in other species and with the metabolism of synthetic oestrogens. There is a close similarity in humans between the metabolism of the naturally occurring oestrogens and that of ethynyl \x=req-\ oestradiol with the exception that the latter is less extensively metabolised than the former. With the exception of the baboon, the metabolism of oestrone and oestradiol appears to differ significantly in other species from that of the human. Our knowledge of the metabolism of oestrone and oestradiol and the synthetic oestrogens in the baboon is still meagre and much more information is required before one can ascertain to what extent metabolism in this species can serve as a model for that in humans.Little information is available concerning the metabolism of synthetic progestogens in species other than man and rabbits. There are many similarities between these two species both in the metabolism of progesterone and of synthetic progestogens related both to 19-nortestosterone and to 17\g=a\-hydroxyprogesterone. Insufficient information is available to know how far these similarities extend.Little is known about the in vitro metabolism of either the synthetic oestrogens or synthetic progestogens. Such information as is available does not suggest that in vitro metabolic studies, with their well-known limitations, are likely to be helpful. In vivo metabolism of oestrogensThe metabolism in humans of the naturally occurring oestrogens is briefly outlined to aid comparison with that in other species and also with the meta¬ bolism of synthetic oestrogens.A. Metabolism of oestrone and oestradiol in humans The major pathways for the metabolism of oestrogens are outlined in Fig. 1.Oestrone and oestradiol are interconvertible by 17/3-hydroxysteroid oxidoreductase, the dehydrogenation of oestradiol to oestrone being more rapid than the reduction of oestrone to oestradiol so that the equilibrium between oestrone and oestradiol favours oestrone. Although a 17a-hydroxysteroid oxidoreductase is present in man (Breuer 8c Schott 1966), 17a-oestradiol is not an important metabolite but it is important in some species. One of the main metabolic pathways for oestrone is 16a-hydroxylation leading to 16a-hydroxyoestrone and oestriol. Some of the oestriol produced is oxidized to 16-oxo-oestradiol which can undergo reduction to 16/?-hydroxyoestradiol (16-e/«oestriol). The second major pathway appears to be hydroxylation at C-2 with the formation of 2-hydroxyoestrone which is readily converted to 2-methoxyoestrone by an 0-methyl transferase. Demethylation of 2-methoxyoestrogens occurs to only a slight extent. It seems likely that oestrone sulphate rather than oestrone is the precursor for the hydroxylated metabolites found in urine (Fishman 8c Hellman 1973). Hydroxylation may also occur at C-6. Oestradiol -17 a Ha^/V^" HC 2-Hydroxyoestrone 16a-Hydroxyoestrone lóB-Hydroxyoestrone * OH O Me O HO OH -OH 2-Methoxyoestrone Oestriol Fig. 1.Metabolism of oestrog...

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“…The relative amount of radioactivity associated with each area was measured from the record of the scanner as described by Dodgson and Tudball (12). Total radioactivity in liquid samples was also determined by scintillation counting by the method of Dolly et al (13).…”

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Liberation of sulfate from sulfate esters by soils

Houghton¹,

Rose²

1976

Appl Environ Microbiol

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When incubated with acid, alkaline, and neutral soils, a variety of synthetic sulfate esters representing the various classes of these compounds was hydrolyzed by enzymes, probably of microbial origin. The appearance of sulfate in the soil water occurred immediately after introduction into the soils with some esters, whereas with others it occurred only after lag periods. Heat treatment destroyed the hydrolytic activity in the soils. The ester sulfate groups-present in humic acid extracted from the soil appeared to be resistant to hydrolysis by a variety of sulfohydrolases extracted from bacteria and other organisms.

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Metabolism of sodium oestrone [35S]sulphate in the rat

Cited by 14 publications

References 27 publications

Studies of the oestrogen sulphatase and arylsulphatase C activities of rat liver

Studies of the oestrogen sulphatase and arylsulphatase C activities of rat liver

Metabolism of Synthetic Steroids by Animals and Man

Liberation of sulfate from sulfate esters by soils

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