Metabolite changes in nine different soybean varieties grown under field and greenhouse conditions

John, K.M. Maria; Natarajan, Savithiry; Luthria, Devanand L.

doi:10.1016/j.foodchem.2016.05.055

Cited by 29 publications

(16 citation statements)

References 35 publications

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“…Similar results on isoflavones content (1.2–3.3 mg/g) were also reported by Wang and Murphy () on a single genotypes grown over multiple time periods. Both, MiniMax and Williams 82 genotypes grown under field condition showed lower content of individual isoflavones as compared to the samples grown under greenhouse (Maria John et al, ). Photoperiod and other controlled conditions in the greenhouse favored the isoflavones biosynthesis.…”

Section: Resultsmentioning

confidence: 97%

“…The primary metabolites were extracted and analyzed in six replicates by method previously reported (Maria John, Natarajan, & Luthria, ). In brief, ground soybeans (100 mg) were extracted with 2 mL of methanol : water : chloroform (2.5:1:1 v:v:v) by ultrasonication (10 min).…”

Section: Methodsmentioning

confidence: 99%

“…The temperature program begins from 170°C with an equilibration time of 3 min and finally reached to 215°C with the ramp of 1°C. The detector was maintained at 270°C with a hydrogen flow at 45 mL/min, air flow at 450 mL/min, and make up nitrogen flow at 45 mL/min (Maria John et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Mass spectra were obtained using electrospray ionization in positive and negative modes within a range of 300–1,200 m/z. The operating parameters were as follows: needle voltage is 3,500 volts; capillary voltage, 70 V; drying gas temperature, 350°C; drying gas pressure, 30 psi nitrogen (Maria John et al, ).…”

Section: Methodsmentioning

confidence: 99%

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Proteomic and metabolomic analysis of minimax and Williams 82 soybeans grown under two different conditions

et al. 2017

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MiniMax was developed as a model soybean for research because of its small stature, limited planting space, and rapid generation time. In the present study, the proteins, isoflavones, and fatty acid methyl esters (FAMEs) were compared between dwarf (MiniMax) and commercially cultivated (Williams 82) soybean genotypes under two growing conditions (greenhouse and field). The protein profile of the soybean extracts by 2D‐PAGE showed at least 16 of 716 spots that were differently expressed in MiniMax and Williams 82. These spots were primarily six proteins namely, seed biotin, β‐conglycinin, glycinin, seed‐maturation protein, glutathione‐S‐transferase, and sucrose binding protein. In both genotypes, higher content of isoflavone aglycones was observed in greenhouse grown samples as compared to the field grown samples. However, only field grown Willams 82 had higher levels of FAMEs as compared to other three groups. This study shows that both growing conditions and cultivars significantly influence the compositional content of metabolites. Practical applications The global demand of soybean and its products has increased due to its high protein and isoflavones content. Researchers are actively developing new genotypes with improved yields, greater drought and disease tolerance, and reduced growth time. MiniMax, a dwarf genotype of soybean showed early flowering, seed setting, and maturation traits due to its multiple dwarfing genes. This characteristic feature enables researchers to study effects of various treatments on soybean in a shorter period with multiple replications in a single growing season.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Proteomic and metabolomic analysis of minimax and Williams 82 soybeans grown under two different conditions

et al. 2017

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“…is an important crop that serves as a significant source of oil (~20%) and protein (~40%). The agricultural importance of soybean has been recognized owing to its various beneficial effects on human health (Oksman-Caldentey and Hiltunen, 1996;Zeng et al, 2004;Manavalan et al, 2009;John et al, 2016). Given its amenability to genetic transformation, soybean has been subjected to gene transfer.…”

Section: Introductionmentioning

confidence: 99%

Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

et al. 2020

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Soybean is a major crop that is used as a source of vegetable oil for human use. To develop transgenic soybean with high a-linolenic acid (ALA; 18:3) content, the FAD3-1 gene isolated from lesquerella (Physaria fendleri) was used to construct vectors with two different seed-specific promoters, soybean b-conglycinin (Pb-con) and kidney bean phaseolin (Pphas), and one constitutive cauliflower mosaic virus 35S promoter (P35S). The corresponding vectors were used for Agrobacterium-mediated transformation of imbibed mature half seeds. The transformation efficiency was approximately 2%, 1%, and 3% and 21, 7, and 17 transgenic plants were produced, respectively. T-DNA insertion and expression of the transgene were confirmed from most of the transgenic plants by polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), reverse transcription PCR (RT-PCR), and Southern blot analysis. The fatty acid composition of soybean seeds was analyzed by gas chromatography. The 18:3 content in the transgenic generation T 1 seeds was increased 7-fold in Pb-con:PfFAD3-1, 4-fold in Pphas : PfFAD3-1, and 1.6-fold in P35S:PfFAD3-1 compared to the 18:3 content in soybean "Kwangankong". The increased content of 18:3 in the Pb-con:PfFAD3-1 soybean (T 1 ) resulted in a 52.6% increase in total fatty acids, with a larger decrease in 18:1 content than 18:2 content. The increase in 18:3 content was also maintained and reached 42% in the Pphas : PfFAD3-1 transgenic generation T 2 . Investigations of the agronomic traits of 12 Pb-con:PfFAD3-1 transgenic lines (T 1 ) revealed that plant height, number of branches, nodes, pods, total seeds, and total seed weight were significantly higher in several transgenic lines than those in non-transgenic soybean. Especially, an increase in seed size was observed upon expression of the PfFAD3-1 gene with the b-conglycinin promoter, and 6%-14% higher seed lengths were measured from the transgenic lines.

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Protein plant factories: production and resource use efficiency of soybean proteins in vertical farming

Righini,

Graamans,

van Hoogdalem

et al. 2024

J Sci Food Agric

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BackgroundControlled environment agriculture (CEA), particularly vertical farms (VF), also called plant factories, is often claimed as a solution for global food security due to its ability to produce crops unaffected by weather or pests. In principle, essential macronutrients of the human diet, like protein, could technically be produced in VF. This aspect becomes relevant in the era of protein transition, marked by an increasing consumer interest in plant‐based protein and environmental challenges faced by conventional farming. However, the real question is: what does the cultivation of protein crops in VF imply in terms of resource use? To address this, a study was conducted using a VF experiment focusing on two soybean cultivars.ResultsWith a variable plant density to optimize area use, and thanks to the ability to have more crop cycles per year, protein yield per square meter crop was about 8 times higher than in the open field. Assuming soy as the only protein source in the diet, the resources needed to get total yearly protein requirement of a reference adult would be 20 m2 of crop area, 2.4 m3 of water, and 16 MWh of electricity, versus 164 m2, 111 m3 and 0.009 MWh in the field.ConclusionsThe study's results inform the debate on protein production and the efficiency of VF compared to conventional methods. With current electricity prices, it is unlikely to justify production of simple protein crops in VF or promote it as a solution to meet global protein needs.This article is protected by copyright. All rights reserved.

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Metabolite changes in nine different soybean varieties grown under field and greenhouse conditions

Cited by 29 publications

References 35 publications

Proteomic and metabolomic analysis of minimax and Williams 82 soybeans grown under two different conditions

Proteomic and metabolomic analysis of minimax and Williams 82 soybeans grown under two different conditions

Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

Protein plant factories: production and resource use efficiency of soybean proteins in vertical farming

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