Metabolite profile comparisons between ascending and descending colon tissue in healthy adults

Baxter, Bridget A.; Parker, Kristopher D.; Nosler, Michael J.; Rao, Sangeeta; Craig, R. Bruce; C, Seiler; Ryan, Elizabeth P.

doi:10.3748/wjg.v26.i3.335

Cited by 17 publications

(20 citation statements)

References 55 publications

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“…The selection of microbial communities with protection in the murine model may differ from human FMT utility due to a series of metabolic differences between mice and humans (e.g., the primary role for cecal fermentation in mice). We also appreciate larger differences between the proximal and distal colon metabolism of humans [ 58 ]. Comprehensive multi-omics analysis of the microbial inoculums used for FMT and the host system will further advance the amplicon/or marker gene-based studies [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation

et al. 2021

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Rice bran, removed from whole grain rice for white rice milling, has demonstrated efficacy for the control and suppression of colitis and colon cancer in multiple animal models. Dietary rice bran intake was shown to modify human stool metabolites as a result of modifications to metabolism by gut microbiota. In this study, human stool microbiota from colorectal cancer (CRC) survivors that consumed rice bran daily was examined by fecal microbiota transplantation (FMT) for protection from azoxymethane and dextran sodium sulfate (AOM/DSS) induced colon carcinogenesis in germ-free mice. Mice transfaunated with rice bran-modified microbiota communities (RMC) harbored fewer neoplastic lesions in the colon and displayed distinct enrichment of Flavonifractor and Oscillibacter associated with colon health, and the depletion of Parabacteroides distasonis correlated with increased tumor burden. Two anti-cancer metabolites, myristoylcarnitine and palmitoylcarnitine were increased in the colon of RMC transplanted mice. Trimethylamine-N-oxide (TMAO) and tartarate that are implicated in CRC development were reduced in murine colon tissue after FMT with rice bran-modified human microbiota. Findings from this study show that rice bran modified gut microbiota from humans confers protection from colon carcinogenesis in mice and suggests integrated dietary-FMT intervention strategies should be tested for colorectal cancer control, treatment, and prevention.

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Section: Discussionmentioning

confidence: 99%

Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation

et al. 2021

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“…The predominant functions of the gastrointestinal tract are digestion, absorption of nutrients, secretion, and immune response and control [ 28 ]. Differences in the metabolome of AC and DC colon tissue, including the profiles of nucleotides, amino acids, and lipids, have been described for healthy, overweight, and obese adults, respectively [ 29 ]. To our knowledge, no study comparing the proteomic profiles of colon tissue in AC, DC, and TV colon has currently been performed.…”

Section: Discussionmentioning

confidence: 99%

Tissue Sampling and Homogenization with NIRL Enables Spatially Resolved Cell Layer Specific Proteomic Analysis of the Murine Intestine

Voß

Moritz

Pelczar

et al. 2022

IJMS

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For investigating the molecular physiology and pathophysiology in organs, the most exact data should be obtained; if not, organ-specific cell lines are analyzed, or the whole organ is homogenized, followed by the analysis of its biomolecules. However, if the morphological organization of the organ can be addressed, then, in the best case, the composition of molecules in single cells of the target organ can be analyzed. Laser capture microdissection (LCM) is a technique which enables the selection of specific cells of a tissue for further analysis of their molecules. However, LCM is a time-consuming two-dimensional technique, and optimal results are only obtained if the tissue is fixed, e.g., by formalin. Especially for proteome analysis, formalin fixation reduced the number of identifiable proteins, and this is an additional drawback. Recently, it was demonstrated that sampling of fresh-frozen (non-fixed) tissue with an infrared-laser is giving higher yields with respect to the absolute protein amount and number of identifiable proteins than conventional mechanical homogenization of tissues. In this study, the applicability of the infrared laser tissue sampling for the proteome analysis of different cell layers of murine intestine was investigated, using LC–MS/MS-based differential quantitative bottom-up proteomics. By laser ablation, eight consecutive layers of colon tissue were obtained and analyzed. However, a clear distinguishability of protein profiles between ascending, descending, and transversal colon was made, and we identified the different intestinal-cell-layer proteins, which are cell-specific, as confirmed by data from the Human Protein Atlas. Thus, for the first time, sampling directly from intact fresh-frozen tissue with three-dimensional resolution is giving access to the different proteomes of different cell layers of colon tissue.

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“…Regional variation in the colon microbiota and its downstream metabolomic derivatives is evident in normal versus overweight and obese individuals. The ascending colon of obese adults may exhibit higher levels of the lipid metabolite trimethylamine-N-oxide and of primary (chenoxycholate) and secondary (taurodeoxycholate) bile acids, but a lower abundance of certain endocannabinoids [136]. This metabolic profile is linked to the overconsumption of certain foods and is conducive to the induction of colon inflammation [136,146].…”

Section: Intestinal Metabolomics As An Emerging Way To Study Crc Prev...mentioning

confidence: 99%

Colon Cancer: From Epidemiology to Prevention

Katsaounou

Nicolaou

Vogazianos³

et al. 2022

Metabolites

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Colorectal cancer (CRC) is one of the most prevalent cancers affecting humans, with a complex genetic and environmental aetiology. Unlike cancers with known environmental, heritable, or sex-linked causes, sporadic CRC is hard to foresee and has no molecular biomarkers of risk in clinical use. One in twenty CRC cases presents with an established heritable component. The remaining cases are sporadic and associated with partially obscure genetic, epigenetic, regenerative, microbiological, dietary, and lifestyle factors. To tackle this complexity, we should improve the practice of colonoscopy, which is recommended uniformly beyond a certain age, to include an assessment of biomarkers indicative of individual CRC risk. Ideally, such biomarkers will be causal to the disease and potentially modifiable upon dietary or therapeutic interventions. Multi-omics analysis, including transcriptional, epigenetic as well as metagenomic, and metabolomic profiles, are urgently required to provide data for risk analyses. The aim of this article is to provide a perspective on the multifactorial derailment of homeostasis leading to the initiation of CRC, which may be explored via multi-omics and Gut-on-Chip analysis to identify much-needed predictive biomarkers.

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Metabolite profile comparisons between ascending and descending colon tissue in healthy adults

Cited by 17 publications

References 55 publications

Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation

Dietary Rice Bran-Modified Human Gut Microbial Consortia Confers Protection against Colon Carcinogenesis Following Fecal Transfaunation

Tissue Sampling and Homogenization with NIRL Enables Spatially Resolved Cell Layer Specific Proteomic Analysis of the Murine Intestine

Colon Cancer: From Epidemiology to Prevention

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