Metabolite variation in three edible Italian Allium cepa L. by NMR-based metabolomics: a comparative study in fresh and stored bulbs

Saviano, Gabriella; Paris, Debora; Melck, Dominique; Fantasma, Francesca; Motta, Andréa; Iorizzi, Maria

doi:10.1007/s11306-019-1566-6

Cited by 27 publications

(17 citation statements)

References 36 publications

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“…Metabolomics analysis revealed that sucrose makes up the greatest proportion of sugar and other chemical compounds of shallot (Figure 6). This result agrees with the findings of Saviano et al (2019), who revealed that 80% of the chemical compounds accumulated in onion bulbs are composed of carbohydrates, such as glucose, sucrose, and fructans. Sucrose is also the most abundant soluble carbohydrate in most onion cultivars (Pöhnl et al 2018).…”

Section: Carbohydratessupporting

confidence: 93%

“…The spectra were processed by manual phasing, baseline correction, and calibration to internal standard solution signals (TMSP) placement at the chemical shift of 0.0 ppm. Metabolites were identified by comparison with the data in a metabolite database as described in previous research (Kim et al 2010;Jo et al 2020;Ritota et al 2012;Saviano et al 2019). Semi-quantitatively signal analysis was performed by comparing the area of a signal to that of the corresponding internal standard.…”

Section: Discussionmentioning

confidence: 99%

“…Holmes et al (2006) and Jahangir et al (2018) reported that NMR metabolic profiling can characterize and identify different metabolite profiles at the tissue and even cell level within the same plant. NMR metabolite profiling of Allium species has been conducted for garlic and onion (Jo et al 2020;Saviano et al 2019) but not shallot bulb. Thus, the present study was designed to collect the metabolite information of two shallot cultivars possessing different morphological characteristics.…”

Section: Introductionmentioning

confidence: 99%

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Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance

et al. 2021

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Abstract. Basundari FRA, Sulistyaningsih E, Murti RH, Nuringtyas TR. 2021. Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance. Biodiversitas 22: 3127-3135. Shallot, an Allium species with high economic value, is widely cultivated in Indonesia. The species includes numerous cultivars with unique characteristics, such as harvesting time, yield, taste, odor, and bulb color. Tuk Tuk and Trisula, the cultivars used in this experiment, have different bulb colors. Tuk Tuk’s bulb color is light red, while that of Trisula is dark red. This color difference may be attributed to variations in the metabolite contents of the shallot bulbs. The present experiment aimed to reveal differences in the metabolite contents of the two shallot cultivars by using Nuclear Magnetic Resonance (NMR) and then determine the specific metabolites contributing to these differences. Bulbs of Tuk Tuk and Trisula were planted in the field under the same conditions and then collected. The bulb samples were powdered, freeze-dried, and subjected to a two-phase extraction method with CDCl3 and D2O as solvents to separate the nonpolar metabolites from polar ones. The extracts were analyzed by 1H-NMR, and the spectra collected were analyzed using MNOVA software and Metaboanalyst.ca. A total of 23 metabolites were successfully identified and characterized in this experiment. The contents of eight of these metabolites, namely, sucrose, glutamine, citric acid, choline, methiin, propiin, threonine, and formic acid, were significantly higher in Trisula than in Tuk Tuk. These differences may be correlated with variations in the color intensity, pungency, and other traits of the cultivars. The results demonstrate that NMR metabolite profiling could effectively differentiate metabolite profile variations among shallot cultivars.

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Section: Carbohydratessupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance

et al. 2021

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“…Among alliums, metabolic profiling is so far performed in onion bulbs, considering cultivars and cultivation year ( Böttcher et al, 2018 ) ; change in organosulfur metabolites after heat treatment in onion ( Kim et al, 2016 ); differentiation profiling of black and fresh garlics ( Molina-Calle et al, 2017 ) ; low-temperature conditioning of “seed” cloves ( Dufoo-Hurtado et al, 2013 ) ; volatile profiling of onion and garlic after subjecting to different drying methods ( Farag et al, 2017 ) ; metabolic profiling of leaves, bulbs, roots, and basal stems in A. roylei and A. porrum L. ( Abdelrahman et al, 2014 ; Soininen et al, 2014a ; Soininen et al, 2012 ); authentication of the geographic origin of garlic ( Hrbek et al, 2018 ) ; bulb color and flavonoids ( Zhang et al, 2018 ; Abdelrahman et al, 2019 ) ; and metabolic profiling of fresh and stored onion bulbs ( Saviano et al, 2019 ) . Metabolomics, along with the artificial neural network, was used for sensory evaluation of garlic and garlic products by using 89 quality indicators ( Liu et al, 2019 ) .…”

Section: Survey Methodologymentioning

confidence: 99%

Omics approaches inAlliumresearch: Progress and way ahead

Khandagale

Krishna

Roylawar

et al. 2020

PeerJ

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Background The genus Allium (Family: Amaryllidaceae) is an economically important group of crops cultivated worldwide for their use as a vegetable and spices. Alliums are also well known for their nutraceutical properties. Among alliums, onion, garlic, leek, and chives cultivated worldwide. Despite their substantial economic and medicinal importance, the genome sequence of any of the Allium is not available, probably due to their large genome sizes. Recently evolved omics technologies are highly efficient and robust in elucidating molecular mechanisms of several complex life processes in plants. Omics technologies, such as genomics, transcriptomics, proteomics, metabolomics, metagenomics, etc. have the potential to open new avenues in research and improvement of allium crops where genome sequence information is limited. A significant amount of data has been generated using these technologies for various Allium species; it will help in understanding the key traits in Allium crops such as flowering, bulb development, flavonoid biosynthesis, male sterility and stress tolerance at molecular and metabolite level. This information will ultimately assist us in speeding up the breeding in Allium crops. Method In the present review, major omics approaches, and their progress, as well as potential applications in Allium crops, could be discussed in detail. Results Here, we have discussed the recent progress made in Allium research using omics technologies such as genomics, transcriptomics, micro RNAs, proteomics, metabolomics, and metagenomics. These omics interventions have been used in alliums for marker discovery, the study of the biotic and abiotic stress response, male sterility, organ development, flavonoid and bulb color, micro RNA discovery, and microbiome associated with Allium crops. Further, we also emphasized the integrated use of these omics platforms for a better understanding of the complex molecular mechanisms to speed up the breeding programs for better cultivars. Conclusion All the information and literature provided in the present review throws light on the progress and potential of omics platforms in the research of Allium crops. We also mentioned a few research areas in Allium crops that need to be explored using omics technologies to get more insight. Overall, alliums are an under-studied group of plants, and thus, there is tremendous scope and need for research in Allium species.

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“…NMR based metabolomics is also very useful in simultaneously monitoring different level of possible variation in nutraceutical composition, as shown by a study comparing three different cultivars of onion (Allium cepa L.) typically cultivated in Italy [94]. The authors were able to build a model based on NMR fingerprints to discriminate the three A. cepa cultivars.…”

Section: Figurementioning

confidence: 99%

NMR-Based Plant Metabolomics in Nutraceutical Research: An Overview

et al. 2020

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Few topics are able to channel the interest of researchers, the public, and industries, like nutraceuticals. The ever-increasing demand of new compounds or new sources of known active compounds, along with the need of a better knowledge about their effectiveness, mode of action, safety, etc., led to a significant effort towards the development of analytical approaches able to answer the many questions related to this topic. Therefore, the application of cutting edges approaches to this area has been observed. Among these approaches, metabolomics is a key player. Herewith, the applications of NMR-based metabolomics to nutraceutical research are discussed: after a brief overview of the analytical workflow, the use of NMR-based metabolomics to the search for new compounds or new sources of known nutraceuticals are reviewed. Then, possible applications for quality control and nutraceutical optimization are suggested. Finally, the use of NMR-based metabolomics to study the impact of nutraceuticals on human metabolism is discussed.

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Metabolite variation in three edible Italian Allium cepa L. by NMR-based metabolomics: a comparative study in fresh and stored bulbs

Cited by 27 publications

References 36 publications

Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance

Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance

Omics approaches inAlliumresearch: Progress and way ahead

NMR-Based Plant Metabolomics in Nutraceutical Research: An Overview

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