Metabolomic analyses to evaluate the effect of drought stress on selected African Eggplant accessions

Mibei, Elias Kibiwot; Owino, Willis; Ambuko, Jane; Giovannoni, James J.; Onyango, Arnold N.

doi:10.1002/jsfa.8458

Cited by 42 publications

(18 citation statements)

References 34 publications

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“…Apart from proline, several other amino acids were clearly induced in YN211 but not in HX10. Many amino acids have been shown in response to the water/drought stress either at a higher or lower level [45,46]. Proline is one of the main osmolytes in plants and could accumulate in response to various abiotic stresses [47].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Response for Drought Stress Tolerance in Chinese Wheat Genotypes (Triticum aestivum)

Guo

Xin

Tie-gang

et al. 2020

Plants

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Metabolomics is an effective biotechnological tool that can be used to attain comprehensive information on metabolites. In this study, the profiles of metabolites produced by wheat seedlings in response to drought stress were investigated using an untargeted approach with ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to determine various physiological processes related to drought tolerance from the cross between drought-tolerant genotype (HX10) and drought-sensitive genotype (YN211). The current study results showed that under drought stress, HX10 exhibited higher growth indices than YN211. After drought stress treatment, a series of phenolics accumulated higher in HX10 than in YN211, whereas the amount of thymine, a pyrimidine, is almost 13 folds of that in YN211. These metabolites, as well as high levels of different amino acids, alkaloids, organic acids, and flavonoids in the drought treated HX10 could help to explain its strong drought-tolerant capacity. The current study explored the understanding of the mechanisms involved in the drought response of wheat seedling; these metabolome data could also be used for potential QTL or GWAS studies to identify locus (loci) or gene(s) associated with these metabolic traits for the crop improvement.

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Section: Discussionmentioning

confidence: 99%

Metabolomics Response for Drought Stress Tolerance in Chinese Wheat Genotypes (Triticum aestivum)

Guo

Xin

Tie-gang

et al. 2020

Plants

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“…The TCA- cycle regulates the synthesis of several amino acids [83]. Higher amount of glutamic acid, GABA and threonine was found in Soissons, Žitarka, Toborzó and Antonija under drought as compared to Srpanjka and Ellvis.…”

Section: Discussionmentioning

confidence: 99%

Metabolic response to drought in six winter wheat genotypes

et al. 2019

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Wheat is one of the most important cereals, whose growth and development is strongly limited by drought. This study investigated the physiological and metabolic response of six winter wheat cultivars to drought with the emphasis on the induction of dominant metabolites affected by the treatment and genotypes or both. The plants were exposed to a moderate (non-lethal) drought stress, which was induced by withholding watering for six days under controlled greenhouse conditions. A decline in CO 2 assimilation (Pn) and transpiration rate, stomata closure, a decrease in relative water content (RWC) and increase of malondialdehyde content were observed in drought-treated plants of all cultivars. These changes were most pronounced in Ellvis, while Soissons was able to retain the higher RWC and Pn. Among the studied metabolites, sugars (sucrose, glucose, fructose, several disaccharides), organic acids (malic acid, oxalic acids), amino acids (proline, threonine, gamma-aminobutyric acid (GABA), glutamine) and sugar alcohols such as myo-inositol accumulated to higher levels in the plants exposed to drought stress in comparison with the control. The accumulation of several metabolites in response to drought differed between the genotypes. Drought induced the production of sucrose, malic acid and oxalic acid, unknown organic acid 1, unknown disaccharide 1, 2 and 3, GABA, L-threonine, glutamic acid in four (Soissons, Žitarka, Antonija or Toborzó) out of six genotypes. In addition, Soissons, which was the most drought tolerant genotype, accumulated the highest amount of unknown disaccharide 5, galactonic and phosphoric acids. The two most drought sensitive cultivars, Srpanjka and Ellvis, demonstrated different metabolic adjustment in response to the stress treatment. Srpanjka responded to drought by increasing the amount of glucose and fructose originated from hydrolyses of sucrose and accumulating unidentified sugar alcohols 1 and 2. In Ellvis, drought caused inhibition of photosynthetic carbon metabolism, as evidence by the decreased Pn, gs, RWC and accumulation levels of sugar metabolites (sucrose, glucose and fructose). The results revealed the differences in metabolic response to drought among the genotypes, which drew attention on metabolites related with general response and on those metabolites which are part of specific response that may play an important role in drought tolerance.

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“…On the other hand, proline has been reported to be the most abundant osmolyte accumulated in response to multiple environmental stresses including water stress and pathogen infection, which acts as an ROS scavenger as well ( De Ronde et al, 2000 ; Hanci and Cebeci, 2014 ; Rodziewicz et al, 2014 ). Recent findings have demonstrated that water stressed eggplants accumulated osmoprotectants like proline, as well as glucose and fructose and this phenomenon has been correlated with increased tolerance toward water deficit and prevention from wilting ( Mibei et al, 2018 ). Our results showed significant difference between control plants and water stressed or V. dahliae infected plants in proline concentration, however, no significant difference was observed between control plants and plants under combined stress for the Skoutari cultivar.…”

Section: Discussionmentioning

confidence: 99%

Cultivar-Dependent Responses of Eggplant (Solanum melongena L.) to Simultaneous Verticillium dahliae Infection and Drought

et al. 2018

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Several studies regarding the imposition of stresses simultaneously in plants have shown that plant responses are different under individual and combined stress. Pathogen infection in combination with drought can act both additively and antagonistically, suggesting a tailored-made plant response to these stresses. The aforementioned combination of stresses can be considered as one of the most important factors affecting global crop production. In the present research we studied eggplant responses to simultaneous Verticillium dahliae infection and drought with respect to the application of the individual stresses alone and investigated the extent to which these responses were cultivar dependent. Two eggplant cultivars (Skoutari and EMI) with intermediate resistance to V. dahliae were subjected to combined stress for a 3-week period. Significant differences in plant growth, several physiological and biochemical parameters (photosynthesis rate, leaf gas exchanges, Malondialdehyde, Proline) and gene expression, were found between plants subjected to combined and individual stresses. Furthermore, plant growth and molecular (lipid peroxidation, hydrogen peroxide, gene expression levels) changes highlight a clear discrimination between the two cultivars in response to simultaneous V. dahliae infection and drought. Our results showed that combined stress affects significantly plants responses compared to the application of individual stresses alone and that these responses are cultivar dependent.

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Metabolomic analyses to evaluate the effect of drought stress on selected African Eggplant accessions

Cited by 42 publications

References 34 publications

Metabolomics Response for Drought Stress Tolerance in Chinese Wheat Genotypes (Triticum aestivum)

Metabolomics Response for Drought Stress Tolerance in Chinese Wheat Genotypes (Triticum aestivum)

Metabolic response to drought in six winter wheat genotypes

Cultivar-Dependent Responses of Eggplant (Solanum melongena L.) to Simultaneous Verticillium dahliae Infection and Drought

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