Metabolomic and transcriptomic analysis of roots of tobacco varieties resistant and susceptible to bacterial wilt

Shi, Haoqi; Xu, Peiwen; Yu, Wen Mei; Cheng, Yazhi; Ding, Anming; Wang, Weifeng; Wu, Shengxin; Sun, Yuhe

doi:10.1016/j.ygeno.2022.110471

Cited by 12 publications

(15 citation statements)

References 57 publications

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“…Many secondary metabolites, such as flavonoids 28 , phenolic compounds 29 , 30 , coumarins 31 – 33 , among others, have been shown to have the ability to shape the composition of microbial communities. In our tentative published data, KCB-1 and CB-1 exhibit different metabolic reprogramming of root metabolites after inoculation with R. solanacearum 34 . We focused on coumarins.…”

Section: Discussionmentioning

confidence: 60%

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Shi

et al. 2022

Sci Rep

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Tobacco bacterial wilt has seriously affected tobacco production. Ethyl methanesulfonate (EMS) induced tobacco bacterial wilt resistant mutants are important for the control of tobacco bacterial wilt. High-throughput sequencing technology was used to study the rhizosphere bacterial community assemblages of bacterial wilt resistant mutant tobacco rhizosphere soil (namely KS), bacterial wilt susceptible tobacco rhizosphere soil (namely GS) and bulk soil (namely BS) in Xuancheng, Huanxi, Yibin and Luzhou. Alpha analysis showed that the bacterial community diversity and richness of KS and GS in the four regions were not significantly different. However, analysis of intergroup variation in the top 15 bacterial communities in terms of abundance showed that the bacterial communities of KS and GS were significantly different from BS, respectively. In addition, pH, alkali-hydrolysable nitrogen (AN) and soil organic carbon (SOC) were positively correlated with the bacterial community of KS and negatively correlated with GS in the other three regions except Huanxi. Network analysis showed that the three soils in the four regions did not show a consistent pattern of network complexity. PICRUSt functional prediction analysis showed that the COG functions were similar in all samples. All colonies were involved in RNA processing and modification, chromatin structure and dynamics, etc. In conclusion, our experiments showed that rhizosphere bacterial communities of tobacco in different regions have different compositional patterns, which are strongly related to soil factors.

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Section: Discussionmentioning

confidence: 60%

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Shi

et al. 2022

Sci Rep

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Section: Discussionmentioning

confidence: 80%

“…Previous metabolomic analyses demonstrate a consistent correlation between tomato bacterial wilt resistance and both preformed and induced phenolic compounds, notably trans-cinnamic acids (Shi et al 2022; Zeiss et al 2022; Chen et al 2021; Zeiss et al 2019; Zeiss et al 2018) add refs A recent physico-chemical study showed that infection of H7996 with Ralstonia GMI1000 triggered increased deposition of ligno-suberin and hydroxycinnamic acid derivatives on vessel walls, thereby restricting bacterial colonization (Kashyap 2022). Moreover, transgenically upregulating ligno-suberin production slowed disease progress on susceptible cv.…”

Section: Discussionmentioning

confidence: 91%

Ralstonia solanacearumpandemic lineage strain UW551 overcomes inhibitory xylem chemistry to break tomato bacterial wilt resistance

Hamilton

Zaricor

Dye

et al. 2023

Preprint

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Plant pathogenic Ralstonia strains cause bacterial wilt disease by colonizing xylem vessels of many crops, including tomato. Host resistance is the best control for bacterial wilt, but resistance mechanisms of the widely used Hawaii7996 tomato breeding line are unknown. Using growth in ex vivo xylem sap as a proxy, we found that Ralstonia strain GMI1000 grows in sap from both healthy plants and Ralstonia-infected susceptible plants. However, sap from Ralstonia-infected Hawaii7996 plants inhibited Ralstonia growth, suggesting that in response to Ralstonia infection, resistant plants increase inhibitors in their xylem sap. Consistent with this, reciprocal grafting and defense gene expression experiments indicated that Hawaii7996 wilt resistance acts both above- and belowground. Concerningly, Hawaii7996 resistance is broken by Ralstonia strain UW551 of the pandemic lineage that threatens highland tropical agriculture. Unlike other Ralstonia strains, UW551 grew well in sap from Ralstonia-infected Hawaii7996 plants. Moreover, other Ralstonia strains could grow in sap from Hawaii7996 plants previously infected by UW551. Thus, UW551 overcomes Hawaii7996 resistance in part by detoxifying inhibitors in xylem sap. Testing a panel of xylem sap compounds identified by metabolomics revealed that no single chemical differentially inhibits Ralstonia strains that cannot infect Hawaii7996. However, sap from Ralstonia-infected Hawaii7996 contained more phenolic compounds, which are known plant antimicrobial defenses. Culturing UW551 in this sap reduced total phenolic levels, indicating that the resistance-breaking Ralstonia strain degrades these chemical defenses. Together, these results suggest that Hawaii7996 tomato wilt resistance depends at least in part on inducible phenolic compounds in xylem sap.

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“…3 Tobacco, as a significant cash crop, is susceptible to various phytopathogen infestations throughout its growth cycle. 4 For a considerable period of time, chemical pesticides have been extensively employed to prevent and manage plant diseases. However, issues such as pathogen resistance, pesticide residues, and environmental contamination have arisen.…”

Section: Introductionmentioning

confidence: 99%

“…A multitude of factors contribute to the uneven distribution of food worldwide, among which biotic stress caused by fungal, viral, and bacterial pathogens poses a significant constraint on production 3 . Tobacco, as a significant cash crop, is susceptible to various phytopathogen infestations throughout its growth cycle 4 . For a considerable period of time, chemical pesticides have been extensively employed to prevent and manage plant diseases.…”

Section: Introductionmentioning

confidence: 99%

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

Cai,

Huang,

Feng

et al. 2023

Pest Management Science

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The utilization of nonmetallic inorganic nanomaterials for antimicrobial photocatalytic technology has emerged as a promising approach to combat drug‐resistant bacteria. Recently, g‐C3N4 nanosheets have attracted significant attention due to their exceptional stability, degradability, low cost, and remarkable antibacterial properties. In this study, a facile electrostatic self‐assembly approach was utilized to functionalize ZnO nanoparticles with g‐C3N4 nanosheets, resulting in the formation of g‐C3N4@ZnO nanoparticle composites. The Z‐shaped heterojunction architecture of these composites facilitates efficient separation of photogenerated electron‐hole pairs and enhances visible light catalytic performance. Moreover, the formation of the g‐C3N4@ZnO heterostructure showed a higher photocatalytic capacity and the generation of reactive oxygen species than g‐C3N4 nanosheets. The photocatalytic antibacterial mechanisms of g‐C3N4@ZnO at the transcriptomic level primarily involve disrupting bacterial membrane synthesis and inhibiting motility and energy metabolism. Therefore, the antibacterial mechanism of g‐C3N4@ZnO can be attributed to a combination of physical membrane damage, chemical damage (ROS enhancement) and inhibition of chemotaxis, biofilm formation and flagellar motility. These findings collectively provide novel high potential and insights into the practical application of photocatalysts in plant disease management.This article is protected by copyright. All rights reserved.

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Metabolomic and transcriptomic analysis of roots of tobacco varieties resistant and susceptible to bacterial wilt

Cited by 12 publications

References 57 publications

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Ralstonia solanacearumpandemic lineage strain UW551 overcomes inhibitory xylem chemistry to break tomato bacterial wilt resistance

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

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Metabolomic and transcriptomic analysis of roots of tobacco varieties resistant and susceptible to bacterial wilt

Cited by 12 publications

References 57 publications

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Ralstonia solanacearumpandemic lineage strain UW551 overcomes inhibitory xylem chemistry to break tomato bacterial wilt resistance

Composite g‐C3N4@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease

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Product

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About

Composite g‐C₃N₄@ZnO NP electrostatic self‐assembly: enhanced ROS as a key factor for high‐efficiency control of tobacco wildfire disease