Metabolomic characterization of 5 native Peruvian chili peppers (Capsicum spp.) as a tool for species discrimination

Espichán, Fabio; Rojas, Rosario; Quispe, Fredy; Cabanac, Guillaume; Marti, Guillaume

doi:10.1016/j.foodchem.2022.132704

Cited by 20 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values of R 2 Y and Q 2 greater than 0.7 indicated that the fit and prediction ability of this model was good. 14,23,28 These results implied that untargeted metabolomics analysis based on LC-MS/MS was suitable for identifying different type of O. gracilis cultures.…”

Section: Pca and Opls-da Analysis Of O Gracilis Mycelia Fermentation ...mentioning

confidence: 81%

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Wang

Yang

Tong

et al. 2023

Phytochemical Analysis

View full text Add to dashboard Cite

IntroductionOphiocordyceps gracilis, a type of edible and medicinal fungus, exhibits multiple health‐promoting effects. Due to the scarcity of natural O. gracilis, artificial cultures have been developed as its substitutes. However, lacking comprehension of the metabolite composition of cultures limits its utilisation.ObjectiveThis research aimed to evaluate the nutritional and medicinal value of four cultures of O. gracilis by analysing their metabolite composition. In addition, metabolic pathways in mycelia and fruiting bodies were analysed to explore fruiting body formation mechanism at metabolic level.MethodThe mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis were cultivated in this study. Their metabolite composition was compared using an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry (LC–MS/MS).ResultsPrincipal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS‐DA) showed that the four cultures have noticeable differences in metabolite composition. A total of 612 metabolites were identified, among which 159 metabolites showed significant differences, and these differential metabolites were classified into 13 categories. The metabolites in the fruiting bodies were the most abundant compared with other cultures. However, each culture had its own advantages and significantly accumulates some active metabolites respectively. Pearson's correlation analysed the mutual relationship among metabolites. In addition, seven metabolic pathways were closely related to fruiting body formation, such as “Biosynthesis of plant secondary metabolites”, “amino acids metabolism”, “tricarboxylic acid (TCA) cycle”.ConclusionThis study offered a reference to mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis as health‐promoting functional foods and medicine.

show abstract

Section: Pca and Opls-da Analysis Of O Gracilis Mycelia Fermentation ...mentioning

confidence: 81%

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Wang

Yang

Tong

et al. 2023

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

“…In this study, the OPLS-DA models after the pretreatment method (Par-scaling) revealed the best classification result [ 41 ]. Generally, three OPLS-DA score plots of the tested samples from three regions were sufficiently fit and predictive, since both of them had satisfied the conditions that the R 2 Y(cum) and Q 2 (cum) values were greater than 0.5 [ 42 ]. Specifically, R 2 Y(cum) = 0.994 and Q 2 (cum) = 0.984 in the extracts of Elian No.…”

Section: Resultsmentioning

confidence: 99%

Comparative Analysis of the Physicochemical Properties and Metabolites of Farinose and Crisp Lotus Roots (Nelumbo nucifera Gaertn.) with Different Geographical Origins

Liu

Peng

Yang

et al. 2023

Foods

View full text Add to dashboard Cite

Lotus roots are widely consumed vegetables because of their great taste and abundant nutrients, but their quality varies with the environments and cultivar. This study systematically compared farinose (Elian No. 5) and crisp (Elian No. 6) lotus root cultivars from three geographical origins. Pasting and texture characteristics verified that Elian No. 5 possessed lower hardness and lower ability to withstand shear stress and heating during cooking compared with Elian No. 6. Untargeted metabolite profiling was first performed using ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) combined with a Zeno trap. In total, 188 metabolites were identified based on the matching chemistry database. Multivariate analysis demonstrated that lotus roots from different cultivars and origins could be adequately distinguished. Sixty-one differential metabolites were identified among three Elian No. 5 samples, and 28 were identified among three Elian No. 6 samples. Isoscopoletin, scopoletin, and paprazine were the most differential metabolites between Elian No. 5 and Elian No. 6. These results can inform future research on the discrimination and utilization of lotus roots.

show abstract

“…Capsaicinoid and flavonoid contents, for example, were recently used as biochemical markers to distinguish Jize from Korean chile peppers [ 34 ]. In another study, an untargeted metabolomics approach identified biomarkers, including flavonoids, steroids, terpenoids, anthocyanin, and carboxylic acids, and characterized three Capsicum species ( C. baccatum, C. chinense , and C. frutescens ) from a collection of native South American chile peppers [ 35 ]. Eleven Mexican C. annuum cultivars were differentiated based on their metabolomic fingerprints, which consisted of sugars, amino acids, organic acids, polyphenolic acids, and alcohols [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Phenolic acids are among the most commonly found compounds in Capsicum fruits and have been observed to possess vital functions in human health, nutrition, and the prevention of diseases because of their antioxidant, antiviral, and antibacterial properties [ 5 , 39 ]. Phenolics have been previously identified in different species of peppers, including C. annuum cultivars from India [ 40 ]; C. annuum , C. chinense , and C. baccatum cultivated in New Mexico [ 41 ]; C. baccatum [ 42 ]; and C. frutescens and C. chinense cultivars from Peru, South America [ 35 ]. The human body cannot produce phenolic compounds; hence, they must be acquired from food sources [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

Widely Targeted Metabolomics Reveals Metabolite Diversity in Jalapeño and Serrano Chile Peppers (Capsicum annuum L.)

2023

View full text Add to dashboard Cite

Chile peppers (Capsicum annuum L.) are good sources of vitamins and minerals that can be included in the diet to mitigate nutritional deficiencies. Metabolomics examines the metabolites involved in biological pathways to understand the genes related to complex phenotypes such as the nutritional quality traits. The current study surveys the different metabolites present in jalapeño (‘NuMex Pumpkin Spice’) and serrano (‘NuMex LotaLutein’) type chile peppers grown in New Mexico using a widely targeted metabolomics approach, with the ‘NuMex LotaLutein’ as control. A total of 1088 different metabolites were detected, where 345 metabolites were differentially expressed; 203 (59%) were downregulated and 142 (41%) were upregulated (i.e., relative metabolite content is higher in ‘NuMex Pumpkin Spice’). The upregulated metabolites comprised mostly of phenolic acids (42), flavonoids (22), and organic acids (13). Analyses of principal component (PC) and orthogonal partial least squares demonstrated clustering based on cultivars, where at least 60% of variation was attributed to the first two PCs. Pathway annotation identified 89 metabolites which are involved in metabolic pathways and the biosynthesis of secondary metabolites. Altogether, metabolomics provided insights into the different metabolites present which can be targeted for breeding and selection towards the improvement of nutritional quality traits in Capsicum.

show abstract

Metabolomic characterization of 5 native Peruvian chili peppers (Capsicum spp.) as a tool for species discrimination

Cited by 20 publications

References 39 publications

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Comparative Analysis of the Physicochemical Properties and Metabolites of Farinose and Crisp Lotus Roots (Nelumbo nucifera Gaertn.) with Different Geographical Origins

Widely Targeted Metabolomics Reveals Metabolite Diversity in Jalapeño and Serrano Chile Peppers (Capsicum annuum L.)

Contact Info

Product

Resources

About