Metabolomic navigated Citrus waste repurposing to restore amino acids disorder in neural lesion

Zhang, Xiaoyue; Zhu, Zhaoju; Ren, Sipei; Deng, Yuchan; Xu, Jianing; Zhang, Si-Meng; Gao, Jin‐Ming; Zhang, Qiang

doi:10.1016/j.foodchem.2022.132933

Cited by 12 publications

(9 citation statements)

References 40 publications

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“…The parameter settings were according to the method we reported before. 11 Separation of the samples (5.0 μL) was carried out using a ReproSil-Pur Basic C 18 column (150 × 2.0 mm, 3.0 μm, Dr Maisch GmbH, Beim Brueckle, Germany) at 30 °C. Mobile phase A contained water with 0.1% (v/v) acetic acid and mobile phase B comprised acetonitrile with 0.1% (v/v) acetic acid.…”

Section: Methodsmentioning

confidence: 99%

Cyclocodon lancifoliusfruit prolongs the lifespan ofCaenorhabditis elegans viaantioxidation and regulation of purine metabolism

Liu,

Zhang,

Wang

et al. 2024

Food Funct.

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Section: Methodsmentioning

confidence: 99%

Cyclocodon lancifoliusfruit prolongs the lifespan ofCaenorhabditis elegans viaantioxidation and regulation of purine metabolism

Liu,

Zhang,

Wang

et al. 2024

Food Funct.

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“…Apoptotic neurons in zebrafish larvae were induced following a reported procedure [ 51 ]. In brief, the 4-dpf zebrafish larvae were treated with puerarin (40 μM) or lotusine (20 μM, 40 μM, 60 μM and 80 μM) for 24 h. Then the larvae were exposed to 1% alcohol in E3 water for 36 h. Then, the larvae were washed twice with PBS and incubated with 5 mg/L acridine orange (AO) in the dark for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS

Zhang

Yan

et al. 2022

IJMS

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Alcohol is an essential element in human culture. However, alcoholism has contributed to numerous health issues, including alcoholic fatty liver and sudden death. We found that the alkaloid lotusine possessed hepato- and neuroprotection against alcohol injuries. Lotusine showed comparable protective effects to puerarin, a widely recognized antagonist against alcohol damage. To better understand the metabolic response to alcohol injury and antagonist molecules, we applied sensitive zebrafish and LC-ESI-MS to collect metabolites related to alcohol, puerarin and lotusine exposure. LC-MS identified 119 metabolites with important physiological roles. Differential metabolomic analysis showed that alcohol caused abnormal expression of 82 metabolites (60 up-regulated and 22 down-regulated). These differential metabolites involved 18 metabolic pathways and modules, including apoptosis, necroptosis, nucleotide and fatty acid metabolism. Puerarin reversed seven metabolite variations induced by alcohol, which were related to necroptosis and sphingolipid metabolism. Lotusine was found to repair five metabolites disorders invoked by alcohol, mainly through nucleotide metabolism and glutathione metabolism. In phenotypic bioassay, lotusine showed similar activities to puerarin in alleviating behavioral abnormalities, neuroapoptosis and hepatic lipid accumulation induced by alcohol exposure. Our findings provided a new antagonist, lotusine, for alcohol-induced damage and explored the roles in repairing abnormal metabolism.

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“…The Institutional Animal Care & Use Committee of Northwest A&F University approved the animal experimental protocol (Protocol Permit Number: XN2023-0902, 15 March 2022). The investigation of zebra sh mobility was conducted in accordance with the previously established procedure outlined by our research group 37 . The zebra sh larvae at 5 dpf were subjected to treatment with varying quantities (20 µg/ml and 80 µg/ml) of WGE, PFL, and SFL samples for 4 hours.…”

Section: Zebra Sh Locomotor Behavior Investigationmentioning

confidence: 99%

“…Three portions of WGE (WGE1, WGE2, WGE3) and PFL (PFL 1, PFL2, PFL3) SFL (SFL 1, SFL2, SFL3) freeze-dried samples were precisely weighed and were respectively dissolved in MeOH solution to prepare the sample solution with the concentration of 100 μg/mL. UPLC-ESI-MS analysis and metabolite extraction procedures were conducted using the protocols outlined in our previously published works 37 . Furthermore, the PLS-DA analysis was performed using the Metaboanalyst website (https://www.metaboanalyst.ca/, visited on July 2, 2023).…”

Section: Metabolite Extraction and Lc-ms Analysis Of Ge At Different ...mentioning

confidence: 99%

Sleep-promotion of probiotics-fermented Gastrodia elata Bl.: Chemical variation and omics exploration.

Zhang,

zhang,

zhang

et al. 2024

Preprint

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Fermentation of Chinese herbal medicines (CHM) could enhance its bioactivities. We hypothesized probiotic-fermented GE with better potential to alleviate insomnia than thatof unfermented, thus the changes in chemical composition and the insomnia-alleviating effects and mechanisms of fermented GE on PTZ-induced insomnia zebrafish were explored via HPLC and LCMS, phenotypic, transcriptomic, and metabolomics analysis. The results demonstrated that probiotic fermented GE performed better than unfermented GE in increasing the content of chemical composition, reducing the displacement, average speed, and number of apoptotic cells in zebrafish with insomnia. Metabolomic investigation showed that the anti-insomnia effect was related to regulating the pathways of actin cytoskeleton and neuroactive ligand-receptor interactions. Transcriptomic and RT-qPCR analysis revealed that SFL significantly modulated the expression levels of neurod1, msh2, msh3, recql4, ercc5, rad5lc, and rev3l, which are mainly involved in neuron differentiation and DNA repair. Collectively, as a functional food, Fermented GE possessed potential for insomnia alleviation.

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Metabolomic navigated Citrus waste repurposing to restore amino acids disorder in neural lesion

Cited by 12 publications

References 40 publications

Cyclocodon lancifoliusfruit prolongs the lifespan ofCaenorhabditis elegans viaantioxidation and regulation of purine metabolism

Cyclocodon lancifoliusfruit prolongs the lifespan ofCaenorhabditis elegans viaantioxidation and regulation of purine metabolism

Insight into Lotusine and Puerarin in Repairing Alcohol-Induced Metabolic Disorder Based on UPLC-MS/MS

Sleep-promotion of probiotics-fermented Gastrodia elata Bl.: Chemical variation and omics exploration.

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