Metabolomic Profiling and Antioxidant Activity of Trigonella foenum-graecum and Trigonella hamosa

Abstract

Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 39 T HE METABOLOMIC profiling of Trigonella foenum-graecum and Trigonella hamosa seeds was performed using NMR spectroscopy combined with multivariate data analysis (MVDA). The database of the chemical shifts and the J-resolved two-dimensional NMR technique were used to identify the metabolites. Additionally, the identified metabolites were confirmed by comparison with previously identified compounds as well. Among the elucidated metabolites, choline, sterols, leucine, valine, flavonoids derivatives and trigonelline were found to contribute to distinguish between the two species of Trigonella in score scatter and loading plots of multivariate data analysis. Phytochemical analysis of plant seeds extracts for total carbohydrates, proteins, phenolics, flavonoids, saponins and anthocyanins was performed by spectrophotometer. The antioxidant potential of both species was evaluated by phosphomolybdenum method and DPPH radical scavenging activity. T. foenum-graecum was characterized by higher concentrations of trigonelline, sterols, protein, flavonoids derivatives, saponins and anthocyanins, and lower IC 50 in DPPH radical scavenging activity assay than T. hamosa. It was observed that total antioxidant capacity was positively correlated with glucose, trigonelline, protein, flavonoids, saponins and anthocyanins. T. foenum-graecum revealed an interesting metabolomic pattern with antioxidant activity that assigns this plant as a promising candidate to explore its targeted and non-targeted metabolomics profile along with potential bioactivities.

Genus Hypericum: General Properties, Chemical Contents and Biological Activities

“…Multiple sclerosis, Parkinson's illness, Alzheimer's disease, cardiac ailments, and other illnesses, neurodegenerative diseases, and cancer may arise as a result of oxidative stress (Selamoglu et al, 2020;Saridogan et al, 2021;Sevindik, 2021). Supplementary antioxidants are utilised in reducing the oxidative stress's consequences (Abdel-Farid et al, 2021;Eraslan et al, 2021;Abdel-alim et al, 2023). Plants are significant sources in terms of being a supplement for antioxidants.…”