Metabolomic Profiling and Neuroprotective Effects of Purslane Seeds Extract Against Acrylamide Toxicity in Rat’s Brain

Farag, Ola M.; Abd‐Elsalam, Reham M.; Ogaly, Hanan A.; Ali, Sara E.; Badawy, Shymaa A. El; Alsherbiny, Muhammad A.; Li, Chun Guang

doi:10.1007/s11064-020-03209-6

Cited by 43 publications

(34 citation statements)

References 103 publications

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“…Neuroprotective effect of seed and aqueous extract of Purslane in rats and mice was observed by several researchers (Hongxing et al, 2007;Abdel Moneim, 2013;Farag et al, 2021). Betacyanins from Purslane were found to be effective against the D-galactose induced neurotoxicity in mice (Wang et al, 2010).…”

Section: Neuroprotective Potentialmentioning

confidence: 95%

“…Nemzer et al (2020) identi ed widely known avonoids, such as quercetin, kaempferol, isorhamnetin and naringenin in Purslane. Several other phytoconstituents including 48 fatty acyl/lipids, 11 avonoids and its derivatives, seven carbohydrates, two glycosylated hydroxy-cinnamic acid derivatives and miscellaneous terpenoids, steroids, lignan and purine nucleosides were identi ed (Farag et al, 2021). Some of the very important metabolites found in Purslane and their bioactivities are described below.…”

Section: Phytochemical Richness Of Purslanementioning

confidence: 99%

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A review on bioactive phytochemicals, ethnomedicinal and pharmacological importance of Purslane (Portulaca oleracea L.)

Kumar

Sreedharan

Kashyap³

et al. 2021

Preprint

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The Portulaca oleracea L. commonly known as Purslane is distributed all over the world and easily grows in diverse soil conditions. It has been traditionally used as a nutritious and ethnomedicinal food across the globe. Various studies have shown that the plant is a rich source of various important phytochemicals such as flavonoids, alkaloids, terpenoids, proteins, carbohydrates, and vitamins such as A, C, E, and B, carotenoids and minerals such as phosphorus, calcium, magnesium and zinc. It is particularly very important because of the presence of very high concentration of omega-3- fatty acids especially α-linolenic acid, gamma- linolenic acid and linoleic acid, which are not generally synthesized in terrestrial plants. Various parts of Purslane are known for ethnomedicinal and pharmacological uses because of its anti-inflammatory, antidiabetic, skeletal muscle relaxant, antitumor, hepatoprotective, anticancer, antioxidant, anti-insomnia, analgesic, gastroprotective, neuroprotective, wound healing and antiseptic activities. Due to multiple benefits of Purslane, it has become an important wonder crop and various scientists across the globe have shown much interest in it as a healthy food for the future. In this review, we provide an update on the phytochemical and nutritional composition of Purslane, its usage as nutritional and an ethnomedicinal plant across the world. We further emphasize its ethno medicinal importance across cultures and pharmacological potential.

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Section: Neuroprotective Potentialmentioning

confidence: 95%

Section: Phytochemical Richness Of Purslanementioning

confidence: 99%

A review on bioactive phytochemicals, ethnomedicinal and pharmacological importance of Purslane (Portulaca oleracea L.)

Kumar

Sreedharan

Kashyap³

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Data were acquired in a profile mode and corrected with lock mass spray switching between the samples and external reference, allowing the MassLynx to ensure mass analysis accuracy continuously [212,213]. Leucine enkephaline (1 ng µL −1 ) was used as an external reference in 1:1 acetonitrile-water containing 0.1% formic acid at a flow rate of 5 µL/min via a LockSpray interface, generating a reference ion for negative ion mode [M-H] − of 554.261 m/z.…”

Section: Biochemometric and Lcms-driven Metabolomic Identification Of Anticancer Metabolites Of Ap-1 In The Mcf7 Human Breast Adenocarcinmentioning

confidence: 99%

Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells

Alsherbiny

Bhuyan

Radwan

et al. 2021

IJMS

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The combination of natural products with standard chemotherapeutic agents offers a promising strategy to enhance the efficacy or reduce the side effects of standard chemotherapy. Doxorubicin (DOX), a standard drug for breast cancer, has several disadvantages, including severe side effects and the development of drug resistance. Recently, we reported the potential bioactive markers of Australian propolis extract (AP−1) and their broad spectrum of pharmacological activities. In the present study, we explored the synergistic interactions between AP−1 and DOX in the MCF7 breast adenocarcinoma cells using different synergy quantitation models. Biochemometric and metabolomics-driven analysis was performed to identify the potential anticancer metabolites in AP−1. The molecular mechanisms of synergy were studied by analysing the apoptotic profile via flow cytometry, apoptotic proteome array and measuring the oxidative status of the MCF7 cells treated with the most synergistic combination. Furthermore, label-free quantification proteomics analysis was performed to decipher the underlying synergistic mechanisms. Five prenylated stilbenes were identified as the key metabolites in the most active AP−1 fraction. Strong synergy was observed when AP−1 was combined with DOX in the ratio of 100:0.29 (w/w) as validated by different synergy quantitation models implemented. AP−1 significantly enhanced the inhibitory effect of DOX against MCF7 cell proliferation in a dose-dependent manner with significant inhibition of the reactive oxygen species (p < 0.0001) compared to DOX alone. AP−1 enabled the reversal of DOX-mediated necrosis to programmed cell death, which may be advantageous to decline DOX-related side effects. AP−1 also significantly enhanced the apoptotic effect of DOX after 24 h of treatment with significant upregulation of catalase, HTRA2/Omi, FADD together with DR5 and DR4 TRAIL-mediated apoptosis (p < 0.05), contributing to the antiproliferative activity of AP−1. Significant upregulation of pro-apoptotic p27, PON2 and catalase with downregulated anti-apoptotic XIAP, HSP60 and HIF-1α, and increased antioxidant proteins (catalase and PON2) may be associated with the improved apoptosis and oxidative status of the synergistic combination-treated MCF7 cells compared to the mono treatments. Shotgun proteomics identified 21 significantly dysregulated proteins in the synergistic combination-treated cells versus the mono treatments. These proteins were involved in the TP53/ATM-regulated non-homologous end-joining pathway and double-strand breaks repairs, recruiting the overexpressed BRCA1 and suppressed RIF1 encoded proteins. The overexpression of UPF2 was noticed in the synergistic combination treatment, which could assist in overcoming doxorubicin resistance-associated long non-coding RNA and metastasis of the MCF7 cells. In conclusion, we identified the significant synergy and highlighted the key molecular pathways in the interaction between AP−1 and DOX in the MCF7 cells together with the AP−1 anticancer metabolites. Further in vivo and clinical studies are warranted on this synergistic combination.

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“…The supernatant was filtered through a 0.2 μm PTFE filter while the first 2 mL were discarded. The metabolomic analysis was performed in ACQUITY UPLC (Waters, USA) coupled with SYNAPT G2-S high-definition mass spectrometry (Waters Corp, Manchester, England) as reported in the literature [40]. Briefly, a 5 µL sample was injected at a flow rate of 400 µL/min.…”

Section: Uplc-ms Fingerprinting Of the Ar Extractmentioning

confidence: 99%

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect

et al. 2021

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Background Complementary remedies such as the Chinese herb ‘Sheng Ma’ (Black cohosh; Actaea racemosa ‘AR’) are being sought to overcome the shortcomings of conventional hormonal and surgical therapies developed for the treatment of polycystic ovary syndrome (PCOS). However, AR-induced hepatotoxicity necessitates a cautionary warning to be labeled on its products as recommended by the United States Pharmacopeia, where four out of seven hepatotoxic cases in Sweden were possibly associated with black cohosh products. Methods We investigated the effects, safety, and molecular targets of black cohosh ethanolic extract and/or vitamin C on ovarian functionality and oxidative response in hyperandrogenism-induced PCOS rats. A well-established rat model using oral letrozole, daily, for 21 days was employed. The rats then received the AR extract with and without vitamin C for 28 days. The hormonal evaluation, antioxidant status, histopathological examination, immunohistochemical analysis, cell proliferation, and the expression ratio of the aromatase (Cyp19α1) gene were evaluated. Additionally, holistic profiling of the AR arsenal of secondary metabolites was performed using ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole high-resolution time of flight mass spectrometry (QTOF-MS). Results Beneficial effects were exerted by AR in PCOS rats as antioxidant status, hormonal profile, lipid profile, glucose level, liver functions, and the induced Ki-67 expression in the granulosa, theca cell layers and interstitial stromal cells were all improved. Notably, the combination of AR with vitamin C was not only more effective in reversing the dysregulated levels of testosterone, luteinizing hormone, and mRNA level of Cyp19α1 gene in the PCOS rat, but also safer. The combination regulated both ovarian and hepatic malondialdehyde (MDA) and glutathione (GSH) levels with histological improvement observed in the liver and ovaries. In addition, the untargeted metabolomic profiling enabled the identification of 61 metabolites allocated in five major chemical classes. Conclusion This study demonstrated the benefit of the combinatorial effects of AR and vitamin C in mitigating the reproductive and metabolic disorders associated with PCOS with the elimination of AR hepatotoxic risk.

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Metabolomic Profiling and Neuroprotective Effects of Purslane Seeds Extract Against Acrylamide Toxicity in Rat’s Brain

Cited by 43 publications

References 103 publications

A review on bioactive phytochemicals, ethnomedicinal and pharmacological importance of Purslane (Portulaca oleracea L.)

A review on bioactive phytochemicals, ethnomedicinal and pharmacological importance of Purslane (Portulaca oleracea L.)

Metabolomic Identification of Anticancer Metabolites of Australian Propolis and Proteomic Elucidation of Its Synergistic Mechanisms with Doxorubicin in the MCF7 Cells

Modulation of steroidogenesis by Actaea racemosa and vitamin C combination, in letrozole induced polycystic ovarian syndrome rat model: promising activity without the risk of hepatic adverse effect

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