Metabolomic Strategies Based on High-Resolution Mass Spectrometry as a Tool for Recognition of GMO (MON 89788 Variety) and Non-GMO Soybean: a Critical Assessment of Two Complementary Methods

Hrbek, Vojtěch; Krtkova, Veronika; Rubert, Josep; Chmelařová, Hana; Demnerova, Kateřina; Ovesná, Jaroslava; Hajšlová, Jana

doi:10.1007/s12161-017-0929-8

Cited by 14 publications

(4 citation statements)

References 34 publications

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“…Other than this, metabolomic studies are opted for ensuring the safety levels of GM crops and used effectively to analyse the differential fluctuation of metabolites (Kusano et al 2011, 2015, Rao et al 2016, Hrbek et al 2017, Corujo et al 2019). Indeed, it is expected to be challenging to execute safety evaluations with previous strategies for transgenic crops whose genetic diversity matches that of conventional crops.…”

Section: Application Of Metabolomics In Crop Improvementmentioning

confidence: 99%

Metabolomics‐driven investigation of plant defense response against pest and pathogen attack

Kumari,

Yagnik,

Gupta

et al. 2024

Physiologia Plantarum

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The advancement of metabolomics has assisted in the identification of various bewildering characteristics of the biological system. Metabolomics is a standard approach, facilitating crucial aspects of system biology with absolute quantification of metabolites using minimum samples, based on liquid/gas chromatography, mass spectrometry and nuclear magnetic resonance. The metabolome profiling has narrowed the wide gaps of missing information and has enhanced the understanding of a wide spectrum of plant–environment interactions by highlighting the complex pathways regulating biochemical reactions and cellular physiology under a particular set of conditions. This high throughput technique also plays a prominent role in combined analyses of plant metabolomics and other omics datasets. Plant metabolomics has opened a wide paradigm of opportunities for developing stress‐tolerant plants, ensuring better food quality and quantity. However, despite advantageous methods and databases, the technique has a few limitations, such as ineffective 3D capturing of metabolites, low comprehensiveness, and lack of cell‐based sampling. In the future, an expansion of plant–pathogen and plant–pest response towards the metabolite architecture is necessary to understand the intricacies of plant defence against invaders, elucidation of metabolic pathway operational during defence and developing a direct correlation between metabolites and biotic stresses. Our aim is to provide an overview of metabolomics and its utilities for the identification of biomarkers or key metabolites associated with biotic stress, devising improved diagnostic methods to efficiently assess pest and pathogen attack and generating improved crop varieties with the help of combined application of analytical and molecular tools.

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Section: Application Of Metabolomics In Crop Improvementmentioning

confidence: 99%

Metabolomics‐driven investigation of plant defense response against pest and pathogen attack

Kumari,

Yagnik,

Gupta

et al. 2024

Physiologia Plantarum

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“…Metabolomics-guided plant breeding programs such as mGWAS (Metabolite Genome-Wide Association Study) and mQTL (methylation quantitative trait locus) mGWAS analysis has proven to be critical for dissecting the genetic and metabolic architecture of rice by finding the genes related with natural variation in rice metabolism [ 7 , 90 , 98 , 99 ]. Chen and colleagues (2014) used GWAS to detect 6.4 million SNPs from 529 distinct rice strains, and 36 potential genes that regulate the levels of at least 34 recognized primary and secondary metabolites were identified.…”

Section: Application Of Metabolomics As a Prospective Tool To Improve...mentioning

confidence: 99%

Metabolomics as a Prospective Tool for Soybean (Glycine max) Crop Improvement

Ncube

Mohale

Nogemane

2022

CIMB

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Global demand for soybean and its products has stimulated research into the production of novel genotypes with higher yields, greater drought and disease tolerance, and shorter growth times. Genetic research may be the most effective way to continue developing high-performing cultivars with desirable agronomic features and improved nutritional content and seed performance. Metabolomics, which predicts the metabolic marker for plant performance under stressful conditions, is rapidly gaining interest in plant breeding and has emerged as a powerful tool for driving crop improvement. The development of increasingly sensitive, automated, and high-throughput analytical technologies, paired with improved bioinformatics and other omics techniques, has paved the way for wide characterization of genetic characteristics for crop improvement. The combination of chromatography (liquid and gas-based) with mass spectrometry has also proven to be an indisputable efficient platform for metabolomic studies, notably plant metabolic fingerprinting investigations. Nevertheless, there has been significant progress in the use of nuclear magnetic resonance (NMR), capillary electrophoresis, and Fourier-transform infrared spectroscopy (FTIR), each with its own set of benefits and drawbacks. Furthermore, utilizing multivariate analysis, principal components analysis (PCA), discriminant analysis, and projection to latent structures (PLS), it is possible to identify and differentiate various groups. The researched soybean varieties may be correctly classified by using the PCA and PLS multivariate analyses. As metabolomics is an effective method for evaluating and selecting wild specimens with desirable features for the breeding of improved new cultivars, plant breeders can benefit from the identification of metabolite biomarkers and key metabolic pathways to develop new genotypes with value-added features.

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“…Plant metabolomics has been adapted for the safety assessment of GM crops and derived foods [ 24 , 26 , 136 , 137 , 138 ], albeit that its usefulness is still a hot debate [ 25 , 139 ]. For this purpose, comparative metabolomic analysis between a GM and its non-GM counterpart is performed, and the resulting results usually form the basis for substantial equivalence assessment.…”

Section: The Applications Of Plant Metabolomics In Crop Improvemenmentioning

confidence: 99%

“…Therefore, metabolomes revealed by any combined metabolomics methods reflect only the tip of the iceberg of a cell, tissue, or organism. Nevertheless, the power (accuracy and coverage of metabolite measurements) of current metabolomics and bioinformatics is strong enough to support its application in both basic and applied research, which significantly facilitates crop breeding for yield and quality [ 15 , 17 , 18 , 19 , 20 ], plant-derived drug discovery and production [ 21 , 22 , 23 ], safety assessment of genetically modified (GM) crops [ 24 , 25 , 26 ], and biohazards derived from plants [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Promoting Human Nutrition and Health through Plant Metabolomics: Current Status and Challenges

Sun

Chen

Hong

et al. 2020

Biology

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Plant metabolomics plays important roles in both basic and applied studies regarding all aspects of plant development and stress responses. With the improvement of living standards, people need high quality and safe food supplies. Thus, understanding the pathways involved in the biosynthesis of nutritionally and healthily associated metabolites in plants and the responses to plant-derived biohazards in humans is of equal importance to meet people’s needs. For each, metabolomics has a vital role to play, which is discussed in detail in this review. In addition, the core elements of plant metabolomics are highlighted, researches on metabolomics-based crop improvement for nutrition and safety are summarized, metabolomics studies on plant natural products including traditional Chinese medicine (TCM) for health promotion are briefly presented. Challenges are discussed and future perspectives of metabolomics as one of the most important tools to promote human nutrition and health are proposed.

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Metabolomic Strategies Based on High-Resolution Mass Spectrometry as a Tool for Recognition of GMO (MON 89788 Variety) and Non-GMO Soybean: a Critical Assessment of Two Complementary Methods

Cited by 14 publications

References 34 publications

Metabolomics‐driven investigation of plant defense response against pest and pathogen attack

Metabolomics‐driven investigation of plant defense response against pest and pathogen attack

Metabolomics as a Prospective Tool for Soybean (Glycine max) Crop Improvement

Promoting Human Nutrition and Health through Plant Metabolomics: Current Status and Challenges

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