Metabolomics: A promising technique for uncovering quality-attribute of fresh and processed fruits and vegetables

Wang, Kewen; Liao, Xiaojun; Xia, Jianguo; Xiao, Cheng; Deng, Jianjun; Xu, Zhenzhen

doi:10.1016/j.tifs.2023.104213

Cited by 6 publications

(4 citation statements)

References 90 publications

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“…Although NMR metabolite profiling is currently considered a reliable approach for the assessment of presence and relative abundance of small molecules [27,29,81,82], genetic information is, at present, the only method that provides a final validation with respect to the description of plant ecotypes [83][84][85][86]. Further studies will be needed to explore genetic information, thus providing a more comprehensive characterization of the Vessalico ecotype.…”

Section: Discussionmentioning

confidence: 99%

NMR Metabolite Profiling for the Characterization of Vessalico Garlic Ecotype and Bioactivity against Xanthomonas campestris pv. campestris

Iobbi,

Parisi,

Lanteri

et al. 2024

Plants

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The Italian garlic ecotype “Vessalico” possesses distinct characteristics compared to its French parent cultivars Messidor and Messidrôme, used for sowing, as well as other ecotypes in neighboring regions. However, due to the lack of a standardized seed supply method and cultivation protocol among farmers in the Vessalico area, a need to identify garlic products that align with the Vessalico ecotype arises. In this study, an NMR-based approach followed by multivariate analysis to analyze the chemical composition of Vessalico garlic sourced from 17 different farms, along with its two French parent cultivars, was employed. Self-organizing maps allowed to identify a homogeneous subset of representative samples of the Vessalico ecotype. Through the OPLS-DA model, the most discriminant metabolites based on values of VIP (Variable Influence on Projections) were selected. Among them, S-allylcysteine emerged as a potential marker for distinguishing the Vessalico garlic from the French parent cultivars by NMR screening. Additionally, to promote sustainable agricultural practices, the potential of Vessalico garlic extracts and its main components as agrochemicals against Xanthomonas campestris pv. campestris, responsible for black rot disease, was explored. The crude extract exhibited a MIC of 125 μg/mL, and allicin demonstrated the highest activity among the tested compounds (MIC value of 31.25 μg/mL).

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Section: Discussionmentioning

confidence: 99%

NMR Metabolite Profiling for the Characterization of Vessalico Garlic Ecotype and Bioactivity against Xanthomonas campestris pv. campestris

Iobbi,

Parisi,

Lanteri

et al. 2024

Plants

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show abstract

“…Most nutritional studies have been limited to analyses of target metabolites, such as carbohydrates, amino acids, and organic acids, which are mainly relevant to taste, or certain metabolites with known bioactive functions [15][16][17]. Recently, advanced highresolution mass spectrometry (e.g., gas chromatography-mass spectrometry and ultraperformance liquid chromatography-tandem mass spectrometry) and metabolomic approaches have been used to widen the understanding of metabolite composition in foods and plants through untargeted metabolomics [18][19][20]. This helps to analyze a variety of small molecules (≤1500 Da), including primary and secondary metabolites, relevant to the taste, aroma, color, and nutritional qualities of food [21].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Reveals the Effects of Nitrogen/Phosphorus/Potassium (NPK) Fertilizer Levels on Cucumber Fruit Raised in Different Nutrient Soils

Lee,

Kim,

Lee

et al. 2024

Metabolites

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Fertilizers are widely used to improve the quality of fruits and vegetables. However, the overuse of fertilizers has become an issue because it causes environmental problems and negatively affects productivity and fruit quality. In this study, we examined the effects of nitrogen, phosphorus, and potassium (NPK) fertilizer levels on the metabolism of cucumber fruit in low- and high-nutrient soils using mass-spectrometry-based metabolomics approaches. Cucumber metabolite content was notably different depending on the initial soil nutrient status. Most amino acids and phenylpropanoids were abundant in the cucumbers raised in low-nutrient soil, whereas organic acids, some amino acids (aspartate, glutamate, and ornithine), and carbohydrates were comparatively higher in fruits from high-nutrient soil. The fertilizer supply resulted in an alteration in the metabolite profile, while no change in fruit yield was observed in either low- or high-nutrient soils. Fertilizer treatment perturbed the metabolite contents in cucumbers from low-nutrient soil. In contrast, treatment with higher concentrations of fertilizer in high-nutrient soil increased phenylpropanoid content in the cucumbers, while most metabolites decreased. In conclusion, fertilization levels should be carefully determined, considering culture conditions such as the original soil status, to increase product yield and fruit quality and avoid environmental problems.

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“…For example, metagenomic analysis reveals that differences in amino acid and carbohydrate metabolism pathways between sorghum substrates alter the volatility of Chinese strong-flavored Baijiu [9] . Similarly, within fermented vegetables, the substrate of garlic influences the microbial ecology and metabolite profile of kimchi [10] , and the selection of chili pepper had a significant effect on the physiochemical property and the flavor of Paojiao, a fermented Chinese chili pepper [11] . However, there is a lack of knowledge regarding the effects of different substrates/supplier patterns on non-salt Suancai.…”

Section: Introductionmentioning

confidence: 99%

“…Metabolomics involves comprehensive identification and quantification of small molecules (≤ 1,500 Da). When typically coupled with nuclear magnetic resonance (NMR) or mass spectrometry (MS) with upstream separation methods, it can improve the understanding of the quality attribute of foods at the molecular level [ 12 ] . For example, metabolomic analysis revealed both targeted and untargeted metabolites of fermented soybean foods, which suggested their role in flavor development and therapeutic properties of them [ 13 ] .…”

Section: Introductionmentioning

confidence: 99%

Effects of substrates and suppliers of ingredients on microbial community and metabolites of traditional non-salt Suancai

Lai,

Yan,

Wang

et al. 2024

Microbiome Res Rep

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Aim: Non-salt Suancai is an acidic fermented vegetable consumed by the Chinese Yi ethnic group. Traditionally, it is produced by fermentation without salt in a cold environment. The present study aimed to investigate the metabolite and microbial characteristics, and the effects of substrates/suppliers ingredients on non-salt Suancai. Methods: A simulated fermentation system of non-salt Suancai was constructed by using different substrates/suppliers’ ingredients. The coherence and differential detection of the metabolite and microbial characteristics were done through non-target metabolomic and metagenomic analysis. Results: Lactic acid was the predominant organic acid across all samples. The enumeration of the Lactic acid bacteria showed no discernible differences between study groups, but that of yeast was highest in the mustard leaf stem (Brassica juncea var. latipa ). The three major biological metabolic pathways were metabolism, environmental information, and genetic information processing based on the KEGG database. The metabolite diversity varied with the substrate/supplier of ingredients based on the PLS-DA plot. Lactiplantibacillus , Leuconostoc , and Lactococcus were prevalent in all samples but differentially. The microbial diversity and richness varied significantly, with 36~291 species being identified. Among the various substrates collected from the same supplier, 29, 59, and 29 differential species were identified based on LEfSe [linear discriminant analysis (LDA) > 2, P < 0.05]. Leuconostoc citreum , Leuconostoc mesenteroides , Leuconostoc pseudomesenteroides , Lactiplantibacillus plantarum , and Leuconostoc lactis were likely to be used as the species to discriminate samples collected from different suppliers. Conclusions: This research contributed to the exploration of microbial and metabolite characteristics behind the ingredient restriction of non-salt Suancai using traditional technology.

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Metabolomics: A promising technique for uncovering quality-attribute of fresh and processed fruits and vegetables

Cited by 6 publications

References 90 publications

NMR Metabolite Profiling for the Characterization of Vessalico Garlic Ecotype and Bioactivity against Xanthomonas campestris pv. campestris

NMR Metabolite Profiling for the Characterization of Vessalico Garlic Ecotype and Bioactivity against Xanthomonas campestris pv. campestris

Metabolomics Reveals the Effects of Nitrogen/Phosphorus/Potassium (NPK) Fertilizer Levels on Cucumber Fruit Raised in Different Nutrient Soils

Effects of substrates and suppliers of ingredients on microbial community and metabolites of traditional non-salt Suancai

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