Metabolomics and physiological analyses reveal β-sitosterol as an important plant growth regulator inducing tolerance to water stress in white clover

Li, Zhou; Cheng, Bizhen; Yong, Bin; Liu, Ting; Peng, Yan; Zhang, Xinquan; Ma, Xiao; Huang, Linkai; Liu, Wei; Nie, Gang

doi:10.1007/s00425-019-03277-1

Cited by 41 publications

(33 citation statements)

References 52 publications

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“…In addition, glyceric acid, a component of glycolysis, was associated with a greater ability to absorb nutrients and water of wheat under drought conditions [4]. The founding of Li et al indicated that exogenous β-sitosterol promoted the accumulation of glyceric acid contributing to improved drought tolerance in white clover [56]. These results implied that COR-mediated tolerance to osmotic stress is associated with organic acids belonging to both intermediates and non-intermediates involved in the TCA cycle.…”

Section: Exogenous Cor Enhances the Accumulation Of Organic Acids Undmentioning

confidence: 97%

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Zhou

et al. 2020

Biomolecules

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Drought is a major abiotic stress that restricts plants growth, development, and yield. Coronatine (COR), a mimic of JA-Ile, functions in plant tolerance to multiple stresses. In our study, we examined the effects of COR in tobacco under polyethylene glycol (PEG) stress. COR treatment improved plant growth under stress as measured by fresh weight (FW) and dry weight (DW). The enzyme activity assay indicated that, under osmotic stress conditions, the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were enhanced by COR treatment. Histochemical analyses via nitrotetrazolium blue chloride (NBT) and 3,3′-diaminobenzidine (DAB) staining showed that COR reduced reactive oxygen species (ROS) accumulation during osmotic stress. Metabolite profiles revealed that COR triggered significant metabolic changes in tobacco leaves under osmotic stress, and many essential metabolites, such as sugar and sugar derivatives, organic acids, and nitrogen-containing compounds, which might play active roles in osmotic-stressed tobacco plants, were markedly accumulated in the COR-treated tobacco. The work presented here provides a comprehensive understanding of the COR-mediated physiological, biochemical, and metabolic adjustments that minimize the adverse impact of osmotic stress on tobacco.

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Section: Exogenous Cor Enhances the Accumulation Of Organic Acids Undmentioning

confidence: 97%

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Zhou

et al. 2020

Biomolecules

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“…Therefore, plant metabolism is a dynamic process that responds to external stimuli. Environmental changes such as light quality, water stress, or temperature have been shown to impact metabolism [8][9][10][11][12][13]. This review focuses on the roles of primary and specialized metabolism in defense responses or disease progression during plant-pathogen interactions.…”

Section: The Evolution Of Metabolomics In the Study Of Plant-microbe mentioning

confidence: 99%

Metabolomics as an Emerging Tool for the Study of Plant–Pathogen Interactions

et al. 2020

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Plants defend themselves from most microbial attacks via mechanisms including cell wall fortification, production of antimicrobial compounds, and generation of reactive oxygen species. Successful pathogens overcome these host defenses, as well as obtain nutrients from the host. Perturbations of plant metabolism play a central role in determining the outcome of attempted infections. Metabolomic analyses, for example between healthy, newly infected and diseased or resistant plants, have the potential to reveal perturbations to signaling or output pathways with key roles in determining the outcome of a plant–microbe interaction. However, application of this -omic and its tools in plant pathology studies is lagging relative to genomic and transcriptomic methods. Thus, it is imperative to bring the power of metabolomics to bear on the study of plant resistance/susceptibility. This review discusses metabolomics studies that link changes in primary or specialized metabolism to the defense responses of plants against bacterial, fungal, nematode, and viral pathogens. Also examined are cases where metabolomics unveils virulence mechanisms used by pathogens. Finally, how integrating metabolomics with other -omics can advance plant pathology research is discussed.

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“…Sterols, in addition to regulating fluidity and permeability of the membranes, have shown capacity to intercept free radicals. Recently, Li et al (2019) reported that application of β ‐sitosterol could improve drought tolerance of white clover enhancing the accumulation of metabolites associated with growth maintenance, osmotic adjustment and antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide and abscisic acid regulate osmoprotective and antioxidative mechanisms related to water stress tolerance of grapevines

Pontín

Murcia

Fontana

et al. 2021

Australian Journal of Grape and Wine Research

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Background and Aims Exogenously applied nitric oxide (NO) and abscisic acid (ABA) are known to improve the tolerance of plants to abiotic stress. The effects of NO and ABA applications on physiological and metabolic responses associated with vine protection against water stress were analysed. Methods and Results The responses to the NO donor sodium nitroprusside (hereinafter referred to as NO treatment) and ABA were assessed on metabolic profiling, the activity of antioxidant enzymes, and physiological parameters in leaves of water‐stressed Malbec vines. Application of NO and ABA partially closed stomata, thus increasing water potential and reducing vine growth. As well, NO and ABA increased guaiacol peroxidase activity and the concentration of specific sugars and anthocyanins in leaves, whereas the accumulation of amino acids was reduced, in turn associated with less protein degradation. Differential responses triggered by NO in stimulating ascorbate peroxidase activity and in incrementing tricarboxylic acid cycle intermediates and terpenes that stabilise membranes, suggest differential mechanisms for NO and ABA in counteracting water stress effects in leaves of grapevines. Conclusions Nitric oxide and ABA differentially regulate osmoprotective and antioxidative mechanisms related to water stress tolerance in grapevines. Significance of the Study Spray application of NO and ABA to whole plants promoted leaf metabolic responses known to be associated with water stress tolerance mechanisms in grapevines. This approach may provide the basis for improving viticulture in grapegrowing regions affected by water scarcity due to climate change.

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Metabolomics and physiological analyses reveal β-sitosterol as an important plant growth regulator inducing tolerance to water stress in white clover

Cited by 41 publications

References 52 publications

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Combining Physiological and Metabolomic Analysis to Unravel the Regulations of Coronatine Alleviating Water Stress in Tobacco (Nicotiana tabacum L.)

Metabolomics as an Emerging Tool for the Study of Plant–Pathogen Interactions

Nitric oxide and abscisic acid regulate osmoprotective and antioxidative mechanisms related to water stress tolerance of grapevines

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