Metabolomics and preterm birth: What biomarkers in cervicovaginal secretions are predictive of high-risk pregnant women?

Auray‐Blais, Christiane; Raiche, Evelyne; Gagnon, René; Berthiaume, Maryse; Pasquier, Jean-Charles

doi:10.1016/j.ijms.2011.02.009

Cited by 26 publications

(23 citation statements)

References 12 publications

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“…30,31 Only one other study has examined the metabolome in CV fluid. 32 While these authors found that in the setting of a short cervix, the CV metabolome distinguished women with term and preterm birth, they did not report the distinct metabolic pathways that differed between the two groups. 32 Our study is unique in that, using a metabolomics approach, it provides the first comprehensive, detailed description of metabolites that are present in the cervico-vaginal space that were distinct in women who ultimately had a PTB.…”

Section: Resultsmentioning

confidence: 98%

“…32 While these authors found that in the setting of a short cervix, the CV metabolome distinguished women with term and preterm birth, they did not report the distinct metabolic pathways that differed between the two groups. 32 Our study is unique in that, using a metabolomics approach, it provides the first comprehensive, detailed description of metabolites that are present in the cervico-vaginal space that were distinct in women who ultimately had a PTB. While we would not expect the metabolome in amniotic fluid to mirror the CV space, it is notable that these studies found a metabolomic profile that was associated with inflammation.…”

Section: Resultsmentioning

confidence: 98%

“…32 Both studies of amniotic fluid demonstrated alterations in xenobiotic metabolism among women with PTB. 30,31 Only one other study has examined the metabolome in CV fluid.…”

Section: Resultsmentioning

confidence: 99%

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Women with preterm birth have a distinct cervicovaginal metabolome

Ghartey

Bastek

Brown

et al. 2015

American Journal of Obstetrics and Gynecology

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Objective Metabolomics has the potential to reveal novel pathways involved in the pathogenesis of preterm birth (PTB). The objective of this study was to investigate if the cervico-vaginal (CV) metabolome was different in asymptomatic women destined to have a PTB compared to term birth. Study Design A nested case-control study was performed using CV fluid collected from a larger prospective cohort. CV fluid was collected between 20-24 weeks (V1) and 24-28 weeks (V2). The metabolome was compared between women with a spontaneous PTB (n=10) to women who delivered at term (n=10). Samples were extracted and prepared for analysis using a standard extraction solvent method (Metabolon®, Durham, NC). Global biochemical profiles were determined using GC/MS and UPLC MS/MS. ANOVA was used to detect differences in biochemical compounds between the groups. A false discovery rate was estimated to account for multiple comparisons. Result A total of 313 biochemicals were identified in CV fluid. 82 biochemicals were different in the CV fluid at V1 in those destined to have a PTB compared to term birth while 48 were different at V2 (Table 1). Amino acid, carbohydrate, and peptide metabolites were distinct between women with and without PTB. Conclusion These data suggest that the CV space is metabolically active during pregnancy. Changes in the CV metabolome may be observed weeks, if not months, prior to any clinical symptoms. Understanding the CV metabolome may hold promise for unraveling the pathogenesis of PTB and may provide novel biomarkers to identify women most at risk.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 98%

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Women with preterm birth have a distinct cervicovaginal metabolome

Ghartey

Bastek

Brown

et al. 2015

American Journal of Obstetrics and Gynecology

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“…Noninvasive collection of cervicovaginal secretions from 15 women (women with a short cervix who had a spontaneous preterm birth compared to a control cohort with a short cervix and with a long cervix) was performed, and 17 markers of interest were identified. This particular metabolomic approach may help in the management of women at highrisk for spontaneous preterm birth [16]. …”

Section: Metabolomics In Vaginal Secretionmentioning

confidence: 99%

Metabolomics Application in Maternal-Fetal Medicine

Fanos

Atzori

Makarenko

et al. 2013

BioMed Research International

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Metabolomics in maternal-fetal medicine is still an “embryonic” science. However, there is already an increasing interest in metabolome of normal and complicated pregnancies, and neonatal outcomes. Tissues used for metabolomics interrogations of pregnant women, fetuses and newborns are amniotic fluid, blood, plasma, cord blood, placenta, urine, and vaginal secretions. All published papers highlight the strong correlation between biomarkers found in these tissues and fetal malformations, preterm delivery, premature rupture of membranes, gestational diabetes mellitus, preeclampsia, neonatal asphyxia, and hypoxic-ischemic encephalopathy. The aim of this review is to summarize and comment on original data available in relevant published works in order to emphasize the clinical potential of metabolomics in obstetrics in the immediate future.

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“…Vaginal swab sampling [19,33,34,149,157,158] or the collection of cervicovaginal lavage (CVL) [125,156,159] represent the two most common sampling techniques for metabolic profiling studies of the vaginal mucosa. To ensure adequate amount of vaginal mucosa is collected, the swab is rolled in a clockwise direction and moved across all four quadrants of the posterior fornix.…”

Section: Sampling and Metabolite Extractionmentioning

confidence: 99%

Assessment of microbiota:host interactions at the vaginal mucosa interface

Pruski

Lewis

Lee

et al. 2018

Methods

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There is increasing appreciation of the role that vaginal microbiota play in health and disease throughout a woman's lifespan. This has been driven partly by molecular techniques that enable detailed identification and characterisation of microbial community structures. However, these methods do not enable assessment of the biochemical and immunological interactions between host and vaginal microbiota involved in pathophysiology. This review examines our current knowledge of the relationships that exist between vaginal microbiota and the host at the level of the vaginal mucosal interface. We also consider methodological approaches to microbiomic, immunologic and metabolic profiling that permit assessment of these interactions. Integration of information derived from these platforms brings the potential for biomarker discovery, disease risk stratification and improved understanding of the mechanisms regulating vaginal microbial community dynamics in health and disease.

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Metabolomics and preterm birth: What biomarkers in cervicovaginal secretions are predictive of high-risk pregnant women?

Cited by 26 publications

References 12 publications

Women with preterm birth have a distinct cervicovaginal metabolome

Women with preterm birth have a distinct cervicovaginal metabolome

Metabolomics Application in Maternal-Fetal Medicine

Assessment of microbiota:host interactions at the vaginal mucosa interface

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