Metabolomics and Proteomics Reveal the Variation of Substances in Apheresis Platelets during Storage and Their Effects on Cancer Cell Proliferation

Abstract

<b><i>Introduction:</i></b> Apheresis platelets (APs) are clinically and crucially important in the prevention and treatment of bleeding in patients with thrombocytopenia or cancer. However, few researchers have addressed the variation of supernatant metabolites and exosome proteins in APs during storage and their effects on cancer cell proliferation. <b><i>Objective:</i></b> This study was designed to explore the change rules of the metabolites and exosomal proteins of APs during storage and their effects on cancer cell proliferation. <b><i>Methods:</i></b> Metabolomics and proteomics were separately applied to analyze the variation of AP supernatant metabolites and exosomal proteins between freshly prepared day-0 and day-5 terminal-stored APs. Cell counting kit (CCK)-8 assay was performed to detect the effects of AP supernatants and exosomes on the proliferation of cancer cells. <b><i>Results:</i></b> We found that the supernatant metabolites and exosomal proteins in APs were significantly different on day 0 and day 5, and that many differential metabolites and exosomal proteins were associated with cancer characteristics. Furthermore, the day-5 AP supernatants had a greater inhibition of the proliferation of K562, HepG2, and HCT116 cancer cells, but the day-5 AP exosomes had no significant effect on the proliferation of these cancer cells. <b><i>Conclusion:</i></b> The variant terminal-stored AP supernatants may inhibit the proliferation of cancer cells but the variant terminal AP exosomes have no effect on cancer cell proliferation.

Platelet membrane‐derived microparticles may be biomarkers in patients with hepatocellular carcinoma and can promote the invasion and metastasis of hepatoma carcinoma cells

“…37,38 Our previous experiments confirmed that the exosomes of platelets have no obvious effect on tumor proliferation, while the supernatant has an inhibitory effect on tumor cells, which also shows the dual effect of preserved platelets on tumors. 14,15 Contrary to previous reports, our study showed that PMPs may act in different ways on different tumor cells, PMPs promoted the invasion and metastasis of HCC cells, and hardly caused apoptosis, which also ensured the escape of HCC cells.…”

“…[11][12][13] In our previous study, we found that substances released during APs preservation were involved in the process of cancer cells. 14,15 Therefore, we focused on the study of the changes in the content of a small fraction of microparticles released by APs in the Chinese population. The study of microparticles released during platelet preservation has an important reference basis for improving platelet preparation, storage conditions and transportation, and has important clinical significance for guiding the clinical transfusion of APs.…”

“…PMPs are an important factor in transfusion medicine, and high PMPs in the transfused platelet product could reduce platelet recovery by a direct effect on the recipient's immune system 11–13 . In our previous study, we found that substances released during APs preservation were involved in the process of cancer cells 14,15 . Therefore, we focused on the study of the changes in the content of a small fraction of microparticles released by APs in the Chinese population.…”

Changes in inflammatory responses and autophagy during apheresis platelet preservation and its correlation with platelet transfusion refractoriness in patients with acute lymphoblastic leukemia

“…Apheresis platelet (AP) transfusion, as a potentially life-saving treatment, is commonly employed to address bleeding complications associated with thrombocytopenia [ 6 ]. APs, obtained from a single donor, possess high platelet concentration, purity, and allow precise control of the infusion dose [ 7 , 8 ].…”

A pilot study of the metabolic profiles of apheresis platelets modified by donor age and sex and in vitro short‐term incubation with sex hormones

“…2 A number of metabolomic studies have been published using apheresis platelets and have characterized the metabolomic profiles of both the plasma fraction of apheresis platelets and the metabolomic profiles of the platelets themselves and have looked at the differences imparted by routine storage, cold storage, additive solutions, and donor sex. [10][11][12][13][14][15][16] Apheresis platelets are biologically relevant and represent a life-saving therapy for those patients requiring extended surgeries, organ transplantation, myelotoxic chemotherapy, and bone marrow transplantation and the severely injured. Previous studies have shown a significant impact of apheresis versus buffy coat platelet collection procedures on the metabolome of platelets for transfusion purposes.…”