Metabolomics: Application in Plant Sciences

Glauser, Gaëtan; Bernard, Julien; Wolfender, Jean‐Luc

doi:10.1002/9783527655861.ch13

Cited by 9 publications

(6 citation statements)

References 160 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, absolute quantification of signals is not possible in the absence of reference standards because ionization is compound-dependent. Finally, in comparison to NMR, the relatively poor reproducibility of MS may render its use in long-term studies problematic when samples cannot be stored for a prolonged period of time (Glauser et al, 2013). …”

Section: Current Metabolomic Technologiesmentioning

confidence: 99%

“…The number of publications which report the use of UHPLC-MS for metabolomic studies has grown exponentially over the last years and the trend will definitely not be reversed in the near future. Still, whatever powerful they are, LC-MS and UHPLC-MS cannot replace all other techniques because they also present some limitations, such as the problem of ion suppression and the lack of reproducibility of fragmentation spectra which complicates the creation of mass spectral libraries based on LC-MS data (Glauser et al, 2013). …”

Section: Current Metabolomic Technologiesmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomics of cereals under biotic stress: current knowledge and techniques

Balmer¹,

Flors²,

Glauser³

et al. 2013

Front. Plant Sci.

128

108

View full text Add to dashboard Cite

Prone to attacks by pathogens and pests, plants employ intricate chemical defense mechanisms consisting of metabolic adaptations. However, many plant attackers are manipulating the host metabolism to counteract defense responses and to induce favorable nutritional conditions. Advances in analytical chemistry have allowed the generation of extensive metabolic profiles during plant-pathogen and pest interactions. Thereby, metabolic processes were found to be highly specific for given tissues, species, and plant-pathogen/pest interactions. The clusters of identified compounds not only serve as base in the quest of novel defense compounds, but also as markers for the characterization of the plants' defensive state. The latter is especially useful in agronomic applications where meaningful markers are essential for crop protection. Cereals such as maize make use of their metabolic arsenal during both local and systemic defense responses, and the chemical response is highly adapted to specific attackers. Here, we summarize highlights and recent findings of metabolic patterns of cereals under pathogen and pest attack.

show abstract

Section: Current Metabolomic Technologiesmentioning

confidence: 99%

Section: Current Metabolomic Technologiesmentioning

confidence: 99%

Metabolomics of cereals under biotic stress: current knowledge and techniques

Balmer¹,

Flors²,

Glauser³

et al. 2013

Front. Plant Sci.

128

108

View full text Add to dashboard Cite

show abstract

“…However, the integration of all the data obtained during such an approach into a single statistical model remains challenging. Although appropriate statistical methods to merge multiple datasets are available (Forshed et al, 2007;Glauser et al, 2013a), experiments that combine more than one analytical technology remain an exception, mostly because they are laborious and expensive. In the future, the application of metabolomics is likely to become much more user-friendly in a way that a researcher may spend less time on methodological details thanks to simplified workflows.…”

Section: Discussionmentioning

confidence: 99%

“…4 Depends on the analyzer: single quadrupole MS are pretty cheap whereas TOF-MS are quite expensive. Table 1 Current advantages and limitations of analytical technologies used in metabolomics (adapted in part from Wolfender et al, 2013) Unfortunately, in LC-MS fragmentation is instrumentand condition-dependent, which makes the creation of spectral databases and the comparison between experimental and library spectra complicated (Glauser et al, 2013a). Consequently, alternatives must be found including, for instance, the determination of molecular formulae as a starting point for metabolite identification combined with targeted collision-induced dissociation (CID)-MS/MS experiments and in-silico fragmentation prediction tools (Wolf et al, 2010;Zhou et al, 2014) or mass spectral networking (Rasche et al, 2012;Watrous et al, 2012).…”

mentioning

confidence: 99%

Metabolomics in plant–herbivore interactions: challenges and applications

Maag

Erb

Glauser

2015

Entomologia Exp Applicata

Self Cite

View full text Add to dashboard Cite

Metabolomics as the study of the entire set of metabolites of a given organism is an important frontier in life sciences. As a tool that captures the 'front end' of cellular machineries, metabolomics is particularly suited to investigate biotic interactions, including for instance the interplay between plants and insects. In this review, we discuss the opportunities and challenges of metabolomics to study plantherbivore interactions. We first present a brief overview of the typical analytical workflows used in metabolomics and their associated issues, in particular those related to metabolome coverage and compound identification. Second, recent advances in the field of plant-herbivore relationships that are promoted by non-targeted approaches are reviewed, with examples ranging from classical herbivore resistance patterns to plant-mediated interactions across different spatial scales and volatile-mediated tritrophic interactions. Through general considerations and the discussion of a few selected case studies, our review highlights the potential and challenges of metabolomics as a research approach to understand biological interfaces.

show abstract

“…Then, samples can be stored frozen at −80 • C until analysis [8]. Another metabolite-quenching strategy is moisture reduction by lyophilization [46]]. Lyophilization-aka freeze-drying-eliminates most of the water needed for the development of biochemical reactions while maintaining the sample under freezing temperatures.…”

Section: Sampling and Metabolite Quenchingmentioning

confidence: 99%

Metabolomics, a Powerful Tool for Understanding Plant Abiotic Stress

et al. 2021

View full text Add to dashboard Cite

Metabolomics is a technology that generates large amounts of data and contributes to obtaining wide and integral explanations of the biochemical state of a living organism. Plants are continuously affected by abiotic stresses such as water scarcity, high temperatures and high salinity, and metabolomics has the potential for elucidating the response-to-stress mechanisms and develop resistance strategies in affected cultivars. This review describes the characteristics of each of the stages of metabolomic studies in plants and the role of metabolomics in the characterization of the response of various plant species to abiotic stresses.

show abstract

Metabolomics: Application in Plant Sciences

Cited by 9 publications

References 160 publications

Metabolomics of cereals under biotic stress: current knowledge and techniques

Metabolomics of cereals under biotic stress: current knowledge and techniques

Metabolomics in plant–herbivore interactions: challenges and applications

Metabolomics, a Powerful Tool for Understanding Plant Abiotic Stress

Contact Info

Product

Resources

About