Metabolomics changes in a rat model of obstructive jaundice: mapping to metabolism of amino acids, carbohydrates and lipids as well as oxidative stress

Long, Yunfei; Dong, Xin; Yuan, Yawei; Huang, Jinxin; Song, Jiangang; Yuan, Sun; Lu, Zhijie; Yang, Liqun; Yu, Weifeng

doi:10.3164/jcbn.14-147

Cited by 26 publications

(16 citation statements)

References 86 publications

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“…Antioxidant defenses, as demonstrated by GSH, catalase and SOD, are found to be decreased while lipid peroxidation, as demonstrated by MDA levels, is found to be increased in rat models with OJ (7,18,19). There have been studies suggesting that LA reduced MDA levels and increased antioxidant levels such as GSH and SOD in hepatic pathologies (20)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 97%

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Atalay¹,

Özdemir²,

Tur³

et al. 2019

BLL

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INTRODUCTION: The aim of this study is to investigate the effects of obstructive jaundice on the liver and effectivity of alpha-lipoic acid on liver damage and oxidative stress. MATERIALS AND METHODS: Thirty-six male Sprague-Dawley rats were divided into 3 groups per 12 animals, namely into Group I (control group): the bile duct was only mobilized by laparotomy, Group II (bile duct ligation group-BDL): the common bile duct was closed with clips and OJ was caused after laparotomy, and Group III (bile duct ligation and alpha-lipoic acid group-BDL+LA): after closing the common bile duct, LA was administered in an intramuscular dose of 50 mg/kg for 10 days. On the 10th day, malondialdehyde, glutathione and superoxide dismutase levels were measured in liver and histopathological evaluation was performed. RESULTS: AST (U/L)/ALT(U/L) in groups I, II and III were 155.33/51.83, 445.28/165.89, 380.78/173.33, respectively (p < 0.005). Superoxide dismutase and glutathione levels were lower in patient groups than in the control group (0.31 μl/g vs 0.36 μl/g; p < 0.05). After the lipoic acid treatment, none of the biochemical markers of liver improved. Only the increase in superoxide dismutase (0.31 μl/g and 0.34 μl/g in groups II and III, respectively) and glutathione levels (0.16 μl/g and 0.22 μl/g in groups II and III, respectively) was statistically signifi cant (p < 0.05). CONCLUSIONS: Histopathological damage was statistically signifi cantly decreased and antioxidant levels were statistically signifi cantly increased after LA treatment (Tab. 1, Fig. 6, Ref. 23).

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Section: Discussionmentioning

confidence: 97%

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Atalay¹,

Özdemir²,

Tur³

et al. 2019

BLL

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“…Compared with sham operated rats, BDL rats displayed oxidative stress, with diminished serum GSH, total antioxidant capacity, and superoxide dismutase and glutathione peroxidase activities, with upregulated serum malondialdehyde. Changes in certain amino acids, lipids, Krebs cycle intermediates, and lactic acid were signs of the effects of cholestasis on energy metabolism [98]. The BDL cholestasis rat model was shown to generate similar metabolic characteristics as thioacetamide (TAA)-induced cholestasis in rats, with excessive fatty acid oxidation, insufficient glutathione regeneration, and disturbed gut microbiota.…”

Section: Cholestasismentioning

confidence: 99%

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Beyoğlu

Idle

2020

Metabolites

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In recent years, there has been a plethora of attempts to discover biomarkers that are more reliable than α-fetoprotein for the early prediction and prognosis of hepatocellular carcinoma (HCC). Efforts have involved such fields as genomics, transcriptomics, epigenetics, microRNA, exosomes, proteomics, glycoproteomics, and metabolomics. HCC arises against a background of inflammation, steatosis, and cirrhosis, due mainly to hepatic insults caused by alcohol abuse, hepatitis B and C virus infection, adiposity, and diabetes. Metabolomics offers an opportunity, without recourse to liver biopsy, to discover biomarkers for premalignant liver disease, thereby alerting the potential of impending HCC. We have reviewed metabolomic studies in alcoholic liver disease (ALD), cholestasis, fibrosis, cirrhosis, nonalcoholic fatty liver (NAFL), and nonalcoholic steatohepatitis (NASH). Specificity was our major criterion in proposing clinical evaluation of indole-3-lactic acid, phenyllactic acid, N-lauroylglycine, decatrienoate, N-acetyltaurine for ALD, urinary sulfated bile acids for cholestasis, cervonoyl ethanolamide for fibrosis, 16α-hydroxyestrone for cirrhosis, and the pattern of acyl carnitines for NAFL and NASH. These examples derive from a large body of published metabolomic observations in various liver diseases in adults, adolescents, and children, together with animal models. Many other options have been tabulated. Metabolomic biomarkers for premalignant liver disease may help reduce the incidence of HCC.

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“…Val, Leu and Ile biosynthesis was observed in larvae after PCB91 exposure. Leu and Val are branched chain amino acids (BCAAs), and an appropriate ratio of BCAAs can prevent amino acid imbalance 51 . BCAAs are important amino acids in energy metabolism, and changes in BCAA levels can result in the disturbance of three major nutrients: sugar, fat and protein.…”

Section: Discussionmentioning

confidence: 99%

Chiral PCB 91 and 149 Toxicity Testing in Embryo and Larvae (Danio rerio): Application of Targeted Metabolomics via UPLC-MS/MS

Chai

Cui

Yin

et al. 2016

Sci Rep

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In this study, we aimed to investigate the dysfunction of zebrafish embryos and larvae induced by rac-/(+)-/(−)- PCB91 and rac-/(−)-/(+)- PCB149. UPLC-MS/MS (Ultra-performance liquid chromatography coupled with mass spectrometry) was employed to perform targeted metabolomics analysis, including the quantification of 22 amino acids and the semi-quantitation of 22 other metabolites. Stereoselective changes in target metabolites were observed in embryos and larvae after exposure to chiral PCB91 and PCB149, respectively. In addition, statistical analyses, including PCA and PLS-DA, combined with targeted metabolomics were conducted to identify the characteristic metabolites and the affected pathways. Most of the unique metabolites in embryos and larvae after PCB91/149 exposure were amino acids, and the affected pathways for zebrafish in the developmental stage were metabolic pathways. The stereoselective effects of PCB91/149 on the metabolic pathways of zebrafish embryos and larvae suggest that chiral PCB91/149 exposure has stereoselective toxicity on the developmental stages of zebrafish.

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Metabolomics changes in a rat model of obstructive jaundice: mapping to metabolism of amino acids, carbohydrates and lipids as well as oxidative stress

Cited by 26 publications

References 86 publications

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Chiral PCB 91 and 149 Toxicity Testing in Embryo and Larvae (Danio rerio): Application of Targeted Metabolomics via UPLC-MS/MS

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