Metabolomics for measuring phytochemicals, and assessing human and animal responses to phytochemicals, in food science

McGhie, Tony K.; Rowan, Daryl D.

doi:10.1002/mnfr.201100545

Cited by 49 publications

(34 citation statements)

References 70 publications

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“…Contemporary experimental work in the areas of food and nutrition science is significantly enhanced by the introduction of metabolomics strategies involving simultaneous analysis of many compounds [89]. Mass spectrometry and nuclear magnetic resonance are basic methods used in such analyses.…”

Section: Examples Of Databases Of Enzymes Metabolites and Metabomentioning

confidence: 99%

Internet Databases of the Properties, Enzymatic Reactions, and Metabolism of Small Molecules—Search Options and Applications in Food Science

Minkiewicz

Darewicz

Iwaniak

et al. 2016

IJMS

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Internet databases of small molecules, their enzymatic reactions, and metabolism have emerged as useful tools in food science. Database searching is also introduced as part of chemistry or enzymology courses for food technology students. Such resources support the search for information about single compounds and facilitate the introduction of secondary analyses of large datasets. Information can be retrieved from databases by searching for the compound name or structure, annotating with the help of chemical codes or drawn using molecule editing software. Data mining options may be enhanced by navigating through a network of links and cross-links between databases. Exemplary databases reviewed in this article belong to two classes: tools concerning small molecules (including general and specialized databases annotating food components) and tools annotating enzymes and metabolism. Some problems associated with database application are also discussed. Data summarized in computer databases may be used for calculation of daily intake of bioactive compounds, prediction of metabolism of food components, and their biological activity as well as for prediction of interactions between food component and drugs.

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Section: Examples Of Databases Of Enzymes Metabolites and Metabomentioning

confidence: 99%

Internet Databases of the Properties, Enzymatic Reactions, and Metabolism of Small Molecules—Search Options and Applications in Food Science

Minkiewicz

Darewicz

Iwaniak

et al. 2016

IJMS

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“…Metabolomics is the simultaneous measurement of all detectable small molecules or metabolites present in biological samples such as biofluids, tissues, and cellular extracts to elucidate the effect of a particular stimulus on metabolic pathways [22]. The use of metabolomics in nutritional sciences is gaining momentum, and global metabolomics profiling was utilized in this study to help capture the potential influence of a polyphenol- and protein-rich supplement in countering physiologic stress indicators associated with an intensified 3-day exercise training period.…”

Section: Introductionmentioning

confidence: 99%

Influence of a Polyphenol-Enriched Protein Powder on Exercise-Induced Inflammation and Oxidative Stress in Athletes: A Randomized Trial Using a Metabolomics Approach

et al. 2013

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ObjectivesPolyphenol supplementation was tested as a countermeasure to inflammation and oxidative stress induced by 3-d intensified training.MethodsWater soluble polyphenols from blueberry and green tea extracts were captured onto a polyphenol soy protein complex (PSPC). Subjects were recruited, and included 38 long-distance runners ages 19–45 years who regularly competed in road races. Runners successfully completing orientation and baseline testing (N = 35) were randomized to 40 g/d PSPC (N = 17) (2,136 mg/d gallic acid equivalents) or placebo (N = 18) for 17 d using double-blinded methods and a parallel group design, with a 3-d running period inserted at day 14 (2.5 h/d, 70% VO2max). Blood samples were collected pre- and post-14 d supplementation, and immediately and 14 h after the third day of running in subjects completing all aspects of the study (N = 16 PSPC, N = 15 placebo), and analyzed using a metabolomics platform with GC-MS and LC-MS.ResultsMetabolites characteristic of gut bacteria metabolism of polyphenols were increased with PSPC and 3 d running (e.g., hippurate, 4-hydroxyhippurate, 4-methylcatechol sulfate, 1.8-, 1.9-, 2.5-fold, respectively, P<0.05), an effect which persisted for 14-h post-exercise. Fatty acid oxidation and ketogenesis were induced by exercise in both groups, with more ketones at 14-h post-exercise in PSPC (3-hydroxybutyrate, 1.8-fold, P<0.05). Established biomarkers for inflammation (CRP, cytokines) and oxidative stress (protein carbonyls) did not differ between groups.ConclusionsPSPC supplementation over a 17-d period did not alter established biomarkers for inflammation and oxidative stress but was linked to an enhanced gut-derived phenolic signature and ketogenesis in runners during recovery from 3-d heavy exertion.Trial RegistrationClinicalTrials.gov, U.S. National Institutes of Health, identifier: NCT01775384

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“…This might not be the case for untargeted analysis due to the need to target as many metabolites as possible. Extraction techniques commonly used for fermented foods are solvents (methanol, chloroform, ethanol, acetonitrile) [27,28,32], similar to those used in other metabolomics studies [15,17,24,31]. When the focus of the study is on specific nonvolatile metabolites, derivatization may be required prior to analysis on GC-MS.…”

Section: Extractionmentioning

confidence: 99%

Food Metabolomics: A New Frontier in Food Analysis and its Application to Understanding Fermented Foods

Adebo¹,

Njobeh²,

AdeyinkaAdebiyi³

et al. 2017

Functional Food - Improve Health Through Adequate Food

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The emergence of food metabolomics, otherwise known as foodomics, has opened new frontiers and possibilities for scientists to characterize and simultaneously determine and obtain the comprehensive profile of the food metabolome. Qualitative and quantitative determinations of this metabolome offer insights into the underlying processes involved and details about the content of the food analytes. This had seemed technically challenging and impossible over time, but can now be done due to the advent of sophisticated analytical equipment and chemometric tools. The application of this technique offers enormous opportunities to obtain detailed information that can be correlated to various properties, functionalities and potentials in fermented foods. This chapter thus evaluated and documented studies presented in the literature on the food metabolomics study of fermented foods, with a view of appraising its prospects, applications and subsequent utilization in the study of fermented foods.

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Metabolomics for measuring phytochemicals, and assessing human and animal responses to phytochemicals, in food science

Cited by 49 publications

References 70 publications

Internet Databases of the Properties, Enzymatic Reactions, and Metabolism of Small Molecules—Search Options and Applications in Food Science

Internet Databases of the Properties, Enzymatic Reactions, and Metabolism of Small Molecules—Search Options and Applications in Food Science

Influence of a Polyphenol-Enriched Protein Powder on Exercise-Induced Inflammation and Oxidative Stress in Athletes: A Randomized Trial Using a Metabolomics Approach

Food Metabolomics: A New Frontier in Food Analysis and its Application to Understanding Fermented Foods

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