Metabolomics of tracheal wash samples and exhaled breath condensates in healthy horses and horses affected by equine asthma

Bazzano, Marilena; Laghi, Luca; Zhu, Chenglin; Magi, Gian Enrico; Serri, Evelina; Spaterna, Andrea; Tesei, Beniamino; Laus, Fulvio

doi:10.1088/1752-7163/aade13

Cited by 23 publications

(24 citation statements)

References 73 publications

(113 reference statements)

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“…In agreement with Bazzano et al [51], we highlighted any trend characterizing the samples with robust principal component analysis (rPCA) models [52], using the molecules accepted by univariate analysis as a base. We took advantage of the PcaHubert algorithm implemented in the "rrcov" package.…”

Section: Discussionsupporting

confidence: 90%

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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The urine from 35 giraffes was studied by untargeted 1H-NMR, with the purpose of obtaining, for the first time, a fingerprint of its metabolome. The metabolome, as downstream of the transcriptome and proteome, has been considered as the most representative approach to monitor the relationships between animal physiological features and environment. Thirty-nine molecules were unambiguously quantified, able to give information about diet, proteins digestion, energy generation, and gut-microbial co-metabolism. The samples collected allowed study of the effects of age and sex on the giraffe urinary metabolome. In addition, preliminary information about how sampling procedure and pregnancy could affect a giraffe’s urinary metabolome was obtained. Such work could trigger the setting up of methods to non-invasively study the health status of giraffes, which is utterly needed, considering that anesthetic-related complications make their immobilization a very risky practice.

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Section: Discussionsupporting

confidence: 90%

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

et al. 2020

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“…The same result was recently found in the saliva of soccer players, and the change was ascribed to the higher evaporation of volatile compounds in the mouth during exercise [21]. In a recent study, methanol was considered a marker of inflammation in horses affected by equine asthma when detected in exhaled breath condensate (EBC) [40].…”

Section: Discussionsupporting

confidence: 63%

Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR

et al. 2020

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In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites’ profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T0) and immediately after (T1) standardized exercise. Metabolomic analysis of both samples was performed by 1H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T0 and T1 in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses.

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“…Additionally, both hydrogen peroxide and pH had a positive association with BAL neutrophil percentage, while leukotriene B-4 demonstrated a positive association with BAL eosinophil percentage. Another study characterized the metabolomic profile of tracheal wash and exhaled breath condensate in healthy horses and those with severe EA (172). Higher concentrations of histamine and oxidant agents, such as glutamate, valine, leucine, and isoleucine, as well as lower levels of ascorbate, methylamine, dimethylamine and O-phosphocholine, were found in the group of severe EA, compared to healthy controls.…”

Section: Are Pertinent Biomarkers Of Equine Asthma Already Available mentioning

confidence: 99%

Equine Asthma: Current Understanding and Future Directions

et al. 2020

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The 2019 Havemeyer Workshop brought together researchers and clinicians to discuss the latest information on Equine Asthma and provide future research directions. Current clinical and molecular asthma phenotypes and endotypes in humans were discussed and compared to asthma phenotypes in horses. The role of infectious and non-infectious causes of equine asthma, genetic factors and proposed disease pathophysiology were reviewed. Diagnostic limitations were evident by the limited number of tests and biomarkers available to field practitioners. The participants emphasized the need for more accessible, standardized diagnostics that would help identify specific phenotypes and endotypes in order to create more targeted treatments or management strategies. One important outcome of the workshop was the creation of the Equine Asthma Group that will facilitate communication between veterinary practice and research communities through published and easily accessible guidelines and foster research collaboration.

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Metabolomics of tracheal wash samples and exhaled breath condensates in healthy horses and horses affected by equine asthma

Cited by 23 publications

References 73 publications

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy

Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR

Equine Asthma: Current Understanding and Future Directions

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