Metabolomics reveals the intervention effect of Zhuang medicine Longzuantongbi granules on a collagen-induced arthritis rat model by using UPLC-MS/MS

Yao, Lan; Cheng, Shuohan; Yang, Jun; Xiang, Fangfang; Zhou, Zhi; Zhang, Qinghuai; Pang, Yuzhou; Zhou, Wuduo; Abliz, Zeper

doi:10.1016/j.jep.2022.115325

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…The identified metabolites in metabolomics were then mapped to the KEGG pathway for biological interpretation of higher-level systemic functions. The metabolites and corresponding pathways were visualized using the KEGG Mapper tool 16 .…”

Section: Methodsmentioning

confidence: 99%

Integration of multiomics analysis to reveal the major pathways of vitamin A deficiency aggravates acute respiratory distress syndrome in neonatal rats

Tang,

Yuan,

Sun

et al. 2023

Sci Rep

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Acute respiratory distress syndrome (ARDS) is a major disease that threatens the life and health of neonates. Vitamin A (VA) can participate in early fetal lung development and affect lung immune function. Researches revealed that the serum VA level in premature infants with ARDS was lower than that in premature infants without ARDS of the same gestational age, and premature infants with VA deficiency (VAD) were more likely to develop ARDS. Moreover, the VA levels can be used as a predictor of the development and severity of neonatal ARDS. However, the critical question here is; Does ARDS develop due to VAD in these systemic diseases? Or does ARDS develop because these diseases cause VAD? We hypothesize that VAD may aggravate neonatal ARDS by affecting immunity, metabolism, barriers and other pathways. In this article, we used multiomics analysis to find that VAD may aggravate ARDS mainly through the Fc epsilon RI signaling pathway, the HIF-1 signaling pathway, glutathione metabolism, and valine, leucine and isoleucine degradation signaling pathways, which may provide the molecular pathogenic mechanism behind the pathology of VAD-aggravated ARDS and can also provide potential molecular targets for subsequent research on ARDS.

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Section: Methodsmentioning

confidence: 99%

Integration of multiomics analysis to reveal the major pathways of vitamin A deficiency aggravates acute respiratory distress syndrome in neonatal rats

Tang,

Yuan,

Sun

et al. 2023

Sci Rep

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“…A Waters ACQUITY UPLC HSST3 column (1.8 μm, 2.1 × 100 mm) was used for chromatographic separation, and the mobile phase consisted of 0.1% formic acid aqueous solution (phase A) and 0.1% formic acidacetonitrile (phase B) at a flow rate of 0.2 ml/min. Gradient elution conditions were properly adjust according to literature ( Yao et al, 2022 ), as follows: 0–2 min, 99%–99% A; 2–3 min, 99%–65% A; 3–25 min, 60%–1% A; 25–30 min, 1% A; 30–30 min, 1%–99% A; 30–40 min, 99%–99% A. The total chromatographic run time was 40 min, the injection volume was 5 μL, and the column temperature was 35°C.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin

Xiao

Xie

et al. 2022

Front. Pharmacol.

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Gypenosides (GYP) exerted anticancer activity against various cancers. However, the mechanism of GYP against lung cancer (LC) in vivo remains unclear. This study aims to reveal the potential mechanism of GYP against LC and enhancing cisplatin efficacy using a comprehensive analysis of metabolomics, network analysis. Pharmacodynamic results showed that GYP inhibited tumor growth, reduced tumor volume and tumor weight, and alleviated pathological symptoms in Lewis tumor-bearing mice, and GYP could enhance the anti-LC effects of cisplatin. Using serum metabolomics methods, 53 metabolites were found to be significantly altered in the model group, and the levels of 23 biomarkers were significantly restored after GYP treatment. GYP-related metabolic pathways involved six pathways, including alpha-linolenic acid metabolism, glutathione metabolism, sphingolipid metabolism, glycerophospholipid metabolism, tryptophan metabolism, and primary bile acid biosynthesis. 57 genes associated with differential metabolites of GYP recovery and 7 genes of 11 saponins of GYP against LC were screened by network analysis, the STRING database was used to find the association between 57 genes and 7 genes, and a compound-intersection gene-metabolite related gene-metabolite-pathway network was constructed, and STAT3, MAPK14, EGFR and TYMS might be the crucial targets of GYP against LC. Western blot results showed that GYP restored the levels of STA3, MAPK14, EGFR, and TYMS in the model group, and GYP also restored the levels of STAT3 and MAPK14 in the cisplatin group, indicating that GYP might exert anti-LC effects and enhance the pharmacological effects of cisplatin through MAPK14/STAT3 signaling pathway. Our method revealed the effect and mechanism of GYP on LC and the pharmacological effects of GYP-enhanced chemotherapeutic agent cisplatin, which provided some reference for the development of anti-cancer drugs.

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“…He et al (2022) studied the role of Jigucao capsule (JGCC) in the treatment of Dampnessheat Jaundice syndrome (DHJS) using a classical strategy, which is a research technique of UPLC-QTOF-MS united with pattern identification along with metabolomics applications and digital databases JGCC has been found to call back twenty-five potential biomarkers, including arachidonic acid, L-urobilin, etc. Yao et al (2022) used UPLC-QTOF-MS to find that Longzuantongbi granules effectively regulated 11 biomarkers associated with lipid metabolism and amino acid metabolism, such as lysophosphatidylcholine, alpha-3-hydroxybutyric acid, alpha-linolenic acid, arachidonic acid, and 12-HETE. Wang et al (2021) demonstrated that Liu Shen capsule treats respiratory diseases by altering airway microbiota, such as Bacteroidetes and Proteobacteria, based on UPLC-QTOF-MS. Yang et al (2022) explored the antidiabetic effect of red ginseng on type Ⅱ diabetes with a focus on UPLC-Q Exactive-MS Technology and found that disturbances in regulatory pathways such as D-arginine and D-ornithine metabolic activities, tryptophan bioconversion, taurine, and hypotaurine metabolism were improved after red ginseng intervention.…”

Section: Pharmacodynamic Evaluationmentioning

confidence: 99%

Mass spectrometry-based metabolomics for discovering active ingredients and exploring action mechanism of herbal medicine

et al. 2023

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Natural products derived from herbal medicine are a fruitful source of lead compounds because of their structural diversity and potent bioactivities. However, despite the success of active compounds derived from herbal medicine in drug discovery, some approaches cannot effectively elucidate the overall effect and action mechanism due to their multi-component complexity. Fortunately, mass spectrometry-based metabolomics has been recognized as an effective strategy for revealing the effect and discovering active components, detailed molecular mechanisms, and multiple targets of natural products. Rapid identification of lead compounds and isolation of active components from natural products would facilitate new drug development. In this context, mass spectrometry-based metabolomics has established an integrated pharmacology framework for the discovery of bioactivity-correlated constituents, target identification, and the action mechanism of herbal medicine and natural products. High-throughput functional metabolomics techniques could be used to identify natural product structure, biological activity, efficacy mechanisms, and their mode of action on biological processes, assisting bioactive lead discovery, quality control, and accelerating discovery of novel drugs. These techniques are increasingly being developed in the era of big data and use scientific language to clarify the detailed action mechanism of herbal medicine. In this paper, the analytical characteristics and application fields of several commonly used mass spectrometers are introduced, and the application of mass spectrometry in the metabolomics of traditional Chinese medicines in recent years and its active components as well as mechanism of action are also discussed.

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Metabolomics reveals the intervention effect of Zhuang medicine Longzuantongbi granules on a collagen-induced arthritis rat model by using UPLC-MS/MS

Cited by 13 publications

References 35 publications

Integration of multiomics analysis to reveal the major pathways of vitamin A deficiency aggravates acute respiratory distress syndrome in neonatal rats

Integration of multiomics analysis to reveal the major pathways of vitamin A deficiency aggravates acute respiratory distress syndrome in neonatal rats

Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin

Mass spectrometry-based metabolomics for discovering active ingredients and exploring action mechanism of herbal medicine

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