Metabonomic Profiles Delineate the Effect of Traditional Chinese Medicine Sini Decoction on Myocardial Infarction in Rats

Abstract

BackgroundIn spite of great advances in target-oriented Western medicine for treating myocardial infarction (MI), it is still a leading cause of death in a worldwide epidemic. In contrast to Western medicine, Traditional Chinese medicine (TCM) uses a holistic and synergistic approach to restore the balance of Yin-Yang of body energy so the body's normal function can be restored. Sini decoction (SND) is a well-known formula of TCM which has been used to treat MI for many years. However, its holistic activity evaluation and mechanistic understanding are still lacking due to its complex components.Methodology/Principal FindingsA urinary metabonomic method based on nuclear magnetic resonance and ultra high-performance liquid chromatography coupled to mass spectrometry was developed to characterize MI-related metabolic profiles and delineate the effect of SND on MI. With Elastic Net for classification and selection of biomarkers, nineteen potential biomarkers in rat urine were screened out, primarily related to myocardial energy metabolism, including the glycolysis, citrate cycle, amino acid metabolism, purine metabolism and pyrimidine metabolism. With the altered metabolism pathways as possible drug targets, we systematically analyze the therapeutic effect of SND, which demonstrated that SND administration could provide satisfactory effect on MI through partially regulating the perturbed myocardial energy metabolism.Conclusions/SignificanceOur results showed that metabonomic approach offers a useful tool to identify MI-related biomarkers and provides a new methodological cue for systematically dissecting the underlying efficacies and mechanisms of TCM in treating MI.

Myocardial lipidomics profiling delineate the toxicity of traditional Chinese medicine Aconiti Lateralis radix praeparata

“…Data processing used the method previously published by our group with minor modifications (Tan et al, 2012). The raw data in instrument specific format (.d) were firstly converted to common data format (.mzdata) files by a conversion software program, in which the isotope interferences were excluded.…”

UHPLC-MS for the analytical characterization of traditional Chinese medicines

“…Metabolomics seeks to elucidate the therapeutic properties and metabolism of prescription drugs and the metabolic pathways involved [81][82][83][84][85][86][87][88][89][90][91][92]. It will greatly facilitate understanding of the mechanisms of action of TCMs [93][94][95][96][97][98][99][100][101][102].…”

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“…It has been recognized that TCM effectively treats symptoms, reduce side effects and reverse resistance via targeting multiple pathways. TCMs such as Shengmai [9], Sini decoction [10], Shuanglong formula [11], and Huangqi injection have been reported to exhibit potential pharmacological activities against cardiovascular diseases [12]. Draba nemorosa stimulates blood circulation, thus eliminating blood stasis.…”