Metagenomic Insights into Microbial Diversity and Metabolic Potential of Hot Spring Ecosystems

Saini, Jasleen; Kaur, Ramanpreet; Sharma, Pushpender Kumar

doi:10.1007/978-981-10-5708-3_9

Cited by 2 publications

(5 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of postculturing, the viable values of bacterial count generally range from <1% or to >10%, which makes culturing protocols less reliable in determining the diversity of bioaerosols. 47 The emergence of molecular approaches such as metagenomics has nonetheless aided the identification of novel bacterial species in recent times. 47 The quantification of bioaerosol samples collected from atmospheric specimens can be achieved generally with considerably large volumes of air (e.g., >100 m 3 ) owing to the low concentration of microbial cells in the atmosphere (∼10 2 to ∼10 6 cells m −3 of air).…”

Section: And Monitoringmentioning

confidence: 99%

“…47 The emergence of molecular approaches such as metagenomics has nonetheless aided the identification of novel bacterial species in recent times. 47 The quantification of bioaerosol samples collected from atmospheric specimens can be achieved generally with considerably large volumes of air (e.g., >100 m 3 ) owing to the low concentration of microbial cells in the atmosphere (∼10 2 to ∼10 6 cells m −3 of air). 38,44 This low-biomass condition may further complicate the subsequent quantification of the bioaerosols through the contaminations in sampling systems.…”

Section: And Monitoringmentioning

confidence: 99%

“…It is commonly acknowledged that the majority of the microbial aerosols cannot be cultured owing to the absence of appropriate culturing techniques or loss of biomass during the culturing process. In the case of postculturing, the viable values of bacterial count generally range from <1% or to >10%, which makes culturing protocols less reliable in determining the diversity of bioaerosols . The emergence of molecular approaches such as metagenomics has nonetheless aided the identification of novel bacterial species in recent times …”

Section: Considerations For Bioaerosol Sampling and Monitoringmentioning

confidence: 99%

Section: Considerations For Bioaerosol Sampling and Monitoringmentioning

confidence: 99%

See 3 more Smart Citations

Recent Advances in Monitoring, Sampling, and Sensing Techniques for Bioaerosols in the Atmosphere

et al. 2020

View full text Add to dashboard Cite

Bioaerosols in the form of microscopic airborne particles pose pervasive risks to humans and livestock. As either fully active components (e.g., viruses, bacteria, and fungi) or as whole or part of inactive fragments, they are among the least investigated pollutants in nature. Their identification and quantification are essential to addressing related dangers and to establishing proper exposure thresholds. However, difficulties in the development (and selection) of detection techniques and an associated lack of standardized procedures make the sensing of bioaerosols challenging. Through a comprehensive literature search, this review examines the mechanisms of conventional and advanced bioaerosol detection methods. It also provides a roadmap for future research and development in the selection of suitable methodologies for bioaerosol detection. The development of sample collection and sensing technology make it possible for continuous and automated operation. However, intensive efforts should be put to overcome the limitations of current technology as most of the currently available options tend to suffer from lengthy sample acquisition times and/or nonspecificity of probe material.

show abstract

Section: And Monitoringmentioning

confidence: 99%

Section: And Monitoringmentioning

confidence: 99%

Section: Considerations For Bioaerosol Sampling and Monitoringmentioning

confidence: 99%

Section: Considerations For Bioaerosol Sampling and Monitoringmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in Monitoring, Sampling, and Sensing Techniques for Bioaerosols in the Atmosphere

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Microorganisms that thrive in these extreme temperature, flow rate, and fluid compositions include bacteria, archaea, phages, and eukaryotes (e.g., diatoms) (Skirnisdottir et al, 2000;Breitbart et al, 2004;Leira et al, 2017;Saini et al, 2017;Pumas et al, 2018), and the cells of these organisms can also be entombed by silica precipitation, preserving both the physical structure of the cell and cellular organic matter (Cady and Farmer, 1996). These domains of life inhabit specific microenvironments at the hot springs conducive to the organisms' ideal growth conditions.…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Preservation in Modern and Relict Hot-Spring Deposits in Iceland, with Implications for Organics Detection on Mars

et al. 2021

View full text Add to dashboard Cite

Hydrothermal spring deposits host unique microbial ecosystems and have the capacity to preserve microbial communities as biosignatures within siliceous sinter layers. This quality makes terrestrial hot springs appealing natural laboratories to study the preservation of both organic and morphologic biosignatures. The discovery of hydrothermal deposits on Mars has called attention to these hot springs as Mars-analog environments, driving forward the study of biosignature preservation in these settings to help prepare future missions targeting the recovery of biosignatures from martian hot-spring deposits. This study quantifies the fatty acid load in three Icelandic hot-spring deposits ranging from modern and inactive to relict. Samples were collected from both the surface and 2-18 cm in depth to approximate the drilling capabilities of current and upcoming Mars rovers. To determine the preservation potential of organics in siliceous sinter deposits, fatty acid analyses were performed with pyrolysis-gas chromatography-mass spectrometry (GC-MS) utilizing thermochemolysis with tetramethylammonium hydroxide (TMAH). This technique is available on both current and upcoming Mars rovers. Results reveal that fatty acids are often degraded in the subsurface relative to surface samples but are preserved and detectable with the TMAH pyrolysis-GC-MS method. Hot-spring mid-to-distal aprons are often the best texturally and geomorphically definable feature in older, degraded terrestrial sinter systems and are therefore most readily detectable on Mars from orbital images. These findings have implications for the detection of organics in martian hydrothermal systems as they suggest that organics might be detectable on Mars in relatively recent hot-spring deposits, but preservation likely deteriorates over geological timescales. Rovers with thermochemolysis pyrolysis-GC-MS instrumentation may be able to detect fatty acids in hotspring deposits if the organics are relatively young; therefore, martian landing site and sample selection are of paramount importance in the search for organics on Mars.

show abstract

Metagenomic Insights into Microbial Diversity and Metabolic Potential of Hot Spring Ecosystems

Cited by 2 publications

References 96 publications

Recent Advances in Monitoring, Sampling, and Sensing Techniques for Bioaerosols in the Atmosphere

Recent Advances in Monitoring, Sampling, and Sensing Techniques for Bioaerosols in the Atmosphere

Fatty Acid Preservation in Modern and Relict Hot-Spring Deposits in Iceland, with Implications for Organics Detection on Mars

Contact Info

Product

Resources

About