Metal(loid) speciation and transformation by aerobic methanotrophs

Karthikeyan, Obulisamy Parthiba; Smith, Thomas J.; Dandare, Shamsudeen Umar; Parwin, Kamaludeen Sara; Singh, Heetasmin; Loh, Hui Xin; Cunningham, Mark R.; Williams, Paul N.; Nichol, Tim; Subramanian, Avudainayagam; Ramasamy, Kumarasamy; Kumaresan, Deepak

doi:10.1186/s40168-021-01112-y

Cited by 15 publications

(7 citation statements)

References 156 publications

(192 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cd is very toxic and probably carcinogenic at low concentrations because of its very long half-life (Xu et al, 2020). Hg is a global health threat and causes impairments in the human nervous system (Karthikeyan et al, 2021). Many terrible diseases such as Minamata and Parkinson's disease are related to the overexposure and excessive accumulation of mercury (Cai and Wang, 2022).…”

Section: Introductionmentioning

confidence: 99%

One-step synthesis of highly fluorescent carbon dots as fluorescence sensors for the parallel detection of cadmium and mercury ions

Tan

Wang

et al. 2022

Front. Chem.

View full text Add to dashboard Cite

Cadmium (Cd2+) and mercury ions (Hg2+) are essential for the quality control of food samples because of their serious toxicity to human health, but the effective and simple strategy for their parallel detection remains challenging. In this paper, a rapid and simple parallel detection method for Cd2+ and Hg2+ was developed using carbon dots (CDs) as fluorescent sensors. A one-step hydrothermal method with a single precursor l-arginine as both the carbon and nitrogen sources was employed to prepare nitrogen-doped CDs (N-CDs). N-CDs exhibited a uniform particle size and excitation-independent fluorescence emission. The maximum emission wavelength of N-CDs was observed at 354 nm with the excitation wavelength at 295 nm. The quantum yield of N-CDs reached as high as 71.6% in water. By using sodium diphosphate and phytic acid as masking agents, the fluorescent sensor can be quenched by Cd2+ and Hg2+ in the linear range of 0–26.8 μM and 0–49.9 μM within 5 min. Other common ions in farm products showed no significant effect on the fluorescence intensity of the sensing system. The results demonstrated that the sensing system had good selectivity and sensitivity for Cd2+ and Hg2+. The detection limits for Cd2+ and Hg2+ were 0.20 and 0.188 μM, respectively. In addition, the fluorescent sensor had been successfully applied for the detection of Cd2+ and Hg2+ in fruits and vegetables, and the recoveries were 86.44–109.40% and 86.62–115.32%, respectively. The proposed fluorescent sensor provides a rapid, simple, and sensitive detection method for Cd2+ and Hg2+ in food samples and thus a novel quantitative detection method for heavy metal ions in foods.

show abstract

Section: Introductionmentioning

confidence: 99%

One-step synthesis of highly fluorescent carbon dots as fluorescence sensors for the parallel detection of cadmium and mercury ions

Tan

Wang

et al. 2022

Front. Chem.

View full text Add to dashboard Cite

show abstract

“…In methanotrophs, the uptake and utilization of Zn 2+ is a complex process that involves the action of transporters and regulatory proteins. Zn 2+ uptake could be dependent on a similar transport system to Mb …”

Section: Resultsmentioning

confidence: 99%

High Cell-Density Cultivation of Methylococcus capsulatus Bath for Efficient Methane-Derived Mevalonate Production

Jang

Jeong

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

The engineered Methylococcus capsulatus Bath presents a promising approach for converting methane, a potent greenhouse gas, into valuable chemicals. High cell-density culture (HCDC) is necessary for high-titer growth-associated bioproducts, but it often requires time-consuming and labor-intensive optimization processes. In this study, we aimed to achieve efficient HCDC of M. capsulatus Bath by measuring the residual nutrient levels during bioreactor operations and analyzing the specific uptake of each medium component. By controlling the concentrations of nutrients, particularly calcium and phosphorus via intermittent feeding, we achieved a high cell density of 28.2 g DCW/L and a significantly elevated production of mevalonate at a concentration of 1.8 g/L from methane. Our findings demonstrate that the methanotroph HCDC approach presented herein offers a promising strategy for promoting sustainable development, with an exceptional g-scale production titer for value-added synthetic biochemicals.

show abstract

“…Unlike the BSC in previous studies [ 7 , 8 ], the BAC investigated in this study showed the characteristics of a sponge-like porous structure and the functions of metal(loid) immobilization in aquatic habitats. The BAC has a potential to improve the quality of metal(loid)-contaminated water bodies and has, therefore, been considered a suitable candidate for investigations of microbial interactions with minerals and metal(loid)s derived from mining activities [ 1 , 9 , 10 ]. However, our understanding of metal(loid) bioavailability and microbial communities in BAC of polymetallic water bodies remains limited.…”

Section: Introductionmentioning

confidence: 99%

Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm

et al. 2023

View full text Add to dashboard Cite

Background Understanding the ecological and environmental functions of phototrophic biofilms in the biological crust is crucial for improving metal(loid) (e.g. Cd, As) bioremediation in mining ecosystems. In this study, in combination with metal(loid) monitoring and metagenomic analysis, we systematically evaluated the effect of biofilm in a novel biological aqua crust (biogenic aqua crust—BAC) on in situ metal(loid) bioremediation of a representative Pb/Zn tailing pond. Results We observed strong accumulation of potentially bioavailable metal(loid)s and visible phototrophic biofilms in the BAC. Furthermore, dominating taxa Leptolyngbyaceae (10.2–10.4%, Cyanobacteria) and Cytophagales (12.3–22.1%, Bacteroidota) were enriched in biofilm. Along with predominant heterotrophs (e.g. Cytophagales sp.) as well as diazotrophs (e.g. Hyphomonadaceae sp.), autotrophs/diazotrophs (e.g. Leptolyngbyaceae sp.) in phototrophic biofilm enriched the genes encoding extracellular peptidase (e.g. family S9, S1), CAZymes (e.g. CBM50, GT2) and biofilm formation (e.g. OmpR, CRP and LuxS), thus enhancing the capacity of nutrient accumulation and metal(loid) bioremediation in BAC system. Conclusions Our study demonstrated that a phototrophic/diazotrophic biofilm constitutes the structured communities containing specific autotrophs (e.g. Leptolyngbyaceae sp.) and heterotrophs (e.g. Cytophagales sp.), which effectively control metal(loid) and nutrient input using solar energy in aquatic environments. Elucidation of the mechanisms of biofilm formation coupled with metal(loid) immobilization in BAC expands the fundamental understanding of the geochemical fate of metal(loid)s, which may be harnessed to enhance in situ metal(loid) bioremediation in the aquatic ecosystem of the mining area.

show abstract

Metal(loid) speciation and transformation by aerobic methanotrophs

Cited by 15 publications

References 156 publications

One-step synthesis of highly fluorescent carbon dots as fluorescence sensors for the parallel detection of cadmium and mercury ions

One-step synthesis of highly fluorescent carbon dots as fluorescence sensors for the parallel detection of cadmium and mercury ions

High Cell-Density Cultivation of Methylococcus capsulatus Bath for Efficient Methane-Derived Mevalonate Production

Novel biological aqua crust enhances in situ metal(loid) bioremediation driven by phototrophic/diazotrophic biofilm

Contact Info

Product

Resources

About