Metal–organic framework-interfaced ELISA probe enables ultrasensitive detection of extracellular vesicle biomarkers

Shi, Jingzhu; Barman, Sharat Chandra; Cheng, Shibo; Zeng, Yong

doi:10.1039/d4tb00585f

J. Mater. Chem. B

2024

DOI: 10.1039/d4tb00585f

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Metal–organic framework-interfaced ELISA probe enables ultrasensitive detection of extracellular vesicle biomarkers

Jingzhu Shi,

Sharat Chandra Barman,

Shibo Cheng

et al.

Abstract: The enzyme-linked immunosorbent assay (ELISA) stands as the prevailing method for quantifying protein biomarkers. Enzymatic signal generation and amplification is a key mechanism that governs its analytical performance. This study...

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Detection of AFB1 by Immunochromatographic Test Strips Based on Double-Probe Signal Amplification with Nanobody and Biotin–Streptavidin System

Li,

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Aflatoxin B1 (AFB1) is highly toxic and difficult to prevent. It is mainly produced by fungi and exists in plants and animals and is classified by the World Health Organization as a class I carcinogen, posing a serious threat to human and animal health. Therefore, it is important to establish an efficient, sensitive, and on-site detection method for AFB1 to protect human health. The immunochromatographic test strip method is simple, sensitive, and can achieve real-time detection. However, traditional immunochromatographic test strips have low sensitivity due to their relatively weak optical properties. In this study, Nb-G8 was biotinylated using a chemical method. Two sizes of gold nanoflowers (AuNFs) were prepared and combined with biotinylated G8 and streptavidin to form two types of probes. These probes were sprayed on gold standard pads and expanded pads, respectively, to enhance the signals through the high affinity interaction between streptavidin and biotin. Under the optimal experimental conditions, the half maximal inhibitory concentration (IC50) of this method was 5.0 ng/mL and the limit of detection (IC10) was 0.03 ng/mL, which increased the sensitivity of the test strip by four-fold compared with that of the traditional biotinylated nanoantibody immunochromatography test strip and had a wider detection range. In conclusion, the use of a high-affinity amplification signal between biotin and streptavidin is a valuable method for the detection of aflatoxin.

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Metal–organic framework-interfaced ELISA probe enables ultrasensitive detection of extracellular vesicle biomarkers

Abstract: The enzyme-linked immunosorbent assay (ELISA) stands as the prevailing method for quantifying protein biomarkers. Enzymatic signal generation and amplification is a key mechanism that governs its analytical performance. This study...

Cited by 2 publications

References 55 publications

Current status and prospects of detection of breast cancer by MOFs platform

Current status and prospects of detection of breast cancer by MOFs platform

Detection of AFB1 by Immunochromatographic Test Strips Based on Double-Probe Signal Amplification with Nanobody and Biotin–Streptavidin System

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