Metal-organic frameworks with different dimensionalities: An ideal host platform for enzyme@MOF composites

Du, Yingjie; Jia, Xi; Zhong, Le; Jiao, Yi; Zhang, Zhijin; Wang, Ziyuan; Feng, Yuxiao; Bilal, Muhammad; Cui, Jiandong; Jia, Shiru

doi:10.1016/j.ccr.2021.214327

Cited by 166 publications

(89 citation statements)

References 101 publications

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“…8 On the other hand, metal−organic frameworks (MOFs) are an emerging class of porous materials that are ideal substrates for the development of carrier-immobilized CAs due to their good pore structure and tunable porosity, good biocompatibility, good chemical/mechanical stability, and high specific surface area. 9,10 Ren et al prepared a combined immobilization system of CA and ZIF-8 with a cruciate flower-like morphology by adsorbing CA onto ZIF-8, which retained 85% of its original activity after nine cycles. 11 However, they were hindered by the complex and costly immobilization process or carriers.…”

Section: ■ Introductionmentioning

confidence: 99%

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Liu

Yuan

et al. 2022

ACS Sustainable Chem. Eng.

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Carbon dioxide capture, conversion, and utilization with carbonic anhydrase (CA) as a green and sustainable method still faces the challenges of complex and costly purification, relatively low yield, and poor catalytic performances and reusability. Herein, we proposed an all-in-one strategy to solve these problems by self-assembling nanosized CA oligomeric particles (nCAOPs) into micrometer-sized CA supraparticles (mCASPs) with well-designed tags. The preparation of the purified mCASPs was simple and effective by an easy scalable low-speed centrifugation method with 78% activity recovery. The obtained mCASPs with a yield of 870 mg/L was the reported highest yield of CAs. Interestingly, mCASPs could serve as carrier-free immobilized CAs and retain over 90% original activity after 15 reuse cycles. More encouragingly, mCASPs could redissolve and form nCAOPs, which had excellent catalytic performances. The hydrated activity of nCAOPs was 1.05 times that of free CAs. Also, the k cat/K m value was 1.74 times that of free CAs, and the half-life at 40 °C was 3.83 times that of free CAs. Due to the simplicity of purification and immobilization, high yield, and excellent enzymatic properties, mCASPs and nCAOPs are considered novel bioactive materials, which offer the feasibility of CAs for sustainable CO2 capture to achieve the target of carbon neutrality.

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Section: ■ Introductionmentioning

confidence: 99%

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Liu

Yuan

et al. 2022

ACS Sustainable Chem. Eng.

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“…Dimartino et al [ 60 ] explored a suite of formulations including bifunctional acrylate monomers, diacrylate and tetraacrylate crosslinkers, as well as fatty alcohol porogens, for the fabrication of ordered porous beds for diverse chromatography and bioreactor applications ( Figure 4 B). In recent years, metal organic frameworks (MOFs) have been widely used for enzyme immobilization [ 65 ]. Limitations on making macroscopic structures with MOFs can be overcome with 3D printing.…”

Section: Post-printing Immobilizationmentioning

confidence: 99%

“…Importantly, though our discussion was confined to studies with enzymes directly immobilized on or inside the 3D printed structure, further progress in this convergence field is not separable from the independently evolving fields of 3D printing [ 16 ], conventional enzyme immobilization [ 65 ], 3D printed microfluidic devices [ 96 ], and bioprinting [ 35 ]. Extensive literature exists on employing 3D printing methods in biomedical engineering field [ 97 ] for live cell cultures and delivering bioactive molecules.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Advances in 3D Gel Printing for Enzyme Immobilization

Shen

Zhang

Fang

et al. 2022

Gels

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Incorporating enzymes with three-dimensional (3D) printing is an exciting new field of convergence research that holds infinite potential for creating highly customizable components with diverse and efficient biocatalytic properties. Enzymes, nature’s nanoscale protein-based catalysts, perform crucial functions in biological systems and play increasingly important roles in modern chemical processing methods, cascade reactions, and sensor technologies. Immobilizing enzymes on solid carriers facilitates their recovery and reuse, improves stability and longevity, broadens applicability, and reduces overall processing and chemical conversion costs. Three-dimensional printing offers extraordinary flexibility for creating high-resolution complex structures that enable completely new reactor designs with versatile sub-micron functional features in macroscale objects. Immobilizing enzymes on or in 3D printed structures makes it possible to precisely control their spatial location for the optimal catalytic reaction. Combining the rapid advances in these two technologies is leading to completely new levels of control and precision in fabricating immobilized enzyme catalysts. The goal of this review is to promote further research by providing a critical discussion of 3D printed enzyme immobilization methods encompassing both post-printing immobilization and immobilization by physical entrapment during 3D printing. Especially, 3D printed gel matrix techniques offer mild single-step entrapment mechanisms that produce ideal environments for enzymes with high retention of catalytic function and unparalleled fabrication control. Examples from the literature, comparisons of the benefits and challenges of different combinations of the two technologies, novel approaches employed to enhance printed hydrogel physical properties, and an outlook on future directions are included to provide inspiration and insights for pursuing work in this promising field.

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“…Several authors have published reviews that discuss biocatalysts composed of enzymes and MOFs, addressing the most common methods of synthesis and immobilization of these composites, as well as their several applications [ 86 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 ]. In this scenario, this review intends to update the discussion of MOFs as highly relevant materials for a wide range of applications, as well as to discuss their roles and mechanisms of action as supports for enzymatic immobilization, and the different combinations for the formation of enzyme–MOF composites to diverse ends, such as catalysis, medicine, and in biosensor manufacturing, among others.…”

Section: Introductionmentioning

confidence: 99%

The Chemistry and Applications of Metal–Organic Frameworks (MOFs) as Industrial Enzyme Immobilization Systems

et al. 2022

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Enzymatic biocatalysis is a sustainable technology. Enzymes are versatile and highly efficient biocatalysts, and have been widely employed due to their biodegradable nature. However, because the three-dimensional structure of these enzymes is predominantly maintained by weaker non-covalent interactions, external conditions, such as temperature and pH variations, as well as the presence of chemical compounds, can modify or even neutralize their biological activity. The enablement of this category of processes is the result of the several advances in the areas of molecular biology and biotechnology achieved over the past two decades. In this scenario, metal–organic frameworks (MOFs) are highlighted as efficient supports for enzyme immobilization. They can be used to ‘house’ a specific enzyme, providing it with protection from environmental influences. This review discusses MOFs as structures; emphasizes their synthesis strategies, properties, and applications; explores the existing methods of using immobilization processes of various enzymes; and lists their possible chemical modifications and combinations with other compounds to formulate the ideal supports for a given application.

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Metal-organic frameworks with different dimensionalities: An ideal host platform for enzyme@MOF composites

Cited by 166 publications

References 101 publications

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Advances in 3D Gel Printing for Enzyme Immobilization

The Chemistry and Applications of Metal–Organic Frameworks (MOFs) as Industrial Enzyme Immobilization Systems

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Metal-organic frameworks with different dimensionalities: An ideal host platform for enzyme@MOF composites

Cited by 166 publications

References 101 publications

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO2 Capture

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO2 Capture

Advances in 3D Gel Printing for Enzyme Immobilization

The Chemistry and Applications of Metal–Organic Frameworks (MOFs) as Industrial Enzyme Immobilization Systems

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Product

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Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture

Tailoring the Properties of Self-Assembled Carbonic Anhydrase Supraparticles for CO₂ Capture