Metallo-β-Lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan

Nishio, Hisaaki; Komatsu, Masaru; Shibata, Naohiro; Shimakawa, Kouichi; Sueyoshi, Noriyuki; Ura, Toshiro; Satoh, Kaori; Toyokawa, Masahiro; Nakamura, Tatsuya; Wada, Yasunao; Orita, Tamaki; Kofuku, Tomomi; Yamasaki, Katsutoshi; Sakamoto, Masako; Kinoshita, Shohiro; Aihara, Masanori; Arakawa, Yoshichika

doi:10.1128/jcm.42.11.5256-5263.2004

Cited by 92 publications

(65 citation statements)

References 43 publications

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“…In contrast to the results of this study, MBL producers and MDRP strains have been found at considerably higher frequencies in previous studies of human isolates in Japan (3)(4)(5)9). Furthermore, IMP-45-producing P. aeruginosa of canine origin was isolated in China, suggestive of a human-to-dog transfer (15).…”

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confidence: 55%

“…The MIC criteria for the first screening of MBL producers (8 μg mL IPM or 16 μg mL CAZ) were used for all isolates as reported previously (3). Simultaneously, we performed a sodium mercaptoacetic acid (SMA) inhibition test to detect MBL producers from all isolates using 2 commercially prepared Kirby-Bauer disks: 1 containing 30 mg CAZ and 1 containing 3 mg SMA (Eiken Chemical, Tokyo, Japan), as described by the manufacturer.…”

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confidence: 99%

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Antimicrobial Resistance of <i>Pseudomonas aeruginosa</i> Isolated from Dogs and Cats in Primary Veterinary Hospitals in Japan

Yukawa

Tsuyuki²,

Satō

et al. 2017

Jpn J Infect Dis

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confidence: 55%

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confidence: 99%

Antimicrobial Resistance of <i>Pseudomonas aeruginosa</i> Isolated from Dogs and Cats in Primary Veterinary Hospitals in Japan

Yukawa

Tsuyuki²,

Satō

et al. 2017

Jpn J Infect Dis

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“…Among the clinically isolated P. aeruginosa, 1.7% in 1,058 isolates had metallo-β-lactamase genes with integron1, which could frequently form cassettes consisting of the genes of aminoglycoside modifying enzymes, or other β-lactamases resulting in several drug resistances (24). In a laboratory-based surveillance in Japan conducted during 1992-2002, metallo-β-lactamase-producing P. aeruginosa were identified at a rate of 0.4-1.9% (3,4,12,26). These results together with our observation suggest that the incidence of metallo-β-lactamase-positive P. aeruginosa has not increased in Japan.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Imipenem‐Resistant Pseudomonas aeruginosa in Hiroshima, Japan

Ohara

Kouda

Onodera

et al. 2007

Microbiology and Immunology

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Nosocomial infections by Pseudomonas aeruginosa continue to be a widespread problem in Asia, North and South America, and Europe. Carbapenems are the most potent β-lactams against P. aeruginosa because its strong affinity to penicillin binding proteins, stability against most serine β-lactamases and high permeability across the outer membrane (7,8). However, intensive use of the carbapenems facilitated the emergence of carbapenem-resistant P. aeruginosa. Low level resistance to imipenem (MIC, 8 to 32 µg/ml) in P. aeruginosa is mostly due to reduced uptake of the drug as a result of loss of the porin OprD (8). This mechanism correlates with continued expression of AmpC β-lactamase. Resistance to meropenem but not imipenem may also arise via overexpression of the MexA-MexBOprM efflux pump (14). High level resistance to carbapenems (MICϾ32 µg/ml) can be caused by the presence of metallo-β-lactamase, which exhibits a broadspectrum resistance to β-lactams including carbapenems. Metallo-β-lactamase-producing P. aeruginosa Abstract: Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla IMP-1 or blaVIM-2 allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-␤-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the SpeI-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-␤-lactamase producing P. aeruginosa strains in Japanese hospitals.

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“…Our previously reported survey data from the Kinki region of Japan revealed the prevalence of ESBLs and plasmid-mediated MBLs (21,30); however, the epidemiology of PABLs remains unknown. For this reason, a laboratory-based surveillance study was conducted to determine the presence and prevalence of PABLs among members of the family Enterobacteriaceae.…”

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confidence: 99%

Laboratory Surveillance for Prospective Plasmid-Mediated AmpC β-Lactamases in the Kinki Region of Japan

Yamasaki

Komatsu²,

Abe

et al. 2010

J Clin Microbiol

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Extended-spectrum ␤-lactamases, plasmid-mediated AmpC ␤-lactamases (PABLs), and plasmid-mediated metallo-␤-lactamases confer resistance to many ␤-lactams. In Japan, although several reports exist on the prevalence of extended-spectrum ␤-lactamases and metallo-␤-lactamases, the prevalence and characteristics of PABLs remain unknown. To investigate the production of PABLs, a total of 22,869 strains of 4 enterobacterial species, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis, were collected during six 6-month periods from 17 clinical laboratories in the Kinki region of Japan. PABLs were detected in 29 (0.13%) of 22,869 isolates by the 3-dimensional test, PCR analysis, and DNA sequencing analysis. PABL-positive isolates were detected among isolates from 13 laboratories. Seventeen of 13,995 (0.12%) E. coli isolates, 8 of 5,970 (0.13%) K. pneumoniae isolates, 3 of 1,722 (0.17%) K. oxytoca isolates, and 1 of 1,182 (0.08%) P. mirabilis isolates were positive for PABLs. Of these 29 PABL-positive strains, 20 (69.0%), 6 (20.7%), 2 (6.9%), and 1 (3.4%) carried the genes for CMY-2, DHA-1, CMY-8, and MOX-1 PABLs, respectively. Pattern analysis of randomly amplified polymorphic DNA and pulsed-field gel electrophoretic analysis revealed that the prevalence of CMY-2-producing E. coli strains was not due to epidemic strains and that 3 DHA-1-producing K. pneumoniae strains were identical, suggesting their clonal relatedness. In conclusion, the DHA-1 PABLs were predominantly present in K. pneumoniae strains, but CMY-2 PABLs were predominantly present in E. coli strains. The present findings will provide significant information to assist in preventing the emergence and further spread of PABL-producing bacteria.␤-Lactamase production is the most important factor for ␤-lactam resistance in Gram-negative rods (16). Plasmid-mediated ␤-lactamases, such as extended-spectrum ␤-lactamases (ESBLs), plasmid-mediated AmpC ␤-lactamases (PABLs), and plasmid-mediated metallo-␤-lactamases (MBLs), hydrolyze broad-spectrum ␤-lactams. Detection of these plasmidmediated ␤-lactamase-producing isolates is important for epidemiological studies and hospital infection control, because plasmid-mediated genes can spread to other organisms.The Study of Bacterial Resistance in the Kinki Region of Japan (SBRK) Antimicrobial Surveillance Program was established in 1997 to monitor the predominant pathogens and antimicrobial resistance patterns of nosocomial and communityacquired infections via a broad network of clinical laboratories differing in geographic location and size. Our previously reported survey data from the Kinki region of Japan revealed the prevalence of ESBLs and plasmid-mediated MBLs (21, 30); however, the epidemiology of PABLs remains unknown. For this reason, a laboratory-based surveillance study was conducted to determine the presence and prevalence of PABLs among members of the family Enterobacteriaceae.

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Metallo-β-Lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan

Cited by 92 publications

References 43 publications

Antimicrobial Resistance of <i>Pseudomonas aeruginosa</i> Isolated from Dogs and Cats in Primary Veterinary Hospitals in Japan

Antimicrobial Resistance of <i>Pseudomonas aeruginosa</i> Isolated from Dogs and Cats in Primary Veterinary Hospitals in Japan

Molecular Characterization of Imipenem‐Resistant Pseudomonas aeruginosa in Hiroshima, Japan

Laboratory Surveillance for Prospective Plasmid-Mediated AmpC β-Lactamases in the Kinki Region of Japan

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